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Method for rapidly extracting potassium and sodium ions in plant

An extraction method and sodium ion technology, applied in the field of plant salt resistance and nutritional physiology research, can solve the problems of high cost and complicated operation, and achieve the effects of short time consumption, less reagent dosage and avoiding interference.

Inactive Publication Date: 2018-12-28
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The time used by these methods is basically more than several hours, and except for the boiling method, other methods use various acid solutions, the operation is complicated, and the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1 A method for rapid extraction of potassium and sodium ions in plants, the method refers to cutting the collected plant samples into small sections of ≤ 1 cm and then mixing; weighing 10 g of sample sections and putting them into a homogenizer, and then adding the sample 5 times the mass of ultrapure water, homogenized at a speed of 5000 r / min for 5 min, centrifuged and filtered to obtain plant sample extract; finally, draw 5 mL of plant sample extract, put it into a 25 mL volumetric flask, add 1 mL 0.1 mol / L aluminum sulfate solution, dilute to the mark with ultrapure water, and shake well.

[0012] Among them: the centrifugation condition refers to the rotational speed of 4000 r / min and the time of 12 min.

Embodiment 2

[0013] Example 2 A method for quickly extracting potassium and sodium ions in plants, the method refers to cutting the collected plant samples into small sections of ≤ 1 cm and then mixing; weighing 20 g of sample sections and putting them into a homogenizer, and then adding the sample Ultrapure water with 5 times the mass was homogenized at a speed of 20000 r / min for 2 min, centrifuged and filtered to obtain the plant sample extract; finally, draw 10 mL of plant sample extract, put it into a 25 mL volumetric flask, and add 1 mL 0.1 mol / L aluminum sulfate solution, dilute to the mark with ultrapure water, and shake well.

[0014] Among them: the centrifugation condition refers to the rotating speed of 6000 r / min and the time of 8 min.

Embodiment 3

[0015] Example 3 A method for quickly extracting potassium and sodium ions in plants, the method refers to cutting the collected plant samples into small sections of ≤ 1 cm and then mixing; weighing 15 g of sample sections and putting them into a homogenizer, and then adding the sample Ultrapure water with a mass of 5 times was homogenized at a speed of 10000 r / min for 3 min, centrifuged and filtered to obtain a plant sample extract; finally, draw 8 mL of plant sample extract, put it into a 25 mL volumetric flask, and add 1 mL 0.1 mol / L aluminum sulfate solution, dilute to the mark with ultrapure water, and shake well.

[0016] Among them: the centrifugation condition refers to the rotational speed of 5000 r / min and the time of 10 min.

[0017] The potassium and sodium ion content of the liquid obtained in Examples 1 to 3 was determined by using an inductively coupled plasma emission spectrometer.

[0018] Conditions of inductively coupled plasma optical emission spectrometer...

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Abstract

The invention relates to a method for rapidly extracting potassium and sodium ions in a plant. The method comprises the following steps: cutting a collected plant sample into small sections less thanor equal to 1cm for uniform mixing; weighing and placing 10 to 20 grams of the sample sections in a homogenizer, then adding ultrapure water in an amount which is 5 times the mass of the sample sections for homogenization, and performing centrifugation and filtering to obtain plant sample leachate; and sucking and containing 5 to 10 milliliters of the plant sample leachate in a 25mL volumetric flask, adding 1 milliliter of a 0.1mol / L aluminum sulfate solution, adding ultrapure water to a volume, and performing uniform shaking. According to the method, cell structures are rapidly damaged by useof high-speed homogenization to release the potassium and sodium ions in cells, the characteristics of low time consumption, convenience for operation and the like are achieved, and the potassium andsodium ions in the plant can be rapidly detected.

Description

technical field [0001] The invention relates to the technical field of plant salt resistance and nutritional physiology research, in particular to a method for quickly extracting potassium and sodium ions from plants. Background technique [0002] In the study of plant salt resistance and nutritional physiology, it is often necessary to determine inorganic ions such as sodium (Na) and potassium (K) in plant tissues. How to extract Na and K plasma from plant tissues, different researchers use different methods, totaling no less than a dozen. At present, the commonly used extraction methods include ashing method, boiling method, 0.5% nitric acid solution water bath extraction method, perchloric acid and concentrated nitric acid mixed solution water bath extraction method, 0.5 mol / L HCl solution water bath extraction method, etc. The time used by these methods is basically more than several hours, and except the boiling method, other methods all use various acid solutions, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 董琦赵静迟晓峰冀恬
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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