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In-situ restoration method for large water area pollution based on microbial strains

A microbial strain, in-situ remediation technology, applied in the field of environmental remediation, can solve problems such as high cost and insufficient effect, and achieve the effect of low cost, water quality improvement, and ecological environment maintenance.

Inactive Publication Date: 2019-01-11
四川晴川环境治理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have alleviated the problem of the deterioration of the river gushing water environment to a certain extent, there are many shortcomings, such as high cost and insufficient effect

Method used

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  • In-situ restoration method for large water area pollution based on microbial strains
  • In-situ restoration method for large water area pollution based on microbial strains

Examples

Experimental program
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Effect test

preparation example Construction

[0019] S1: Putting the first mixed strain in the polluted waters; wherein, the preparation method of the first mixed strain includes the steps of: separately expanding the culture of Synechocystis, lactis and actinomycetes until the cell concentration is 10 8 ~10 9 cfu / g, and then mixed evenly at the ratio of viable bacteria number (15-20): 1: (2-3) to obtain the first mixed strain;

[0020] Preferably, it also includes the steps of sequentially subjecting the uniformly mixed mixture to ultraviolet mutagenesis, chemical mutagenesis and ultraviolet mutagenesis: the irradiation intensity of the ultraviolet lamp used for the ultraviolet mutagenesis is 25-28W, and the ultraviolet lamp and the uniformly mixed mixture The vertical distance is 30-35cm, and the time of ultraviolet irradiation is 20-28s; the chemical mutagen used in the chemical mutagenesis is diethyl sulfate aqueous solution, and the mass percentage of diethyl sulfate aqueous solution is 1.0%-1.2%; The volume ratio o...

Embodiment 1

[0026] This embodiment provides a method for in-situ remediation of large water pollution based on microbial strains, including steps:

[0027] S1: Putting the first mixed strain in the polluted waters; the preparation method of the first mixed strain includes the steps of: separately expanding the cultivation of Synechocystis sp. 8 cfu / g, and then mixed evenly at a ratio of viable bacteria number of 18:1:2.5 to obtain the first mixed strain;

[0028] S2: 24 hours after putting in the first mixed strain, put the second mixed strain in the polluted waters; the preparation method of the second mixed strain includes the steps of: making Synechocystis, denitrifying bacteria, Streptomyces jingyang and Thiobacillus oxidans Expand the culture to a cell concentration of 5×10 8 cfu / g, and then mixed evenly at the ratio of viable bacteria number of 12:1:11:2.5 to obtain the second mixed strain;

[0029] S3: 12 hours after putting in the second mixed strain, put the third mixed strain ...

Embodiment 2

[0032] This embodiment provides a method for in-situ remediation of large water pollution based on microbial strains, including steps:

[0033] S1: Putting the first mixed strains in the polluted waters; wherein, the preparation method of the first mixed strains includes the steps of: separately expanding the cultivation of Synechocystis sp. 8cfu / g, and then mix uniformly at a ratio of viable bacteria number of 18:1:2.5, and then carry out ultraviolet mutagenesis, chemical mutagenesis and ultraviolet mutagenesis on the uniformly mixed mixture to obtain the first mixed strain; wherein, ultraviolet mutagenesis The irradiation intensity of the ultraviolet lamp adopted for mutagenesis is 26W, the vertical distance between the ultraviolet lamp and the mixture after uniform mixing is 33cm, and the time of ultraviolet irradiation is 24s; The mass percentage of ethyl ester aqueous solution is 1.1%; the volume ratio of the uniformly mixed mixture and the chemical mutagen is 100:1.3; th...

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Abstract

The invention relates to an in-situ restoration method for large water area pollution based on microbial strains. The preparation method comprises the following steps: placing first mixed bacterial strain in a polluted water area; wherein the first mixed bacterial strain comprises synechocystis, lactic streptococci and actinomyces; after 20 to 30 hours, placing second mixed bacterial strain in thepolluted water area; wherein the second mixed bacterial strain comprises synechocystis, denitrifying bacteria, streptomyces jingyangensis and thiobacillus oxidans; after 10 to 15 hours, placing a third mixed bacterial strain in the polluted water area; wherein the third mixed bacterial strain comprises synechocystis, rhodopseudomonas palustris, alcaligenes and aspergillus oryzae; and salvaging and collecting flocculates on the surface of polluted water area to purify the water in the water area. The in-situ restoration method can rapidly degrade pollutants such as nitrogen and phosphorus in the polluted water area, can rapidly decompose and convert toxic and harmful substances such as ammonia, nitrite and hydrogen sulfide in the water, and quickly restore the ecological balance and self-purification function of the water area.

Description

technical field [0001] The invention relates to the technical field of environmental remediation, in particular to an in-situ remediation method for large water area pollution based on microbial strains. Background technique [0002] The water purification of lakes, ponds and other waters is a very difficult and complex system engineering, especially when the pollutants in the water cannot be completely separated from the water, on the one hand, it will directly affect the transparency of the water, and then hinder the aquatic organisms in the water and the photosynthesis of plants, affecting the healthy growth of aquatic organisms, microorganisms, plants, etc., and ultimately destroying the ecological balance of the water body; At the same time, with the gradual accumulation of sinking dirt, the water depth will be affected, the original water ecological environment will be changed, the water body will completely lose its use function, and the landscape, human life and heal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/16C02F101/10C02F101/30C02F101/22
CPCC02F1/32C02F3/34C02F2101/101C02F2101/105C02F2101/16C02F2101/22C02F2101/30
Inventor 赖良友
Owner 四川晴川环境治理有限公司
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