Method for restoring polluted soil through combination of microorganisms and plants
A technology of combined restoration of polluted soil, applied in the field of soil pollution control, can solve the problems that plants cannot grow biomass, limit the application and development of phytoremediation, and achieve the effect of simple and feasible method, pollution reduction and good repair effect
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Embodiment 1
[0033] A method for joint restoration of contaminated soil by microorganisms and plants, comprising the following steps:
[0034] 1) Mix the magnetotactic bacteria with the soil with a thickness of 20cm on the surface of the polluted soil, and cultivate for 20-25 days. The polluted soil contains at least three heavy metal elements: Pb, Ni, and Cu; the inoculation ratio of the magnetotactic bacteria is per square meter Inoculate 6.5×10 in contaminated soil 10 ; 2) Irrigate the contaminated soil in step 1) to make it in a flooded state, and place a magnet on the contaminated soil to make the magnetotactic bacteria return to the soil surface from the depth of the soil; 3) Collect after 20 days in step 2) and The surface soil in contact with the magnet is rinsed with water, the eluent is collected, and the heavy metals in the soil are extracted. The soil after leaching is returned to the original place and dried until loose; the depth of the collected soil is 10cm; 4) Go to step 3...
Embodiment 2
[0036] A method for joint restoration of contaminated soil by microorganisms and plants, comprising the following steps:
[0037] 1) Mix the magnetotactic bacteria with the soil with a thickness of 20cm on the surface of the polluted soil, and cultivate for 20-25 days. The polluted soil contains at least three heavy metal elements: Pb, Ni, and Cu; the inoculation ratio of the magnetotactic bacteria is per square meter Contaminated soil was inoculated with 7.0×10 10 ; 2) Irrigate the contaminated soil in step 1) to make it in a flooded state, and place a magnet on the contaminated soil to make the magnetotactic bacteria return to the soil surface from the depth of the soil; 3) Collect after 20 days in step 2) and The surface soil contacted by the magnet is rinsed with water, the eluent is collected, and the heavy metals in the soil are extracted. The soil after the rinse is returned to the original place and dried until loose; the depth of the collected soil is 13cm; 4) Go to s...
Embodiment 3
[0039] A method for joint restoration of contaminated soil by microorganisms and plants, comprising the following steps:
[0040] 1) Mix the magnetotactic bacteria with the soil with a thickness of 20cm on the surface of the polluted soil, and cultivate for 20-25 days. The polluted soil contains at least three heavy metal elements: Pb, Ni, and Cu; the inoculation ratio of the magnetotactic bacteria is per square meter Inoculate contaminated soil with 7.5×10 10 ; 2) Irrigate the contaminated soil in step 1) to make it in a flooded state, and place a magnet on the contaminated soil to make the magnetotactic bacteria return to the soil surface from the depth of the soil; 3) Collect after 20 days in step 2) and The surface soil contacted by the magnet is rinsed with water, the eluent is collected, and the heavy metals in the soil are extracted. The soil after the rinse is returned to the original place and dried until loose; the depth of the collected soil is 15cm; 4) Go to step 3...
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