A mixed fermentation bacterial agent for soybean products and a two-stage fermentation method

A technology of mixed fermentation bacteria and fermentation method, which is applied in the field of mixed fermentation bacteria agent and two-stage fermentation of soy products, which can solve the problems of inconsistent microbial growth status, general utilization rate of soy products, and unstable fermentation process, etc., reaching the scope of use wide, easy to obtain, and the effect of increasing the content of nutrients

Active Publication Date: 2019-01-11
湖北楚大爷食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] This kind of bean product is made of soybeans fermented with Mucormyces racemosa, Aspergillus oryzae and Rhizopus to make Laba beans. Laba beans are made by two-stage fermentation method. The growth environment is not sensitive, and the growth cycle is long; Rhizopus and Aspergillus oryzae are more sensitive to growth temperature, growth is relatively slow, and the utilization rate of soybean products is average; if mixed fermentation, the growth state of microorganisms is inconsistent, and the fermentation process is unstable. Soy products are sticky, resulting in poor flavor and easy to cause the growth of bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Design a mixed fermentation agent for soybean products, the mixed fermentation agent includes Mucor racemosus, Rhizopus and Aspergillus oryzae, and the colony-forming unit ratio of Mucor racemosa, Rhizopus and Aspergillus oryzae is 1:1 :1.

[0029] The preparation method of fermentation bacterial agent is:

[0030] Inoculate Mucor racemosus on PDA solid medium, culture at 25°C for 3 days, and collect mycelium; inoculate Rhizopus on PDA solid medium, culture at 25°C for 3 days, and collect mycelium; Aspergillus oryzae was inoculated on PDA solid medium, cultured at 25°C for 3 days, and mycelium was collected.

[0031] The PDA medium composition is: 1.0L of potato extract, 20.0g of glucose, 15.0g of agar, natural pH, potato extract: take 200g of peeled potatoes, cut into small pieces, add 1.0L of water to boil for 30min, filter out the potato pieces, The filtrate was made up to 1.0 L.

[0032] Then the activated mucormyces racemosa, rhizopus and aspergillus oryzae desc...

Embodiment 2

[0034] A two-stage fermentation method in which a mixed fermentation agent is applied to Laba beans. Select soybeans suitable for Laba beans, soak them at room temperature for 20 hours in March, remove the water, put them in a pot and steam them until they are cooled. Cooked and cooled soybeans, spray 100ml of Mucor racemosa liquid, and ferment at a temperature of 20°C; then inoculate 100ml of Rhizopus and Aspergillus oryzae, and ferment at 25°C; add 14% salt, 1% Mix rice wine well, fill it to 4 / 5 of the pottery pot, seal the pot mouth and ferment for 10 days, take it out from the pot, mix with proper amount of vegetable oil, sugar, ginger, and garlic to get Laba beans.

[0035] The difference between the above operations is that soak for 15 hours at room temperature in June, ferment at 20°C, mix well with 15% salt and 1% rice wine, and fill it to 2 / 3 of the volume of the pottery pot. Seal the pot Ferment for 9 days after mouth; soak for 18 hours at room temperature in October...

Embodiment 3

[0038] A two-stage fermentation method in which a mixed fermentation bacterial agent is applied to broad beans. Select broad beans, soak them, peel them, place them in a pot for steaming, and cool them; take 100kg of broad beans and spray total Ferment 100ml of mucormycetes fungus at 20°C until the mold grows to 1.52cm; spray 100ml of rhizopus and aspergillus oryzae and carry out post-fermentation at 25°C, and ferment to obtain fermented broad beans.

[0039] It has been proved by experiments that, compared with broad beans fermented with sterile water, the GABA content after the two-stage fermentation of the mixed bacterial agent is higher than that of the broad beans fermented by the two-stage fermentation using the above-mentioned mixed bacterial agent fermentation method. 31 times, the content of soybean isoflavone functional components after the two-stage fermentation of the mixed bacterial agent is 10 times that of the sterile water fermentation, and the calcium content a...

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PUM

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Abstract

The invention relates to the fermentation field of soybean products, in particular to a mixed fermentation bacterial agent for soybean products and a two-stage fermentation method. The raw materials are: Mucor racemosa, Rhizopus and Aspergillus oryzae, the mucor racemosa, The ratio of Rhizopus to Aspergillus oryzae colony forming units was 1: 1: 1, that mixed bacterial agent is use for fermentingby a two-stage method, As that mucor racemosa is adopt as the dominant strain, A two-stage fermentation method for inoculating Rhizopus and Aspergillus oryzae the active ingredient in soybeans can bepreferentially used, and converted into better-utilized ingredients for use by Rhizopus and Aspergillus oryzae, the growth vigor of the strain is improved, the invention realizes the efficient utilization of protein ingredients in soybean products at the initial fermentation stage of the soybean products, When the inoculum reaches a certain amount, the growth of other miscellaneous bacteria in thefermentation system can be obviously prevented. Therefore, the multi-strain fermentation can significantly enhance the ability of anti-miscellaneous bacteria, effectively avoid sticking of soybean products, and improve the flavor of soybean products.

Description

technical field [0001] The invention relates to the field of fermentation of soybean products, in particular to a mixed fermentation bacterial agent of soybean products and a two-stage fermentation method. Background technique [0002] Soy products are foods made from beans such as soybeans, adzuki beans, mung beans, peas, and broad beans as the main raw materials. Widely spread soy products, such as tofu, shredded tofu, fermented bean curd, soy milk, tempeh, soy sauce, bean sprouts, bean intestines, bean gluten, bean fish, shredded sheep tripe, cat ears, vegetarian chicken wings, soybean ears, Laba beans, etc.; The food made by processing soybean products with microorganisms during fermentation is widely loved by people because of its long storage time and unique taste. [0003] Patent No. CN200610019225.7 proposes a method for brewing Laba beans with multiple strains. The specific steps are as follows: select soybeans, soak them, steam them in a steamer, cool them, spray...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23L11/00C12R1/69C12R1/845C12R1/785A23L11/50
CPCC12N1/14A23L11/50
Inventor 黄友堂肖千红刘研研
Owner 湖北楚大爷食品有限公司
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