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Method for uniformly and synchronously adding isotope label solution into soil

A technology of isotope labeling and substance solution, which is applied in the fields of plant nutrition science, environmental science and soil science, can solve the problems that affect the accuracy of labeling, plant growth, change soil aeration, and lack of synchronization, etc., to achieve easy promotion, Ease of operation, improved synchronization and accuracy

Active Publication Date: 2019-01-29
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But this method also has many disadvantages: 1. After the needle of the syringe is pulled out, many pinholes will be left in the soil column, which changes the air permeability of various parts of the soil; 2. When the needle is inserted into the soil, it will inevitably prick On the root system of the plant, it affects the accuracy of the marking and the growth of the plant; 3. Many needles need to be inserted into the same soil column, and there will be a sequence in the marking time, and the synchronization of the same soil marking cannot be achieved; 4. For Deep soil layers beyond the reach of the needle, the method will be difficult to achieve
Its polymer material ensures its stability and biochemical inertness in the soil, and its microporous tube wall ensures that soil particles, colloids and microorganisms will not enter the hollow fiber tube to block and pollute the tube, and at the same time, the soil solution can enter and exit freely pipeline, which no one has yet applied to the process of adding isotopic markers to soil

Method used

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  • Method for uniformly and synchronously adding isotope label solution into soil
  • Method for uniformly and synchronously adding isotope label solution into soil
  • Method for uniformly and synchronously adding isotope label solution into soil

Examples

Experimental program
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Effect test

Embodiment 1

[0020] The pure soil cultivated in the cuboidal potting pots contained 15 N isotopic ammonium nitrate ( 15 NH 4 14 NO 3 ) solution mark: use the horizontal soil column infiltration method to measure the target soil that has passed through the 60 mesh sieve at the water content θ=0.30cm 3 cm -3 The water diffusivity under the culture condition D=0.839cm 2 min -1 , the time required for uniform diffusion is set as t=10min, according to the empirical formula Get the maximum release source spacing d m = 2.388 cm. Such as figure 1 As shown, in a cuboid culture tank whose length, width and height are 15cm×15cm×25cm, a hollow fiber of polyvinyl alcohol with an outer diameter of 0.9mm, an inner diameter of 0.475mm, and a tube wall aperture of 40nm is arranged in a few characters: the first The distance between adjacent hollow fibers in the same plane is 2 cm, and there are 8 hollow fibers arranged in parallel and vertically. The distance between the hollow fibers at both en...

Embodiment 2

[0022] The pure soil cultivated in the cuboidal potting pots contained 15 N isotopic ammonium nitrate ( 15 NH 4 14 NO 3 ) solution mark: the target soil measured through a 60 mesh sieve has a water content of θ=0.35cm 3 cm -3 The water diffusivity under the culture condition D=2.221cm 2 min -1 , the time required for uniform diffusion is set as t=10min, according to the empirical formula Get the maximum release source spacing d m = 3.886 cm. Such as figure 2 As shown, in a cuboid culture tank whose length, width and height are 15cm×15cm×25cm, a hollow fiber of polyvinyl alcohol with an outer diameter of 0.9mm, an inner diameter of 0.475mm, and a tube wall aperture of 40nm is arranged in a few characters: the first The distance between adjacent hollow fibers in the same plane is 3.5 cm, and there are 5 hollow fibers arranged in parallel and vertically. The distance between the hollow fibers at both ends is 0.5 cm from the plane of the adjacent culture tank wall; Tw...

Embodiment 3

[0024] The pure soil cultivated in the cuboidal potting pots contained 15 N isotopic ammonium nitrate ( 15 NH 4 14 NO 3 ) solution marking: the target soil measured through a 60 mesh sieve has a water content of θ=0.40cm 3 cm -3 The water diffusivity under the culture condition D=4.583cm 2 min -1 , the time required for uniform diffusion is set as t=10min, according to the empirical formula Get the maximum release source spacing d m = 5.582 cm. Such as image 3 As shown, in a cuboid culture tank whose length, width and height are 15cm×15cm×25cm, a hollow fiber of polyvinyl alcohol with an outer diameter of 0.9mm, an inner diameter of 0.475mm, and a tube wall aperture of 40nm is arranged in a few characters: the first The distance between adjacent hollow fibers in the same plane is 5.5 cm, and there are 3 hollow fibers arranged in parallel and vertically. The distance between the hollow fibers at both ends is 2.0 cm from the plane of the adjacent culture tank wall; ...

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Abstract

The invention relates to a method for uniformly and synchronously adding isotope label solution into soil. the water content in the culture condition of the soil to be treated is theta; the water diffusion rate of target soil under the water content condition is measured to be D; uniform diffusion is carried out within t time, and the maximum interval dm of a release source can be calculated according to an empirical formula (formula), wherein a is a constant; hollow fibers with a micropore tube wall are embedded in the target soil according to the result, and the distance d between the fibertubes is not greater than dm. According to the technical method in the invention, the cheap hollow fibers are used for uniformly and synchronously adding the isotope label solution into the soil, thereby ensuring the uniformity of the isotope labelling soil experiment, improving the synchronism and accuracy of the labelling process and reducing experimental errors; the application range is wide; the operation is simple and convenient; and the method is easy to promote.

Description

technical field [0001] The invention belongs to the technical fields of soil science, plant nutrition science and environmental science, and in particular relates to a method for uniformly and synchronously adding an isotope marker solution to soil. Background technique [0002] Most elements in nature are composed of two or more isotopes, and the isotopes of each element have a stable relative content in the natural state. Therefore, people can add a certain amount of isotopic markers different from the natural abundance of certain elements or several elements to the soil as tracers, track the whereabouts of these substances and changes in isotopic composition, and study the occurrence of these elements in soil. involved in biogeochemical cycles. Therefore, isotope tracer technology has a wide range of applications in research fields such as agriculture, ecology, environment and life sciences. [0003] How to uniformly label isotope markers in soil is a key technical diff...

Claims

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Application Information

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IPC IPC(8): G01N33/24G01N1/28
CPCG01N1/28G01N33/24G01N33/248G01N33/245
Inventor 李汛段增强
Owner INST OF SOIL SCI CHINESE ACAD OF SCI