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Colloidal gold test paper, preparation method thereof and rice seed vigor detecting method

A technology for colloidal gold test paper and rice seeds, which can be applied to measurement devices, biological tests, material inspection products, etc., can solve problems such as unfavorable rapid measurement and complicated operations, and achieve the effects of easy observation, simple steps and high accuracy.

Inactive Publication Date: 2019-02-22
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods require professional instruments and relatively complicated operations, which are not conducive to rapid determination

Method used

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  • Colloidal gold test paper, preparation method thereof and rice seed vigor detecting method
  • Colloidal gold test paper, preparation method thereof and rice seed vigor detecting method
  • Colloidal gold test paper, preparation method thereof and rice seed vigor detecting method

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preparation example Construction

[0055] The embodiment of the present invention discloses a method for preparing a colloidal gold test paper for detecting rice seed vigor, comprising the following steps:

[0056] (S1) Adjust the pH value of the colloidal gold solution to neutral, add the antibody of antioxidative enzyme, continue to stir; add the macromolecular polymer, centrifuge after stirring, take the supernatant and centrifuge again, keep the precipitate, and use the buffer for the precipitate completely dissolved, coated on the colloidal gold binding pad, and dried for use; the antioxidative enzyme antibody is ascorbate peroxidase antibody or manganese superoxide dismutase antibody;

[0057] (S2) coating the embryo protein extract of rice seeds with a germination rate of 99% on the detection line;

[0058] (S3) coating the quality control line with immunoglobulin IgG matched with the anti-oxidase antibody;

[0059] (S4) Adhere the sample pad, the gold standard conjugate pad, the nitrocellulose membrane...

Embodiment 1

[0083] 1. Preparation of colloidal gold solution (trisodium citrate reduction method)

[0084] Accurately measure 100ml of deionized water in a conical flask, add 1ml of 1% (w / v) chloroauric acid aqueous solution, heat to complete boiling, quickly add 1ml of 1% (w / v) trisodium citrate aqueous solution, and continue heating When the solution turns into a stable wine red color, boil for another 10 minutes, stop heating, cool at room temperature, add deionized water to make up to a volume of 100ml to prepare a colloidal gold solution, and store it in the dark at 4°C for later use.

[0085] 2. Pretreatment of Colloidal Gold Bonding Pads

[0086] Soak the cut colloidal gold bonding pad in 50ml gold standard pad treatment solution [2.5g sucrose, 0.5gBSA, 50ml PBS buffer solution (pH 7.4)], heat in a water bath at 37°C for 30min, take it out and place it at 37°C Dry in an oven, and store sealed at room temperature for later use.

[0087] 3. Preparation of gold-labeled antibody

[...

Embodiment 2

[0094] 1. Preparation of colloidal gold solution (trisodium citrate reduction method)

[0095] Accurately measure 100ml of deionized water in a conical flask, add 1ml of 1% (w / v) chloroauric acid aqueous solution, heat to complete boiling, quickly add 1ml of 1% (w / v) trisodium citrate aqueous solution, and continue heating When the solution turns into a stable wine red color, boil for another 10 minutes, stop heating, cool at room temperature, add deionized water to make up to a volume of 100ml to prepare a colloidal gold solution, and store it in the dark at 4°C for later use.

[0096] 2. Pretreatment of Colloidal Gold Bonding Pads

[0097] Soak the cut colloidal gold bonding pad in 50ml gold standard pad treatment solution [2.5g sucrose, 0.5gBSA, 50ml PBS buffer solution (pH 7.4)], heat in a water bath at 37°C for 30min, take it out and place it at 37°C Dry in an oven, and store sealed at room temperature for later use.

[0098] 3. Preparation of gold-labeled antibody

[...

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Abstract

The invention relates to the field of seed vigor detection and in particular relates to a colloidal gold test paper, a preparation method thereof and a rice seed vigor detecting method. The colloidalgold test paper comprises a base plate, a sample pad, a colloidal gold bonding pad, a nitrocellulose membrane and a water absorption pad; the nitrocellulose membrane is provided with a detection lineand a quality control line; the colloidal gold bonding pad is combined with a colloidal gold marker which is an antioxidant enzyme antibody marked by colloidal gold; the detection line is coated withan embryo protein extracting solution of rice seeds having the germination rate of 99%; and the quality control line is coated with an immune globulin IgG matched with the antioxidant enzyme antibody.The colloidal gold test paper disclosed by the invention is capable of rapidly detecting the vigor of the rice seeds, simple in step, high in detection result accuracy rate and capable of easily observing a result.

Description

technical field [0001] The invention relates to the field of detection of seed vigor, in particular to a colloidal gold test paper, a preparation method thereof and a detection method of rice seed vigor. Background technique [0002] Rice is one of the main food crops in my country, and occupies an important share in production and preservation of germplasm resources. Seeds are the most basic means of production in rice production, and their quality directly affects the yield and quality of rice, and seed vigor is an important indicator of seed quality. There are three main methods for the determination of seed vigor: germination method, adversity method and physiological and biochemical method. The traditional method of detecting seed vigor is to conduct a germination test, which is usually repeated three or four times, with 100 seeds per repetition, dark culture in a constant temperature germination box, and the germination potential is obtained after 4 days of cultivatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/68
CPCG01N33/558G01N33/6854
Inventor 尹广鹍章泽森辛霞卢新雄陈晓玲张金梅何娟娟
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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