Phyllostachys heterocycle PeVQ28 protein and coding gene and application thereof

A coding gene, technology of moso bamboo, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problem that the function of VQ gene has not been reported, and achieve the effects of enhanced salt resistance, improved salt resistance, and good application prospects

Active Publication Date: 2018-12-25
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the published VQ genes and their homologous genes are derived from model plants or herbaceous plants. These plants are mostly annual plants, and the function of VQ genes in moso bamboo has not yet been reported.

Method used

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  • Phyllostachys heterocycle PeVQ28 protein and coding gene and application thereof
  • Phyllostachys heterocycle PeVQ28 protein and coding gene and application thereof
  • Phyllostachys heterocycle PeVQ28 protein and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A Protein Sequence Encoded by PeVQ28 Gene of Phyllostachys pubescens

[0026] Use primers software, carry out protein sequence translation (SEQID No.1) ( figure 1 ).

Embodiment 2

[0028] Analysis of the induced expression pattern of PeVQ28, a salt-tolerance-related gene in Phyllostachys pubescens

[0029] (1) Stress treatment and total RNA extraction

[0030] Use 100mM sodium chloride solution, 150mM sodium chloride solution and 200mM sodium chloride solution to irrigate annual moso bamboo seedlings respectively, keeping other nutritional conditions constant. Use scissors (cleaned and disinfected with 75% alcohol in advance) to take leaves of Phyllostachys pubescens 0, 1, 3, 6, 12 and 24 hours after various treatments, put them into 2mL dof tubes containing RNA protection solution, and store them for later use. The time period and sample plants were repeated 3 times. Total RNA was extracted by Trizol method, the detailed steps are as follows:

[0031] (1) Take out the spare leaves of Phyllostachys pubescens from the RNA protection solution, put them in the filter paper to absorb the protection solution as much as possible to avoid affecting it, transf...

Embodiment 3

[0074] Cloning of Salt Tolerance Related Gene PeVQ28 in Phyllostachys pubescens

[0075] According to the CDS sequence of the PeVQ28 gene published on the Moso bamboo genome website, the primers for PCR amplification of this fragment were designed, the upstream primer was added with a Sma I restriction site, and the downstream primer was added with a Sal I restriction site.

[0076] The primer sequences are as follows:

[0077] PeVQ28-F5′-ATA CCCGGG ATGGGGGAGTACCACAG-3′

[0078] PeVQ28-R5-GCC GTC GAC TTAAGATGCGTACATTTCAC-3′

[0079] The first-strand cDNA was reverse-transcribed from total RNA of leaves of Phyllostachys pubescens as a template, and PCR amplification was performed with upstream and downstream primers. The PCR reaction system is shown in Table 1:

[0080] Table 1

[0081]

[0082] The PCR reaction conditions were: pre-denaturation: 98°C 10min; denaturation: 98°C 10s; annealing: 58°C 30s; extension: 72°C 1min, 39 cycles; total extension: 72°C 10min.

[...

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Abstract

The invention discloses Phyllostachys heterocycle PeVQ28 protein and a coding gene and application thereof. The amino acid sequence of the protein is as shown in SEQ ID No. 1, coding DNA sequence (CDS) is as shown in SEQ ID No. 2, and the cDNA sequence overall length of the Phyllostachys heterocycle PeVQ28 gene is as shown in SEQ ID No. 3. The PeVQ28 gene has high expression under different-degreesalt stress induction. The fact that the salt resistance of transgenic wild arabidopsis obtained by using a agrobacterium tumefaciens mediated inflorescence impregnating method to transfer a PeVQ28 gene expression vector into wild Arabidopsis is evidently increased shows that the PeVQ28 gene can increase the salt resistance of the transgenic Arabidopsis. A plant material obtained by using the Phyllostachys heterocycle PeVQ28 gene expression vector has certain adaptability to an external salt stress environment, and promising application prospect is achieved.

Description

technical field [0001] The invention relates to the field of plant molecular biology, in particular to a PeVQ28 protein of moso bamboo and its coding gene and application. Background technique [0002] China is very rich in bamboo species. According to statistics, there are about 39 genera and more than 500 species. Whether it is the source of bamboo, the planting area, or the stock volume and output of bamboo forests, it ranks first in the world. It can be said that China is the most important in the world. Countries that produce bamboo. Moso bamboo is also a kind of forest resource, which occupies a very important position in southern my country. Due to its fast growth rate, short period of reaching usable standards, and considerable output, it can replace some types of slow-growing forest resources. With the development of science and technology The development of Moso bamboo is more widely used. Due to the characteristics of moso bamboo, its use in buildings has gradual...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/11C12N15/70C12N1/21A01H5/00A01H6/20
CPCC07K14/415C12N15/8273
Inventor 项艳程欣然王玉娇熊瑞吴敏严涵薇
Owner ANHUI AGRICULTURAL UNIVERSITY
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