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Method for preparing saxitoxin by utilizing fermentation of toxin-producing marine sulfitobacter sp.

A technology of sulfite bacillus and saxitoxin, which is applied in the field of preparation of saxitoxin, can solve the problems of long cultivation period, short period and high cost, and achieve the effect of low cost, short period and small workload

Inactive Publication Date: 2018-12-28
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to overcome the problems of long cultivation period, high cost and special algae cultivation equipment in the method for obtaining saxitoxin in the prior art, and provides a utilization method with high yield, short period and low cost. Method for preparing saxitoxin by fermentation of toxin-producing marine sulfite bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing saxitoxin by fermentation of toxin-producing marine sulfite bacteria, wherein the sulfite bacteria are sulfite bacteria ( Sulfitobacter sp.) Z1-D strain, the preservation number is CCTCC AB 2016296, purchased from China Center for Type Culture Collection, the method described includes the following steps:

[0028] (1) Strain activation: Bacillus sulfite ( Sulfitobacter sp.) Z1-D was inoculated in the slant medium, and the activated strain was cultured at 27°C for 2 days. The formula of the plate medium in step (1) was as follows: ammonium sulfate 0.8g, yeast extract 0.2g, sodium alginate 0.6g, 0.1g iron phosphate, 2g agar, add natural seawater to 100ml, pH6.9.

[0029] (2) Preparation of seed solution: Prepare 1L of seed medium, add it to a 5L seed tank, heat it with steam, sterilize at 121°C for 30 minutes, cool to 28°C, pick the activated single colony, and inoculate it in the seed tank by flame method In the liquid seed medium in the seed t...

Embodiment 2

[0034] A method for preparing saxitoxin by fermentation of toxin-producing marine sulfite bacteria, wherein the sulfite bacteria are sulfite bacteria ( Sulfitobacter sp.) Z1-D strain, the preservation number is CCTCC AB 2016296, purchased from China Center for Type Culture Collection, the method described includes the following steps:

[0035] (1) Strain activation: Bacillus sulfite ( Sulfitobacter sp.) Z1-D was inoculated in the slant medium, and the activated strain was cultured at 31°C for 4 days. The formula of the plate medium in step (1) was as follows: ammonium sulfate 1.2g, yeast extract 0.5g, sodium alginate 1.0g, 0.5g iron phosphate, 2g agar, add natural seawater to 100ml, pH7.3.

[0036] (2) Preparation of seed solution: Prepare 1L of seed medium, add it to a 5L seed tank, heat it with steam, sterilize at 121°C for 30 minutes, cool to 28°C, pick the activated single colony, and inoculate it in the seed tank by flame method In the liquid seed medium in the seed t...

Embodiment 3

[0041] A method for preparing saxitoxin by fermentation of toxin-producing marine sulfite bacteria, wherein the sulfite bacteria are sulfite bacteria ( Sulfitobacter sp.) Z1-D strain, the preservation number is CCTCC AB 2016296, purchased from China Center for Type Culture Collection, the method described includes the following steps:

[0042] (1) Strain activation: Bacillus sulfite ( Sulfitobacter sp.) Z1-D was inoculated in the slant medium, and the activated strain was cultured at 28°C for 3 days. The formula of the plate medium in step (1) was as follows: ammonium sulfate 1.0g, yeast extract 0.4g, sodium alginate 0.8g, 0.3g iron phosphate, 2g agar, add natural seawater to 100ml, pH7.0.

[0043] (2) Preparation of seed solution: Prepare 1L of seed medium, add it to a 5L seed tank, heat it with steam, sterilize at 121°C for 30 minutes, cool to 28°C, pick the activated single colony, and inoculate it in the seed tank by flame method In the liquid seed medium in the seed t...

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PUM

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Abstract

The invention belongs to the field of marine microbial fermentation and particularly relates to a method for preparing saxitoxin by utilizing fermentation of toxin-producing marine sulfitobacter sp..The method comprises the following steps of bacterial strain activation, seed solution preparation, culture fermentation through fermentation tanks, extraction of fermentation liquor, purification ofa crude extract, namely the saxitoxin. Compared with the prior art, the method has the following advantages that the marine microbial strain sulfitobacter sp. Z1-D is used as a production strain, thesaxitoxin yield through strain fermentation is higher and can reach 61.5-72.8 micrograms / L, the fermentation time is 40-48 hours, and the energy consumption is saved; 2, the steps of the method are simple, the working amount is small, a period is shorter, the used agents and materials are agents for microbial fermentation tests and are harmless to human and friendly to the environment, the cost islow, and the method has better market prospect and economic value.

Description

technical field [0001] The invention belongs to the field of marine microbial fermentation, and in particular relates to a method for preparing saxitoxin by fermenting toxin-producing marine sulfite bacteria. Background technique [0002] Paralytic shellfish posoning (PSP) belongs to guanamine toxins, which is the most toxic, widely distributed, most threatening, and causes the most frequent poisoning events among known red tide toxins. It mainly includes Saxitoxin (STX) and its derivatives, which are typical sodium ion channel blockers. STX has important applications in harmful red tide detection, neurobiology, medical diagnosis, drug development, biochemical warfare agents and other research. In terms of drug development, STX's unique chemical structure and toxic mechanism of action have become an important tool drug for the study of cellular Na+ channels. It has anti-tumor, anti-virus, analgesic, anesthesia, antispasmodic, antihypertensive, antiasthmatic and other effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12R1/01
CPCC12P17/182
Inventor 张晓玲杨桥穆军蒋志伟张若男
Owner ZHEJIANG OCEAN UNIV