A method for preparing, extracting and purifying threonine
A technology of threonine and seeds, which is applied in the direction of chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of poor stability of mixed fermentation process parameters, increased strain cultivation process, unfavorable industrial production, etc. Achieve the effects of enhanced extrusion, reduced mass transfer restrictions, and avoiding investment
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Embodiment 1
[0024] A method for preparing, extracting and purifying threonine, comprising the steps of:
[0025] Escherichia coli KCCM-10132 seed liquid (1 × 10 9 cfu / ml) was inoculated into the fermenter with an inoculum amount of 8% (volume ratio), and the fermentation time was 72 hours; from the first hour to the 70th hour, the temperature was controlled at 32°C under normal pressure, and Triton was added at the 60th hour X-100 (Triton X-100), the addition amount is 1 μg / ml; at the 71st to 72th hour, the temperature is controlled at 37°C, and the pressure is 2 atmospheres; during the fermentation process, the pH is controlled at 6.8 by automatic feeding of ammonia water; and The residual sugar is controlled at not less than 1.0% by feeding a glucose solution with a concentration of 100g / L, and the fermentation is stopped for 72 hours to obtain a fermented liquid; the culture medium components of the fermenter are: glucose 60g / L, corn steep liquor 15g / L L, ammonium sulfate 5g / L, FeSO ...
Embodiment 2
[0030] A method for preparing, extracting and purifying threonine, comprising the steps of:
[0031] Escherichia coli DSM 14150 seed solution (1 × 10 9 cfu / ml) was inoculated into the fermenter with 8% inoculum, and the fermentation time was 72 hours; the first to 70th hour, the temperature was controlled at 32°C and normal pressure, and at the 60th hour, Triton X-100 ( Triton X-100), the addition amount is 2μg / ml; at 71-72 hours, the temperature is controlled at 38°C, and the pressure is 3 atmospheres; during the fermentation process, the pH is controlled at 6.8 by automatic feeding of ammonia water; and the feeding concentration Control the residual sugar at not less than 1.0% in a glucose solution of 100g / L, and ferment until 72h to stop to obtain a fermentation liquid; the medium components of the fermenter are: glucose 60g / L, corn steep liquor 15g / L, ammonium sulfate 5g / L, FeSO 4 0.02g / L, MgSO 4 0.02g / L, KH 2 PO 4 0.01g / L, K 2 HPO 4 0.01g / L, pH 6.8;
[0032] Use ...
Embodiment 3
[0035] Effects of various factors in the fermentation process on the yield of threonine fermentation:
[0036]Set up a control group, wherein, control group 1: do not change the temperature and pressure, maintain the temperature at 32 ° C and under normal pressure conditions, the rest are the same as in Example 1; control group 2: do not add Triton X-100, the rest are the same as in the embodiment 1; Control group 3: No change in temperature and pressure, no addition of Triton X-100, the rest are the same as in Example 1; the test group is Example 1. The yield of threonine in the fermentation broth of each group is shown in Table 1:
[0037] Table 1
[0038] group Control group 1 Control group 2 Control group 3 test group Threonine production (g / L) 9.3 10.1 7.6 12.9
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