Peripheral blood detection method of hepatitis B virus integration in liver

Abstract

The invention provides a method for detecting the hepatitis B virus integration condition, which is used for detecting by taking free DNA in peripheral blood as a sample. According to the method, therisk of complications caused by traditional methods, such as surgical biopsy, aspiration biopsy and the like can be avoided, the safety can be improved, periodical monitoring with high frequency can be realized, and heterogeneity bias of tissue sampling can be avoided. The method has good specificity and high sensitivity, can detect a fragment in a peripheral blood sample of a patient with liver cancer after 100 percent of HBV infection, and minimally detect a fragment in all cfDNAs extracted from the plasma of 5ml of peripheral blood.

Description

technical field

[0001] The invention relates to the detection of the integration status of the hepatitis B virus, in particular to the application of free DNA in peripheral blood in the detection of the integration status of the hepatitis B virus. Background technique

[0002] Hepatitis B Virus (HBV), as a retrovirus, has the ability to integrate into the host genome. It was first reported in the early 1980s, and there are many studies on liver cancer genomes. 1-13 . The integration ability of HBV is not necessary for its life cycle (Life Cycle) and infecting the host. Currently, there is no gene encoding a viral protein with integrase activity in the hepatovirus genome 14 , earlier studies had considered HBV integration events to be rare events distributed randomly across the genome 15 . Studies on the integration mechanism of HBV suggest that the main form of virus integration into host genes is linear double-stranded DNA (double strand linear DNA, dsL DNA) 16 , is an ...

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