A kind of electrochemiluminescent sensor for detecting bisphenol A and its preparation method and application
An electrochemistry and sensor technology, applied in the fields of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis by chemical reactions of materials, etc., can solve the problems of low sensitivity, insufficient luminescence intensity and stability, and achieve high Sensitive detection, low material price, good linear correlation effect
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specific Embodiment 1
[0038] An electrochemiluminescent sensor for detecting bisphenol A, including a working electrode, the working electrode includes a substrate, a composite layer and a modified layer, the substrate is a glassy carbon conductive material, and the composite layer is uniformly dispersed on the The magnetic Fe on the substrate surface of 3 o 4 Nano crystals, the modified layer is composed of perfluorosulfonic acid modified on the surface of the composite layer, and the alkaline luminol solution is used as the electrolyte to construct an electrochemiluminescent sensor for detecting bisphenol A. The preparation method is as follows: figure 1 shown, including the following steps:
[0039] (1) Fe 3 o 4 nanocrystals (Fe 3 o 4 -NCs) preparation
[0040] A. Preparation of iron oleate precursor solution: weigh 2.53g of FeCl 3 ·6H 2 O was dissolved in 25mL of secondary water; 8.86mL of oleic acid was dissolved in 32mL of ethanol; the above two solutions were mixed and added to a th...
specific Embodiment 2
[0050] Optimization of Electrochemiluminescent System
[0051] 1. The effect of the amount of luminol added on the intensity of electrochemiluminescence
[0052] As the precursor of electrochemiluminescence, the concentration of luminol is very important to the influence of the intensity of electrochemiluminescence in the detection system. If the concentration of luminol is too low, it will affect the generation of excited state luminol, thereby affecting the efficiency of electrochemiluminescence; if the concentration of luminol is too high, it will affect the stability and sensitivity of the electrochemiluminescence sensor, and it is not conducive to Amplification of the electrochemiluminescence signal value. From Figure 8 The effect of luminol concentration on ECL intensity can be seen when the addition of luminol solution (5.0×10-5 M in 0.01 M NaOH) was increased from 0.0 to 160.0 μL , the electrochemiluminescence signal value of the system increased rapidly, and reach...
specific Embodiment 3
[0058] The method for detecting the concentration of bisphenol A by using the electrochemiluminescence sensor for detecting bisphenol A prepared under the optimized conditions of the above-mentioned specific example 1 and specific example 2, the specific steps are as follows: the electrochemiluminescence sensor is used as the working electrode , the platinum wire electrode is the auxiliary electrode (counter electrode), and the Ag / AgCl (KCl saturated solution saturated with AgCl) is the reference electrode. Right above the photomultiplier tube. Cyclic voltammetry (CV) was used in the experiment, the negative high voltage of the photomultiplier tube was set to 500V, the test voltage range was 0~+1.8V (vs. Ag / AgCl), and the scan rate was 100mV / s. The test is carried out at room temperature. Before the test, take 2mL of ultrapure water in the electrolytic cell, adjust the pH of the electrochemiluminescence system to 11.0, and add 160 microliters to the electrochemiluminescence sy...
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