Preparation method and method of hog cholera-ring-mixed antigen, hog cholera-ring subunit vaccines and preparation method thereof

A technology of mixing antigens and doublets, which is used in biochemical equipment and methods, medical preparations containing active ingredients, vaccines, etc., can solve the problems of insufficient antigen concentration, cumbersome preparation process, affecting immune effect, etc., and saves money. The effect of human and material resources, high antibody titer and simple process

Pending Publication Date: 2018-12-28
TECON BIOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the prevention and control of the above two diseases on the market use a single vaccine that only targets one of the diseases, and the immunization process wastes manpower and material resources; , most of which are whole-virus vaccines, the two viruses are cultured separately, and then the venom is collected and mixed, which wastes a high cost in the antigen preparation process, and the mixing of the two virus solutions often leads to insufficient antigen concentration or the need to increase the virus concentration process , the preparation process is relatively cumbersome. After mixing the two virus culture solutions, relatively more miscellaneous proteins are released inside the cells, and the miscellaneous proteins are often immunogenic. Antigen competes with immunoglobulin, which affects the immune effect, and its preparation process also causes a waste of manpower, material resources and time

Method used

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  • Preparation method and method of hog cholera-ring-mixed antigen, hog cholera-ring subunit vaccines and preparation method thereof
  • Preparation method and method of hog cholera-ring-mixed antigen, hog cholera-ring subunit vaccines and preparation method thereof
  • Preparation method and method of hog cholera-ring-mixed antigen, hog cholera-ring subunit vaccines and preparation method thereof

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preparation example Construction

[0048] According to a first aspect of the present invention, a method for preparing a hog fever-circle mixed antigen is provided, comprising the steps of:

[0049] Inoculate the virus mixture of the classical swine fever E2 protein recombinant virus and the porcine circovirus type 2 Cap protein recombinant virus in the cell culture to obtain the mixed antigen.

[0050] Classical swine fever is an acute, febrile, contagious infectious disease caused by the swine fever virus of the family Flaviviridae, which is highly contagious and lethal, and can occur all year round. Swine fever is currently one of the key diseases in livestock diseases, and the outbreak of the disease is likely to cause heavy losses in the breeding industry.

[0051] Porcine circovirus disease, caused by porcine circovirus, has a variety of clinical symptoms, such as anorexia, dyspnea or sluggishness, and has a high mortality rate. It is also one of the key diseases in livestock diseases. one.

[0052] The...

Embodiment 1

[0121] Co-cultivation of recombinant baculovirus with classical swine fever E2 protein and recombinant baculovirus with porcine circovirus type 2 Cap protein: Add shake flasks to 14L tanks to culture 2.5L with a density of about 1 million / ml and cell viability greater than 95 % more than Sf-9 cells, adjust the cell density in the reaction tank to about 1 million / ml by adding fluid. The dissolved oxygen (Do) in the bioreactor was set at 40%, the temperature was 27° C., the pH was 6, the volume of the cell culture in the reactor was controlled to be 10 L, and the rotation speed was 100 rpm. When the cell density of the 14L tank is about 1 million / ml, insert classical swine fever E2 baculovirus and PCV2 recombinant baculovirus according to the amount of MOI=0.1. The operating parameters of the reactor were recorded every day, and samples were taken and counted on the 3rd to 4th day. Take a sample every day afterwards, calculate the cell viability rate with a cell counter, collec...

Embodiment 2

[0126] The only difference from Example 1 is that the virus mixture of the recombinant baculovirus of classical swine fever E2 protein and the recombinant baculovirus of porcine circovirus type 2 Cap protein is not obtained by joint culture, but after being cultured separately, the virus is harvested, and then All were inoculated in High5 cell culture at MOI=0.1.

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Abstract

The invention provides a preparation method and method of hog cholera-ring-mixed antigen, hog cholera-ring subunit vaccines and a preparation method thereof, and relates to the technical field of biological products. The method comprises the following step of inoculating virus mixing liquid of hog cholera E2 protein recombinant viruses and porcine circovirus II-type Cap protein recombinant virusesinto a cell culture for culturing to obtain the mixed antigen. The preparation method of the hog cholera-ring-mixed antigen, hog cholera-ring subunit vaccines relieves the complicated working procedures that in a conventional technology, two kinds of viruses are cultured in batches and compounded after being gained. The valence of antibody after the vaccines prepared from the mixed antigen are used for vaccine immunity is high.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a method for preparing a hog fever-circle mixed antigen and its product, a hog fever-circle dual subunit vaccine and a preparation method thereof. Background technique [0002] Classical swine fever is an acute, febrile, contagious infectious disease caused by swine fever virus belonging to the family Flaviviridae. It is highly contagious and lethal and can occur all year round; porcine circovirus disease, Caused by porcine circovirus, it is a disease with multiple clinical symptoms, such as anorexia, dyspnea, or slow movement, and has a high mortality rate; the above two diseases are key diseases for the prevention and control of my country's animal husbandry industry. [0003] Most of the prevention and control of the above two diseases on the market use a single vaccine that only targets one of the diseases, and the immunization process wastes manpower and material ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61K39/295A61P31/20A61P31/14
CPCA61K39/12A61P31/14A61P31/20A61K2039/70A61K2039/5252A61K2039/552C12N2750/10034C12N2770/24334
Inventor 贺笋徐龙飞李俊辉王遵宝李延涛
Owner TECON BIOLOGY CO LTD
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