Artificial pollination tissue culture technique and method for cultivation and propagation of Qinling wild dendrobium nobile

A technology of artificial pollination of Dendrobium nobile, applied in cultivation, soilless cultivation, horticultural methods, etc., can solve the problems of consumption of Dendrobium seedlings, low survival rate of Dendrobium, and inability to effectively expand the planting area, so as to shorten the time for tissue culture and cost, good social and economic benefits, with the effect of heat preservation and fertility

Inactive Publication Date: 2019-01-18
翟玉军
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the scarcity of wild resources, artificial cultivation techniques have not yet been popularized. In terms of planting methods, most drug farmers continue to use traditional methods, using seedlings for ramet cultivation and cutting cultivation, and consume a large amount of Dendrobium seedlings, resulting in scarcity of germplasm res

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] 1. Artificial pollination production technology of Dendrobium nobile:

[0030] After the wild Dendrobium nobile is harvested, it is placed in the compost of mixed stone pine bark and sawdust for artificial cultivation. When it blooms by itself, it is artificially pollinated. In nature, Dendrobium nobile only blooms but does not bear fruit. Only by artificial pollination can it form capsule.

[0031] 2. Artificial tissue culture technology of Dendrobium nobile

[0032] A. Fresh uncracked fruit or stored seeds are sterilized, sowed into ms basic medium MS+NAA0.25+6-BA3.0mg+IBA0.10mg+pine bark 20% (g / v)+ In the medium of agar 5g / L, carry out seed germination culture, culture temperature 22-25 ℃, light 10--12 hours a day, light intensity 1800-25001x, 15-20 days seed embryos start to germinate, 25-30 days seed The embryo expands and gradually germinates to form a green protocorm;

[0033] B. Protocorm is transferred into ms basic medium+IBA2.5mg+L+BA (6-benzyl adenine) 0....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an artificial pollination tissue culture technique and a method for cultivation and propagation of Qinling wild dendrobium nobile. The method is characterized in that the flowering is accelerated by artificial cultivation of the dendrobium nobile, and the artificial pollination is performed to culture capsules of the dendrobium nobile; by performing the tissue culture production technique and cultivation method on the capsules of the dendrobium nobile, the propagation speed of the seedlings of the dendrobium nobile is increased, the content of effective components is increased, and the seedling exercising and transplanting survival rates are increased; the standard and scale development of the dendrobium nobile is accelerated to realize, and the multiple times of study and test are performed on the special growth habits of the dendrobium nobile according to the use ratio of cytokinin and auxin, screening and usage amount of organic additives, and disinfection methods, so as to obtain the innovative cultivation formula and cultivation method; the seedling exercising and transplanting method of the tissue culture seedling of the dendrobium nobile is solved, and the invention and study on the tissue culture production technique and cultivation method of the dendrobium nobile are completed.

Description

technical field [0001] The patent application of the present invention relates to the technical field of cultivation of traditional Chinese medicinal materials, in particular to artificial pollination of medicinal Dendrobium nobile, capsule tissue culture production technology and cultivation method. Background technique [0002] Dendrobium nobile is the first of the three major species of Dendrobium recorded in "Chinese Pharmacopoeia", and it has been recorded in "Shen Nong Herbal Classic" and "Compendium of Materia Medica". Foping Qinling Dendrobium nobile is used as medicine with stems, which has the effects of reducing fever and preventing convulsions, promoting bone growth, inhibiting tumors, improving human immunity, nourishing yin and clearing heat, lowering blood lipids, and blood pressure. The main raw materials of products such as "Dendrobium Dew", "Dendrobium Beverage" and "Dendrobium Capsule". At present, the supply of wild dendrobium in Qinling Mountains is in ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00A01G31/00A01G24/10A01G24/23A01G24/20A01G24/28
CPCA01H4/001A01G24/10A01G24/20A01G24/23A01G24/28A01G31/00A01H4/005A01H4/008
Inventor 翟玉军
Owner 翟玉军
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap