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Time resolution fluorescent immunochromatographic test strip for detecting butralin, and preparation method and application thereof

A time-resolved fluorescence and immunochromatography test paper technology, which is applied in the fields of analytical materials, biological testing, fluorescence/phosphorescence, etc., can solve the problems of high professional requirements for operators, expensive detection costs, cumbersome steps, etc., and achieve a wide range of applications , short detection time and simple operation

Active Publication Date: 2018-12-21
ZHENGZHOU TOBACCO RES INST OF CNTC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the above methods require advanced detection instruments, the detection costs are expensive, the steps are cumbersome, time-consuming, and require high professionalism of operators, they are not suitable for high-throughput rapid screening detection in grassroots enterprises and institutions.

Method used

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  • Time resolution fluorescent immunochromatographic test strip for detecting butralin, and preparation method and application thereof
  • Time resolution fluorescent immunochromatographic test strip for detecting butralin, and preparation method and application thereof
  • Time resolution fluorescent immunochromatographic test strip for detecting butralin, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 The composition of the time-resolved fluorescent immunochromatographic test strip for detecting Zhongbuling

[0039] 1. Test strips

[0040] see figure 1 : The test strip is composed of a bottom plate, a sample absorbent pad, a conjugate release pad, a nitrocellulose membrane and an absorbent pad;

[0041] The sample absorbent pad 1, the conjugate release pad 2, the nitrocellulose membrane 3 and the water absorbent pad 4 are overlapped and pasted on the bottom plate 7 in sequence, and 1 / 3 of the conjugate release pad is covered by the sample absorbent pad from the starting end , the end of the conjugate release pad is connected to the beginning of the nitrocellulose membrane, the end of the nitrocellulose membrane is connected to the beginning of the absorbent pad, the beginning of the sample absorbent pad is aligned with the beginning of the PVC bottom plate, and the end of the absorbent pad is connected to the end of the PVC bottom plate alignment;

[004...

Embodiment 2

[0044] Example 2 Preparation of Time-Resolved Fluorescence Immunochromatographic Test Strips for Detecting Zhongbutaline

[0045] The preparation method of the time-resolved fluorescent immunochromatographic test strip for detecting Zhongbuling mainly comprises the following steps:

[0046] 1) Preparation of the conjugate release pad: use commercially available fluorescent microspheres to label secbutaline monoclonal antibody, and dilute it with a specific buffer system, soak the conjugate release pad in the dilution buffer, and vacuum freeze-dry Post-preparation;

[0047] 2) Preparation of nitrocellulose membrane: Spray the secbutyrine hapten-carrier protein conjugate onto the detection area on the nitrocellulose membrane to make the detection area; spray goat anti-mouse anti-antibody onto the nitrocellulose membrane The scope of the quality control area above is made into the quality control area;

[0048] 3) Assembling and cutting: Paste the sample absorption pad, the con...

Embodiment 3

[0102] Example 3 Application of Time-Resolved Fluorescence Immunochromatographic Test Strips for Detecting Zhongbuling

[0103] 1. Tobacco sample pretreatment

[0104] Weigh 2.0±0.05 g of pulverized tobacco samples into a polystyrene centrifuge tube; add 10 mL of 50% methanol aqueous solution, and use a homogenizer to fully break it up to obtain a sample liquid; pipette 75 μL of sample liquid and 425 μL of The ionized water was mixed and waited for inspection.

[0105] 2. Test with test strips

[0106] Draw 100 μL of the sample solution to be tested and drop it vertically into the sample hole of the test paper card. When the liquid flows, start timing and react for 10 minutes; insert the test paper card into the carrier of the KFT-100A fluorescence detector, and select the sample to be tested through the touch screen. item, press the "Start Test" button, the fluorescence detector will automatically scan the test paper card, and read or print the test results on the display s...

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Abstract

The invention discloses a time resolution fluorescent immunochromatographic test strip for detecting butralin, and a preparation method and application thereof. The time resolution fluorescent immunochromatographic test strip comprises a bottom plate and further comprises a sample absorbent pad, a combination releasing pad, a nitrocellulose membrane and a water absorbent pad which are lapped and pasted on the bottom plate in sequence. A butralin monoclonal antibody labelled by fluorescent microspheres is embedded into the combination releasing pad, and a detecting area and a quality control area are fixed to the nitrocellulose membrane. A butralin antigen-carrier protein conjugate is sprayed in the detecting area, and an anti-sheep and anti-mouse antibody is sprayed in the quality controlarea. A butralin antigen is obtained in the mode of reacting of 4-chlorine-3,5-binitro phenylacetic acid with butyl amine. The invention further provides the preparation method of the test strip and amethod for detecting the butralin in samples through the test strip. The time resolution fluorescent immunochromatographic test strip and the detecting method provided by the invention have the advantages that operation is easy, sensitivity is high, the detecting speed is high, and the cost is low, and the butralin in the large-batch samples can be detected quaickly and monitored on site.

Description

technical field [0001] The invention belongs to the field of pesticide residue detection, and in particular relates to a time-resolved fluorescent immunochromatographic test strip for detecting Zhongbuling in tobacco and tobacco products, a preparation method and application thereof. Background technique [0002] Butralin (Butralin) is also known as dimethamine, bibutralin, nifediamine, bidarin, and budling. The chemical name is N-sec-butyl-4-tert-butyl-2,6-dinitro Aniline, the molecular formula is C 14 h 21 N 3 o 4 . Zhongbuling is a dinitroaniline herbicide. The pure product is an orange-yellow crystal. It is easily soluble in organic solvents but hardly soluble in water. It is volatile and easy to decompose when exposed to light. It has low toxicity to humans and animals. The drug is a selective preemergent herbicide, and its effect is similar to that of trifluralin. After the medicament enters the plant, it mainly inhibits the cell division of the meristem, thereby...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/58G01N33/533G01N21/64
CPCG01N21/6408G01N33/533G01N33/558G01N33/582G01N33/585
Inventor 陈黎范子彦刘惠民唐纲岭崔华鹏樊美娟聂聪赵乐郭军伟王洪波
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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