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Method for hydrolyzing silkworm cocoons through weak base via microwaves

A silkworm cocoon and microwave technology, applied in the field of silk protein, can solve the problems of simultaneous preparation, protein destruction, and high manufacturing costs, and achieve the effects of improving preparation efficiency and product utilization, reducing the destruction of effective amino acids, and shortening the hydrolysis treatment time.

Active Publication Date: 2019-02-12
YIBIN PINGSHAN HUIRUI OIL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above-mentioned patents can prepare sericin peptide protein or silk fibroin peptide protein well, but because sericin and silk fibroin complement each other, the above-mentioned patents can only prepare one kind of sericin peptide protein or silk fibroin peptide protein, and Can not prepare two kinds at the same time, it has defects such as low preparation efficiency and low product utilization rate
Moreover, both of the above two pieces used strong acid or strong base (sodium hydroxide) in the preparation process. After hydrolysis, it is necessary to neutralize the acid or base of the hydrolyzed liquid and physically extract the silk fibroin sericin peptide protein. Waste liquid treatment and discharge The amount is also increased, and the pollution control of the post-process is increased, and the efficiency of the process will be low if the process is increased.
Therefore, there are defects such as low preparation efficiency, high manufacturing cost, large pollution, and large protein damage, resulting in that the quality of the product cannot be guaranteed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for hydrolyzing silkworm cocoons with a weak base microwave, comprising the steps of:

[0035] (1) Cleaning and drying

[0036] Clean the cocoons first, then heat and dry the cocoons until the drying rate reaches 55%.

[0037] In this step, the impurities on the silkworm cocoons are preferably removed by spraying. After the impurities are removed, the silkworm cocoons are dried in the following manner. The specific drying process includes three stages: preheating, constant-speed drying and deceleration drying. In the preheating stage, a hot air tumbler is used to heat the silkworm cocoons, so that the heat energy penetrates into the cocoon cavity through the cocoon layer to kill the pupae, and the water in the pupae enters the evaporation stage; The evaporation rate on the surface of the cocoon is in a balanced state until the water diffusion rate of the pupal body gradually lags behind the evaporation rate on the surface of the cocoon layer; the deceleration...

Embodiment 2

[0046] A method for hydrolyzing silkworm cocoons with a weak base microwave, comprising the steps of:

[0047] (1) Cleaning and drying

[0048] Firstly, the silkworm cocoons are cleaned and removed, and then the silkworm cocoons are heated and dried until the drying rate is 60%.

[0049] In this step, the impurities on the silkworm cocoons are preferably removed by spraying. After the impurities are removed, the silkworm cocoons are dried in the following manner. The specific drying process includes three stages: preheating, constant-speed drying and deceleration drying. In the preheating stage, a hot air tumbler is used to heat the silkworm cocoons, so that the heat energy penetrates into the cocoon cavity through the cocoon layer to kill the pupae, and the water in the pupae enters the evaporation stage; The evaporation rate on the surface of the cocoon is in a balanced state until the water diffusion rate of the pupal body gradually lags behind the evaporation rate on the...

Embodiment 3

[0058] A method for hydrolyzing silkworm cocoons with a weak base microwave, comprising the steps of:

[0059] (1) Cleaning and drying

[0060] Clean the cocoons first, then heat and dry the cocoons until the drying rate is 58%.

[0061] In this step, the impurities on the silkworm cocoons are preferably removed by spraying. After the impurities are removed, the silkworm cocoons are dried in the following manner. The specific drying process includes three stages: preheating, constant-speed drying and deceleration drying. In the preheating stage, a hot air tumbler is used to heat the silkworm cocoons, so that the heat energy penetrates into the cocoon cavity through the cocoon layer to kill the pupae, and the water in the pupae enters the evaporation stage; The evaporation rate on the surface of the cocoon is in a balanced state until the water diffusion rate of the pupal body gradually lags behind the evaporation rate on the surface of the cocoon layer; the deceleration dryi...

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PUM

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Abstract

The invention discloses a method for hydrolyzing silkworm cocoons through a weak base via microwaves. The method comprises the following steps: (1) washing and drying; (2) cutting and separating; (3)carrying out primary hydrolysis and degumming; adding silkworm cocoon sheets and a weak base mixed solution formed by mixing sodium bicarbonate and sodium chloride into a microwave reactor; hydrolyzing for 110 to 130min under normal pressure and at the temperature of 80 DEG C; after hydrolyzing, obtaining a coarse sericin peptide solution and a silk protein fiber solid; pumping out the coarse sericin peptide solution and carrying out ultra-filtration to obtain sericin peptide protein; (4) carrying out secondary hydrolysis to extract silk fibroin peptide protein; then adding the weak base mixedsolution into the microwave reactor again; then hydrolyzing for 110 to 130min under normal pressure and at the temperature of 100 DEG C; carrying out heat insulation for 8 to 11h to completely hydrolyze the silk protein fiber solid into a coarse silk fibroin solution; carrying out ultra-filtration on the coarse silk fibroin solution to obtain silk fibroin protein. The method disclosed by the invention can be used for effectively preparing the sericin peptide protein and the silk fibroin protein in one step and the aim of improving the preparation efficiency and the utilization rate of products is realized.

Description

technical field [0001] The invention relates to the technical field of silk protein, in particular to a method for hydrolyzing silkworm cocoons with a weak alkali microwave. Background technique [0002] The silk industry originated in my country has a long history of thousands of years. However, the application of silk is mainly to use silk fiber as clothing material. With the progress of society and the development of modern science and technology, the development and utilization of new functions of silk are becoming more and more important. more and more become a trend. [0003] At the end of the 1920s, the silk industry experienced overproduction for the first time in the world. From 1933, people took the lead in applying silk to the field of non-clothing clothing and used it through research. Since then, the multifunctional development and utilization of silk protein has begun. It is found through research that silk is mainly composed of 20-30% sericin peptide protein a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/14C07K1/34C07K1/36B01J19/12
CPCB01J19/126C07K14/43586
Inventor 孙益华
Owner YIBIN PINGSHAN HUIRUI OIL CO LTD
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