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Application of p300 activator CTPB and derivative in improving collagen Col17A1 expression

A technology of collagen and activator, applied in the direction of drug combination, skin diseases, etc., can solve problems such as hair loss and decline in regeneration function of hair follicle stem cells

Inactive Publication Date: 2018-12-21
潘治忠
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Digestion of Col17A1 leads to decreased regenerative function of hair follicle stem cells and leads to hair loss

Method used

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  • Application of p300 activator CTPB and derivative in improving collagen Col17A1 expression
  • Application of p300 activator CTPB and derivative in improving collagen Col17A1 expression
  • Application of p300 activator CTPB and derivative in improving collagen Col17A1 expression

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Study of the effect of addition of CTPB (Santa Cruz, USA) on Col17A gene expression in fibroblast 10T 1 / 2.

[0028] The Bioinformatics Group of Tsinghua University released a super enhancer database (http: / / asntech.org / dbsuper / index.php), using the Detailed Search function, the parameters are as follows: mouse (mm9), select allcell / tissue type, The gene name Col17A1. We found that the super-enhancer that regulates the Col17A1 gene is specific to hair follicle stem cells. This super-enhancer is inactive in the other 22 types of cells. The results are shown in figure 1 . Since hair follicle cells can also be differentiated from fibroblasts, we used fibroblasts to study the effect of CTPB on Col17A1 gene expression. In addition, the expression level of Col17A1 gene will also decrease in the aging process of mouse skin, the results are shown in figure 2 .

[0029] In addition, using the C57BL / 6J mouse experimental model, 1 cm 2Then use Trizol (Life Technolo...

Embodiment 2

[0031] Example 2: To study the effect of exogenously added CTPB on the treatment of hair loss or promotion of hair growth.

[0032] 1. Establishment of mouse hair loss model and the effect of CTPB on hair regeneration.

[0033] Using 6 aging (20 months) C57BL / 6 mice, the mice were randomly divided into control group and experimental group, and 100 μl of CTPB (concentration 200nM, dissolved in PEG-400 / ethanol / water ratio of 40% was applied on the skin every day) / 20% / 40%), the mouse body weight is 35g / only) as the treatment group (PEG-400 / ethanol / water, the ratio is 40% / 20% / 40% is the control group), after treatment, take pictures to observe the treatment alopecia see results image 3 ,like image 3 Compared with the 20-month-old and 6-month-old mice, the 20-month-old mice had obvious hair loss, and the hair loss in the CTPB-applied 14-day treatment group was significantly improved compared with the control group.

[0034] 2. The effect of CTPB on the expression of Col17A1 i...

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Abstract

The invention relates to the fields of pharmacy and wash supplies, and particularly discloses application of a CTPB activator. The CTPB activator can be used for preparing a hair restorer and / or a hair loss curing agent and for preparing a medicine for curing hair loss / promoting hair increase.

Description

technical field [0001] The invention relates to the fields of pharmacy and toiletries, and more specifically, the invention discloses the use of a CTPB activator. Background technique [0002] Hair follicle stem cells promote hair follicle regeneration and hair growth, and also repair damaged cuticles. The differentiation of hair follicle stem cells is regulated by various pseudoinheritance, including enhancer (enhancer) and marker histone H3 (H3K27Ac). Enhancers are short DNA sequences that regulate the expression of a target gene. When the function of enhancers is affected, it leads to various diseases, such as autoimmune diseases and cancer. There are currently different methods to identify enhancers, such as ATAC-seq and immunoprecipitation combined with high-throughput sequencing chromatin immunoprecipitation with high-throughput sequencing (ChIP-seq), which can find out how a fixed target is connected in the genome Such as histone marks, transcription factors or co-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/165A61P17/14
CPCA61K31/165A61P17/14
Inventor 苏锦熙
Owner 潘治忠