A mixed fermentation bacterial agent for soybean products and a two-stage fermentation method
A technology of mixed fermentation bacteria and fermentation method, which is applied in the field of mixed fermentation bacteria agent and two-stage fermentation of soy products, which can solve the problems of inconsistent microbial growth status, general utilization rate of soy products, and unstable fermentation process, etc., reaching the scope of use wide, easy to obtain, and the effect of increasing the content of nutrients
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Embodiment 1
[0028] Design a mixed fermentation agent for soybean products, the mixed fermentation agent includes Mucor racemosus, Rhizopus and Aspergillus oryzae, and the colony-forming unit ratio of Mucor racemosa, Rhizopus and Aspergillus oryzae is 1:1 :1.
[0029] The preparation method of fermentation bacterial agent is:
[0030] Inoculate Mucor racemosus on PDA solid medium, culture at 25°C for 3 days, and collect mycelium; inoculate Rhizopus on PDA solid medium, culture at 25°C for 3 days, and collect mycelium; Aspergillus oryzae was inoculated on PDA solid medium, cultured at 25°C for 3 days, and mycelium was collected.
[0031] The PDA medium composition is: 1.0L of potato extract, 20.0g of glucose, 15.0g of agar, natural pH, potato extract: take 200g of peeled potatoes, cut into small pieces, add 1.0L of water to boil for 30min, filter out the potato pieces, The filtrate was made up to 1.0 L.
[0032] Then the activated mucormyces racemosa, rhizopus and aspergillus oryzae desc...
Embodiment 2
[0034] A two-stage fermentation method in which a mixed fermentation agent is applied to Laba beans. Select soybeans suitable for Laba beans, soak them at room temperature for 20 hours in March, remove the water, put them in a pot and steam them until they are cooled. Cooked and cooled soybeans, spray 100ml of Mucor racemosa liquid, and ferment at a temperature of 20°C; then inoculate 100ml of Rhizopus and Aspergillus oryzae, and ferment at 25°C; add 14% salt, 1% Mix rice wine well, fill it to 4 / 5 of the pottery pot, seal the pot mouth and ferment for 10 days, take it out from the pot, mix with proper amount of vegetable oil, sugar, ginger, and garlic to get Laba beans.
[0035] The difference between the above operations is that soak for 15 hours at room temperature in June, ferment at 20°C, mix well with 15% salt and 1% rice wine, and fill it to 2 / 3 of the volume of the pottery pot. Seal the pot Ferment for 9 days after mouth; soak for 18 hours at room temperature in October...
Embodiment 3
[0038] A two-stage fermentation method in which a mixed fermentation bacterial agent is applied to broad beans. Select broad beans, soak them, peel them, place them in a pot for steaming, and cool them; take 100kg of broad beans and spray total Ferment 100ml of mucormycetes fungus at 20°C until the mold grows to 1.52cm; spray 100ml of rhizopus and aspergillus oryzae and carry out post-fermentation at 25°C, and ferment to obtain fermented broad beans.
[0039] It has been proved by experiments that, compared with broad beans fermented with sterile water, the GABA content after the two-stage fermentation of the mixed bacterial agent is higher than that of the broad beans fermented by the two-stage fermentation using the above-mentioned mixed bacterial agent fermentation method. 31 times, the content of soybean isoflavone functional components after the two-stage fermentation of the mixed bacterial agent is 10 times that of the sterile water fermentation, and the calcium content a...
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