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Use of active compounds separated from secondary metabolites of Aspergillus versicolor

A secondary metabolite, Aspergillus versicolor technology, applied in the field of microbial drugs, can solve the problems of high difficulty in collecting marine samples and incomplete expression of metabolites, and achieve the effect of convenient operation and reasonable design

Active Publication Date: 2019-01-18
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the collection of marine samples is difficult, and only less than 5% of marine microorganisms have been isolated under routine laboratory conditions, and even the obtained microorganisms cannot fully express all their biosynthetic pathways in the marine environment metabolite

Method used

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  • Use of active compounds separated from secondary metabolites of Aspergillus versicolor
  • Use of active compounds separated from secondary metabolites of Aspergillus versicolor
  • Use of active compounds separated from secondary metabolites of Aspergillus versicolor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The structural formula of the active compound isolated from the secondary metabolites of Aspergillus versicolor is:

[0056] in,

[0057]

[0058] 1. Inhibition of active compounds to α-glucosidase

[0059] Preparation of α-glucosidase solution: Dissolve the lyophilized enzyme powder in 0.1% BSA solution to prepare 100 U / mL enzyme solution, and store it in a refrigerator at -20°C. Before the experiment, pipette a small amount of 100U / mL enzyme solution, first dilute to 20U / mL with 0.1% BSA solution, and then dilute to 0.2U / mL with 0.1% BSA solution for use.

[0060] Preparation of PNPG solution: Weigh a certain amount of PNPG solid, dissolve it with 0.1mol / L phosphate buffered saline (PBS) with a pH of 6.8, and prepare a concentration of 10mmol / L, and then use 1.5mL centrifuge tubes to dispense.

[0061] The activity of α-glucosidase was determined by colorimetry. The active compound to be tested was dissolved in dimethyl sulfoxide (DMSO), and a standby solution...

Embodiment 2

[0071] The use of the active compound isolated from the secondary metabolite of Aspergillus versicolor in the preparation of a medicine for treating diabetes.

[0072] Preparation of tablet: get 40g of active compound, 40g of edible cellulose, 15g of lactose, 0.3g of sodium citrate, 1g of magnesium stearate and an appropriate amount of tablet auxiliaries, mix well, and make tablets according to known tablet making techniques and equipment agent, the product specification is 0.5g / tablet.

Embodiment 3

[0074] The use of the active compound isolated from the secondary metabolite of Aspergillus versicolor in the preparation of a medicine for treating diabetic nephropathy.

[0075] Preparation of granules: Take 38g of active compound, 65g of edible cellulose and appropriate amount of granule auxiliary materials, mix evenly, and use spray drying process for granulation.

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Abstract

The present invention provides the use of active compounds isolated from secondary metabolites of Aspergillus versicolor, The present invention belongs to the technical field of microbial drugs and includes the use in the preparation of drugs for the treatment of diabetes and / or the use in the preparation of drugs for the prevention and / or treatment of diabetic complications and / or the use in thepreparation of drugs as insulin sensitizers. The present invention uses active compound to alpha-glucosidase The inhibitory effect of is better than that of acarbose, which has better effect on prevention and treatment of diabetes, especially type 2 diabetes, and the active compounds come from the secondary metabolites of microorganisms, belonging to natural [alpha]-glucosidase inhibitor, easy tobe absorbed by the body.

Description

technical field [0001] The invention belongs to the technical field of microbial medicines, and in particular relates to the application of active compounds separated from secondary metabolites of Aspergillus versicolor. Background technique [0002] In the nearly one hundred years of research on terrestrial microorganisms, a large number of natural products with diverse chemical structures and significant biological activities have been discovered, which has greatly promoted the development of biotin drugs, such as: penicillin, vancomycin, streptomycin, etc. More and more attention to microorganisms naturally leads to repeated research on microorganisms, repeated development of known compounds, and a lower chance of discovering new compounds. Therefore, people began to pay attention to marine-derived microorganisms with larger microbial reserves and more diverse species. Due to their unique living environment (high pressure, high salt, low temperature, oligotrophic), marin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/085A61P3/10A61P5/50A61P13/12A61P27/02A61P25/00A61P9/00
CPCA61K31/085A61P3/10A61P5/50A61P9/00A61P13/12A61P25/00A61P27/02
Inventor 孙坤来张译文刘文王斌初雪梅陈荫赵玉勤
Owner ZHEJIANG OCEAN UNIV