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A method for preparing, extracting and purifying threonine

A technology of threonine and seeds, which is applied in the direction of chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of poor stability of mixed fermentation process parameters, increased strain cultivation process, unfavorable industrial production, etc. Achieve the effects of enhanced extrusion, reduced mass transfer restrictions, and avoiding investment

Active Publication Date: 2021-12-28
内蒙古阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the process of amino acid secretion started late, and people have been focusing on the transporter of amino acid in the cell membrane
In view of the above defects, the applicant's previous patented technology "a method for producing threonine with ultra-low moisture content" adopts a mixed fermentation method, which improves the fermentation efficiency, but the stability of the mixed fermentation process parameters is poor, which is not conducive to industrial production. And increase the strain culture process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for preparing, extracting and purifying threonine, comprising the steps of:

[0025] Escherichia coli KCCM-10132 seed liquid (1 × 10 9 cfu / ml) was inoculated into the fermenter with an inoculum amount of 8% (volume ratio), and the fermentation time was 72 hours; from the first hour to the 70th hour, the temperature was controlled at 32°C under normal pressure, and Triton was added at the 60th hour X-100 (Triton X-100), the addition amount is 1 μg / ml; at the 71st to 72th hour, the temperature is controlled at 37°C, and the pressure is 2 atmospheres; during the fermentation process, the pH is controlled at 6.8 by automatic feeding of ammonia water; and The residual sugar is controlled at not less than 1.0% by feeding a glucose solution with a concentration of 100g / L, and the fermentation is stopped for 72 hours to obtain a fermented liquid; the culture medium components of the fermenter are: glucose 60g / L, corn steep liquor 15g / L L, ammonium sulfate 5g / L, FeSO ...

Embodiment 2

[0030] A method for preparing, extracting and purifying threonine, comprising the steps of:

[0031] Escherichia coli DSM 14150 seed solution (1 × 10 9 cfu / ml) was inoculated into the fermenter with 8% inoculum, and the fermentation time was 72 hours; the first to 70th hour, the temperature was controlled at 32°C and normal pressure, and at the 60th hour, Triton X-100 ( Triton X-100), the addition amount is 2μg / ml; at 71-72 hours, the temperature is controlled at 38°C, and the pressure is 3 atmospheres; during the fermentation process, the pH is controlled at 6.8 by automatic feeding of ammonia water; and the feeding concentration Control the residual sugar at not less than 1.0% in a glucose solution of 100g / L, and ferment until 72h to stop to obtain a fermentation liquid; the medium components of the fermenter are: glucose 60g / L, corn steep liquor 15g / L, ammonium sulfate 5g / L, FeSO 4 0.02g / L, MgSO 4 0.02g / L, KH 2 PO 4 0.01g / L, K 2 HPO 4 0.01g / L, pH 6.8;

[0032] Use ...

Embodiment 3

[0035] Effects of various factors in the fermentation process on the yield of threonine fermentation:

[0036]Set up a control group, wherein, control group 1: do not change the temperature and pressure, maintain the temperature at 32 ° C and under normal pressure conditions, the rest are the same as in Example 1; control group 2: do not add Triton X-100, the rest are the same as in the embodiment 1; Control group 3: No change in temperature and pressure, no addition of Triton X-100, the rest are the same as in Example 1; the test group is Example 1. The yield of threonine in the fermentation broth of each group is shown in Table 1:

[0037] Table 1

[0038] group Control group 1 Control group 2 Control group 3 test group Threonine production (g / L) 9.3 10.1 7.6 12.9

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PUM

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Abstract

The invention belongs to the technical field of biological fermentation and discloses a method for preparing, extracting and purifying threonine, which comprises the following steps: 1) fermentation, 2) centrifugation, 3) microfiltration and ultrafiltration, 4) concentration, isoelectric and crystallization. The process of the invention is simple and feasible, the threonine fermentation efficiency, yield and purity are high, and the application prospect is broad.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for preparing, extracting and purifying threonine. Background technique [0002] Threonine (Threonine, abbreviated as Thr), scientific name 2-amino-3-hydroxybutyric acid, belongs to aliphatic amino acid, slightly sweet, named for its structure similar to threose, is a kind of human, animal and plant protein Essential amino acids are mainly used in medicine, chemical reagents, and nutritional enhancers. They can strengthen dairy products, restore human fatigue, and promote growth and development. In recent years, with the development of the economy, the market demand for threonine has continued to grow steadily, and it is one of the amino acid varieties with the fastest growing demand. Especially, the use in chemistry and biochemistry, food additives, feed additives, etc. has grown rapidly. It replaces tryptophan and becomes the third fastest-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/08C07C227/40C07C229/22C12R1/19
CPCC12P13/08C07C227/40C07C229/22
Inventor 李晓永伏广好徐淑伟徐亚娟宁晓斌王彦妮刘苏宜张建国
Owner 内蒙古阜丰生物科技有限公司
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