Method for naturally culturing Ganoderma lucidum liquid fermentation and fully utilizing fermentation product
A liquid fermentation and fermentation product technology, applied in the field of biotechnology and food engineering applications, can solve the problems of high recycling costs, waste of resources, low content of active ingredients in fermentation liquid, etc., achieve no environmental pollution, low cost, and improve the economics of production enterprises benefit effect
Examples
Embodiment 1
[0026] 1. Activation of strains: Transfer the preserved ganoderma lucidum slant strains to Potato Dextrose Agar (PDA) slant and culture at 25°C for 7 days.
[0027] 2. Preparation of liquid strains: Inoculate the activated strains into 80 mL Potato Dextrose Broth (PDB) (packed in a 250 mL Erlenmeyer flask); culture at 28°C and 160 rev / min for 4 days, and it is a first-class strain; Ultrasonic crush the primary species, draw 20 mL and inoculate it into 400 mL PDB medium (packed in a 1000 mL Erlenmeyer flask); culture at 28°C and 120 rev / min for 3 days, a large number of dense small mycelial balls are formed, and used as the secondary species for fermentation inoculation;
[0028] 3. Preparation of natural fermentation medium (per liter): Peel the tomatoes and cut them into fine pieces; weigh 200 g of tomatoes and 150 g of bran, put them in 1000 mL of pure water, boil for 30 min, filter and discard the residue, and use Add purified water to 1000 mL, add 40 g sucrose, the form...
Embodiment 2
[0041] 1. The activation of Ganoderma lucidum strain is the same as in Example 1;
[0042] 2. The preparation of the first-level species and the second-level species is the same as in Example 1;
[0043] 3. Preparation of natural fermentation medium; per liter: Peel carrots and cut them into fine pieces; weigh 200 g carrots and 150 grams of bran in 1000 mL of pure water, boil for 30 min, filter and discard the residue, and wash with pure water Supplement to 1000 mL, add 40 g sucrose, the formula is: 200 g / L carrot, 150 g / L bran, 40 g / L sucrose;
[0044] 4. Sterilize 10 L of seed tanks at 121°C for 30 minutes, add 6.65 L of fermentation medium, and sterilize at 121°C for 30 minutes
[0045] After cooling, insert 350 mL of secondary seed, culture at 28°C and 160 rpm for 4 days, and use for inoculation and fermentation;
[0046] 5. Add 47.5 L of fermentation medium into the sterilized 100 L fermenter, and sterilize at 121°C for 30 minutes;
[0047]6. When the fermentation ...
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Description
Claims
Application Information
- IPC
- C12P19/04; C12P1/02; A23L2/38; A23L2/52; A23L2/68; A23L2/72; A23L33/00; C12R1/645
- CPC
- A23L2/382; A23L2/52; A23L2/68; A23L2/72; A23L33/00; C12P1/02; C12P19/04; C12R2001/645
- Inventors
- 刘盛荣; 张维瑞