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Construction method and quality detection method of uplc characteristic map of hangju medicinal material

A quality detection method and feature map technology, applied in the construction method of UPLC feature map, the field of quality testing, can solve the problems of unable to reflect the quality of Hang chrysanthemum, confusion, unable to reflect the quality of chrysanthemum, etc., to reduce the possibility of human processing, characteristics The peak information is rich and the effect of comprehensive monitoring of product quality

Active Publication Date: 2021-10-29
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aware of this problem, some scholars have compared and evaluated the internal components of different varieties of chrysanthemums from the perspective of multi-index control, but the multi-component analysis brings comprehensive information and at the same time causes confusion.
This status quo cannot reflect the quality of chrysanthemums well, let alone the quality of Hangchrysanthemums.

Method used

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  • Construction method and quality detection method of uplc characteristic map of hangju medicinal material
  • Construction method and quality detection method of uplc characteristic map of hangju medicinal material
  • Construction method and quality detection method of uplc characteristic map of hangju medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Construction of the characteristic map of Chrysanthemum chrysanthemum

[0050] 1. Instruments, reagents, reagents and evaluation software

[0051] 1.1 Instrument

[0052] Thermo ultra-high performance liquid chromatography (Vanquish (DAD), Thermo Fisher); Waters HSS-T 3 Column (100×2.1mm, 1.8μm); Agilent ultra-high performance liquid chromatography (1290Infinity, Agilent Corporation); Mettler XP26 millionth balance (Swiss Mettler Toledo company); KQ5500DE ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); constant temperature water bath (Shanghai Yiheng Technology Co., Ltd., model: HWS28); Milli-Q ultrapure water purification system (Millipore, USA).

[0053] 1.2 Reagents

[0054] Ethanol (Tianjin Fuyu Fine Chemical Co., Ltd., analytically pure); methanol (Tianjin Fuyu Fine Chemical Co., Ltd., analytically pure); phosphoric acid (Tianjin Kemiou Chemical Reagent Co., Ltd., chromatographically pure); acetonitrile (Merck Co., Ltd., chromatograph...

Embodiment 2

[0149] Embodiment 2: A kind of quality detection method of Hangchrysanthemum medicinal material and decoction pieces

[0150] Preparation of the test solution: take 0.4 g of the powder of the chrysanthemum to be tested (S22-S27) (through the No. 1 sieve), accurately weigh it, put it in a stoppered conical flask, and accurately add 70% (v / v) methanol 25 mL of the solution was weighed, heated to reflux for 40 minutes, taken out, allowed to cool, and weighed again, supplemented with 70% (v / v) methanol solution to reduce the lost weight, shake well, filter, and take the filtrate to obtain the final product.

[0151] Preparation of reference solution: same as under "2.2 Preparation of reference solution".

[0152] Chromatographic conditions and system adaptability test: same as under "2.3 Chromatographic conditions".

[0153] Determination method: same as under "2.4 Determination method".

[0154] Establishment of qualified indicators and formulating spectra and judgment method...

Embodiment 3

[0155] Embodiment 3: a kind of quality detection method of chrysanthemum medicinal material

[0156] Preparation of the test solution: Take an appropriate amount of this product powder (batch numbers: S28, S29, passed through a No. 1 sieve), grind it finely, take about 0.4g, accurately weigh it, put it in a stoppered Erlenmeyer flask, and add 70% methanol precisely 25mL, weighed, heated to reflux for 40min, taken out, allowed to cool, weighed again, made up for lost weight with 70% methanol, shaken well, filtered, and obtained the filtrate.

[0157] Preparation of reference solution: same as under "2.2 Preparation of reference solution".

[0158] Chromatographic conditions and system adaptability test: same as under "2.3 Chromatographic conditions".

[0159] Determination method: same as under "2.4 Determination method".

[0160] Judgment method of qualified indicators: tentatively determined that the qualified indicators of the chrysanthemum medicinal material are 10 char...

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Abstract

The invention relates to a construction method and a quality detection method of a UPLC characteristic map of Hang chrysanthemum medicinal materials. The construction method includes: preparation of a test solution: taking the medicinal material of chrysanthemum, pulverizing, extracting with solvent, filtering, and the obtained filtrate is used as a solution for the test; preparation of a reference solution: taking chlorogenic acid, 3,5‑O‑ two coffee Acylquinic acid, 4,5-O-dicaffeoylquinic acid, luteolin, and montiflorin were dissolved in a solvent, and the obtained solution was used as a reference solution; UHPLC detection: absorb the test solution, The reference substance solution was injected into a liquid chromatograph, and detected, and the UPLC characteristic spectrum of the medicinal material of Hang chrysanthemum was obtained. The characteristic peaks of the UPLC characteristic map are rich in information, which can reflect the chemical composition of Hangju medicinal materials, and can also effectively characterize the quality of Hangju decoction pieces and standard decoctions, and identify the authenticity of products.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a construction method and a quality detection method of a UPLC characteristic map of a chrysanthemum medicinal material. Background technique [0002] Chrysanthemum is the dry flower head of Chrysanthemum morifolium Ramat. Chrysanthemum was first recorded in "Shen Nong's Materia Medica" in the Eastern Han Dynasty, and was listed as top grade. It is said: "long-term use can benefit blood qi, make light of body, resist aging, and prolong life". For anemopyretic cold, headache and dizziness, conjunctival congestion and swelling pain, blurred vision, sore carbuncle and swollen toxin. The 2015 edition of the Chinese Pharmacopoeia contains five main medicinal chrysanthemums: "Gongju", "Chuju", "Boju", "Hangju" and "Huaiju". After consulting a large number of literatures, it is found that the types and contents of the main chemical components of chrysanthemums are d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 魏梅陈菊英霍文杰程学仁丁青杨晓东刘晓霞陈向东
Owner GUANGDONG YIFANG PHARMA
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