A method for detecting aluminum ions in cells by long-wave emission fluorescence imaging

Abstract

A method for detecting aluminum ions in cells by long-wave emission fluorescence imaging, using fluorescent probe L as fluorescent imaging to detect trace amounts of Al in cells 3+ Fluorescent imaging probes for the detection of trace amounts of Al in cells by long-wave emission fluorescence imaging 3+ , the chemical structural formula of the fluorescent probe L is: during detection, first use the fluorescent probe L solution and cell culture to make the fluorescent probe L enter the active cells, and then combine the active cells with the fluorescent probe L with Al 3+ Incubate to make the probe with Al 3+ React in active cells to generate compounds that can emit fluorescence at characteristic wavelengths, enabling probes to detect Al in active cells 3+ Ion staining imaging, using a laser confocal fluorescence microscope to observe the fluorescence images of active cells after culture. The advantages are: high selectivity and good sensitivity, fluorescence imaging detection of trace Al in cells at a longer emission wavelength 3+ , and has strong tissue penetration, avoiding photodamage and background fluorescence self-interference.

Description

technical field [0001] The invention relates to a method for detecting aluminum ions in cells by long-wave emission fluorescence imaging. Background technique [0002] It is well known that the development of chemical sensors for detecting metal ions has received much attention due to their important roles in medicine, living systems, and the environment. Aluminum (Al), the third most prevalent and abundant metallic element in the earth's crust, is widely exposed to people because it is dispersed in areas such as water treatment, food additives, storage tools or cookware, pharmaceuticals, and light alloys. At the same time, Al 3+ It is also an essential element in the life system, but excessive intake of Al 3+ It will affect the absorption of calcium in the intestine, cause bone softening, atrophy and even deformation, and affect the absorption of iron in the blood, causing anemia. In addition, Al 3+ Its toxicity causes damage to the central nervous system and leads to n...

AI Technical Summary

Problems solved by technology

[0003] In recent years, based on the identification of Al 3+ There are many designs of fluorescent probe molecules, for example, Biosens Bioelectron (2015), 68, 749-756; Spectrochim Acta A Mol Biomol Spectrosc (2018), 19, 2257-2262; Sensors and Actuators B: Chemical (2017), 247, 451-460 ; Biosens Bioelectron (2017), 77, 530-536; Sensors and Actuators B: Chemical (2018), 266, 95-105; Anal Chim Acta (2016), 942, 104-111; Tetrahedron Letters (2016), 57 (8), 953- 958; these documents are all for the detection of multiple cations, and have not realized the detection of Al 3+ Specific recognition of Sensors and Actuators B: Chemical (2016), 229, 138-144; Spectrochim Acta A Mol Biomol Spectrosc (2018), 201, 185-192; Analytical Methods (2012), 4 (7), 1906; Sensors and Actuators B: Chemical (2017), 240, 916-925; Journal of Photochemistry and Photobiology A: Chemistry (2017), 332, 101-111; Sensors and Actuators B: Chemical (2017), 238, 128-137; Sensors and Actuators B: Chemical (2018), 264,304-311; although these literatures can specifically identify Al 3+ , but the emission wavelength is short and cannot be detected in the long-wavelength range. It is easy to cause light damage to cells or biological samples, and to a certain extent causes background fluorescence self-interference, and most cells have poor permeability, so they cannot be used to identify Al in cells. 3+ , therefore, the design and synthesis of better Al 3+ Fluorescent probes are important

Images