Carbon nano material modified electrode capable of simultaneously detecting Cu+ and pH and preparation method

A carbon nanomaterial and electrode modification technology, applied in the field of analysis, can solve problems such as no electrochemical method, and achieve the effects of real-time online electrochemical detection, feasible analysis method and low cost

Active Publication Date: 2019-01-04
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, there is no effective electrochemical method so far, which can simultaneously and accurately treat Cu + and pH detection

Method used

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  • Carbon nano material modified electrode capable of simultaneously detecting Cu+ and pH and preparation method
  • Carbon nano material modified electrode capable of simultaneously detecting Cu+ and pH and preparation method
  • Carbon nano material modified electrode capable of simultaneously detecting Cu+ and pH and preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Utilize CHI660 electrochemical workstation to carry out linear voltammetry scanning to the carbon nanomaterial modified electrode prepared in embodiment 1, scanning speed: 0.05V / s, such as figure 1 Shown; where, a is the linear scanning oxidation curve of bare carbon nanomaterials (bare carbon fiber electrodes) in artificial cerebrospinal fluid, b is the linear scanning oxidation curve of electrodes modified by pH-responsive molecule 1.2-anthraquinone molecules in artificial cerebrospinal fluid, c is the linear scanning oxidation curve of the reference molecule ABTS modified electrode in artificial cerebrospinal fluid, d is the pH response molecule 1,2-anthraquinone, Cu + Recognition ligand (in formula I, R is ) Linear scanning oxidation curves of electrodes co-modified with C and reference molecule ABTS in artificial cerebrospinal fluid. Among them, bare carbon nanomaterials only have charging background current ( figure 1 a). The linear scan oxidation curve of the...

Embodiment 2

[0038] Utilize CHI660 electrochemical workstation to carry out linear voltammetry scanning to the carbon nanomaterial modified electrode prepared in embodiment 1, scanning speed: 0.05V / s, experimental result is as follows figure 2 As shown, curve a is the artificial cerebrospinal fluid added 5μM Cu + The linear scanning curve of the post-carbon nanomaterial modified electrode shows that an oxidation peak appears around 0.3V, and is well separated from the oxidation peak signals of pH-responsive molecules and reference molecules. Curve b is the linear sweep voltammetry curve of the carbon nanomaterial modified electrode after adding 20 μM alkaline EDTA on the basis of a. It can be clearly seen that after adding EDTA to the artificial cerebrospinal fluid, Cu at 0.3V + The oxidation peak of the disappearance, proving that the complexing force of EDTA is stronger than that of selective Cu + Recognition ligand (in formula (I), R group is ), Cu + Released again from the electro...

Embodiment 3

[0040] Utilize CHI660 electrochemical workstation to carry out monovalent copper ion Cu to the carbon nanomaterial modified electrode prepared in embodiment 1 + Titration experiment, and then sequentially carry out linear voltammetry scanning, scanning speed: 0.05V / s, the experimental results are as follows image 3 As shown, the carbon nanomaterial modified electrode is for Cu + There are good responses. Cu trapped on the electrode surface by the recognized ligand + There is an oxidation peak at 0.3V, and the peak current density of the oxidation peak gradually increases with the increase of copper ion concentration, and shows good linearity in the range of 0.5 μM to 11.0 μM. At the same time as the measurement, the signals of the pH probe molecule and the reference molecule are stable and will not interfere with the detection of copper ions.

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Abstract

The invention discloses a carbon nano material modified electrode and a preparation method thereof. A carbon nano material, a Cu+ recognition ligand, a reference molecule and a pH response molecule are used as raw materials, and in a solvent at 10-25 DEG C, the carbon nano material, the Cu+ recognition ligand, the reference molecule and the pH response molecule are modified on the carbon nano material to prepare the carbon nano material modified electrode. The invention also discloses the application of the carbon nano material modified electrode for simultaneous detection of Cu+ and pH in vivo and in vitro. The invention also discloses the application of the carbon nano material modified electrode in simultaneously detecting Cu+ and pH, and the carbon nano material modified electrode hasthe advantages of high selectivity and high sensitivity; the electrode is used for measuring metal ions (Cu+) and pH in a body, and achieves simultaneous, in situ and real-time online electrochemicaldetection. The carbon nano material modified electrode has important significance for further elucidating the role of the Cu+ and pH in physiological and pathological processes.

Description

technical field [0001] The invention belongs to the technical field of analysis, and relates to a novel carbon nanomaterial modified electrode capable of simultaneously detecting cuprous ions and pH in vivo and / or in vitro. Background technique [0002] Copper metal ions (Cu + and Cu 2+ ) are indispensable trace elements for human health, they play an important role in the control of biological metabolism, growth and the development of the immune system. If the content of copper ions in the organism is too high, it will have a toxic effect on the biological system, and even lead to neurological diseases, such as: Alzheimer's disease, Huntington's disease, etc., and the oxidative stress process caused by excessive copper is considered to be the cause of important factor in Alzheimer's disease. There are two states of copper ions in organisms, oxidized Cu 2+ and reducing Cu + . Most antioxidants in the body such as ascorbic acid and glutathione can reduce Cu 2+ into Cu ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48C07C319/20C07C323/42
CPCC07C319/20G01N27/302G01N27/308G01N27/48C07C323/42
Inventor 田阳张立敏刘炜
Owner EAST CHINA NORMAL UNIV
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