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In-situ coating-modified bacterial cellulose hydrogel and method for preparing same

A bacterial cellulose and hydrogel technology, applied in biochemical equipment and methods, coatings, microorganism-based methods, etc., can solve problems such as difficult control, time-consuming and energy-consuming, and cumbersome processes, and achieve the effect of simple process.

Active Publication Date: 2018-12-25
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a method for in-situ coating modification of bacterial cellulose hydrogel, aiming at the problems of cumbersome process, time-consuming and energy-consuming, difficult to control and other problems of the existing bacterial cellulose hydrogel functional modification method

Method used

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  • In-situ coating-modified bacterial cellulose hydrogel and method for preparing same
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  • In-situ coating-modified bacterial cellulose hydrogel and method for preparing same

Examples

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Embodiment 1

[0031] Example 1: Preparation of Chitosan In-Situ Coating Modified Bacterial Cellulose Hydrogel

[0032] The preparation process is as image 3 shown, with the following steps:

[0033] (1) Preparation of standard HS culture solution: Weigh 4 g of glucose, 1 g of yeast powder, 0.54 g of disodium hydrogen phosphate, and 0.23 g of citric acid in ultrapure water, and prepare 200 mL of standard HS culture solution;

[0034] (2) Add 2 g of chitosan (molecular weight is about 100,000) to the standard HS culture solution, stir well, and after being dispersed and mixed, sterilize by autoclaving, and the chitosan turns brown.

[0035] (3) Inoculate 5 mL of Acetobacter xylinum (American Standard Bacteria Bank ATCC 53582) bacterium solution into the standard HS culture solution containing chitosan described in step (1), and cultivate it for 10 days in a constant temperature incubator at 30°C to obtain chitosan Sugar-coated modified bacterial cellulose hydrogel, shown as a two-layer str...

Embodiment 2

[0040] Example 2: Preparation of Chitosan In-Situ Coating Modified Bacterial Cellulose Hydrogel

[0041] Based on Example 1, the difference is: change the amount of chitosan into 4g in step (2), change the cultivation time in step (3) into 15 days, and all the other are the same as in Example 1.

Embodiment 3

[0042] Example 3: Preparation of chitosan in-situ coating modified bacterial cellulose hydrogel

[0043] Based on Example 1, the difference is: change the amount of chitosan into 6g in step (2), change the cultivation time in step (3) into 5 days, and all the other are the same as in Example 1.

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Abstract

The invention relates to in-situ coating-modified bacterial cellulose hydrogel and a method for preparing the same, and belongs to the technical field of biological materials. The in-situ coating-modified bacterial cellulose hydrogel comprises bacterial cellulose layers and modified layers. The corresponding modified layers are modified on at least one surface of each bacterial cellulose layer andare water-insoluble biological macromolecule layers; the water-insoluble biological macromolecule layers are connected with the bacterial cellulose layers by physical adsorption and interaction forceof hydrogen bonds. Water-insoluble biological macromolecules are added into culture solution, and bacterial cellulose hydrogel with a single modified surface can be obtained by means of bacterial fermentation; the bacterial cellulose hydrogel with the single modified surface can be taken out and can be placed on gas-solution interfaces of the culture solution upside down to continue being subjected to culture, so that bacterial cellulose hydrogel with double modified surfaces can be obtained. The in-situ coating-modified bacterial cellulose hydrogel and the method have the advantages that thesurfaces of the bacterial cellulose hydrogel can be modified while the bacterial cellulose hydrogel is synthesized by the aid of in-situ coating modification processes, complicated chemical procedures can be omitted, organic solvents or professional preparation equipment are not required, accordingly, energy can be saved, environments can be protected, and the in-situ coating-modified bacterial cellulose hydrogel and the method are simple and convenient and are high in efficiency.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a method for in-situ coating modification of bacterial cellulose hydrogel. Background technique [0002] Bacterial cellulose (BC) refers to cellulose synthesized by microorganisms such as Acetobacter xylinum, and its hydrogel has fine network structure, high mechanical strength, high water content, good biocompatibility and biodegradability, etc. features. Therefore, as an excellent matrix material, it is widely used in the construction of functional materials and devices such as biomedicine and optoelectronics. Among them, how to functionalize bacterial cellulose hydrogel is the key. [0003] At present, the surface functional modification of bacterial cellulose hydrogel mainly includes physical doping and chemical modification. For example, Shi Zhijun et al. made polyaniline permeate into bacterial cellulose hydrogel by physical doping method to obtain conducti...

Claims

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Application Information

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IPC IPC(8): C08J7/04C08J3/075C08L1/02C12P19/04C12R1/01C12R1/41
CPCC08J3/075C08J7/0427C08J2301/02C08J2405/00C08J2489/00C12P19/04
Inventor 杨光肖林黄丽霞付丽娜刘莉
Owner HUAZHONG UNIV OF SCI & TECH
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