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A kind of Tongkat Ali polysaccharide with immunomodulatory and anti-erythrocyte hemolytic activity and preparation method thereof

A technology of red blood cell hemolysis and immune regulation, applied in the field of bioengineering, to achieve the effect of ensuring safety, good molecular weight, and retaining related activities

Active Publication Date: 2021-02-12
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant polysaccharides are a class of plant extracts with special physiological activities. So far, systematic studies on Tongkat Ali polysaccharides at home and abroad are rarely reported.

Method used

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  • A kind of Tongkat Ali polysaccharide with immunomodulatory and anti-erythrocyte hemolytic activity and preparation method thereof
  • A kind of Tongkat Ali polysaccharide with immunomodulatory and anti-erythrocyte hemolytic activity and preparation method thereof
  • A kind of Tongkat Ali polysaccharide with immunomodulatory and anti-erythrocyte hemolytic activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A polysaccharide with immunoregulatory activity and anti-erythrocyte hemolytic activity, comprising the steps of:

[0039] (1) Preparation of crude product of Tongkat Ali polysaccharide: Wash and dry the roots of Tongkat Ali tree, crush them and pass through a 40-mesh sieve to obtain the Tongkat Ali pulverized product, and put it in a dry place for later use; condense the above pulverized product with 75% ethanol Reflux, the reflux temperature is 70°C, the reflux time is 2h, remove impurities and basically remove the pigment, discard the supernatant by suction filtration, and dry the filter residue naturally for later use; add water to the filter residue at a ratio of 1:10 (g / ml), and Stir at 70°C for 1h, 6000r / min, centrifuge at 4°C for 10min, collect the supernatant and concentrate, add 5 times the volume of absolute ethanol, let stand at 4°C for 8h, 6000r / min, centrifuge at 4°C for 10min; The obtained precipitate was collected with 3 times the volume of sevage reagen...

Embodiment 2

[0044] A polysaccharide with immunoregulatory activity and anti-erythrocyte hemolytic activity, comprising the steps of:

[0045] (1) Preparation of crude product of Tongkat Ali polysaccharide: Wash and dry the roots of Tongkat Ali tree, crush them and pass through a 60-mesh sieve to obtain the Tongkat Ali pulverized product, and put it in a dry place for later use; condense the above pulverized product with 75% ethanol Reflux, the reflux temperature is 70°C, the reflux time is 2h, remove impurities and basically remove the pigment, discard the supernatant by suction filtration, and dry the filter residue naturally for later use; add water to the filter residue at a ratio of 1:20 (g / ml), and Stir at 80°C for 2h, 8000r / min, centrifuge at 4°C for 15min, collect the supernatant and concentrate, add 8 times the volume of absolute ethanol, let stand at 6°C for 10h, 8000r / min, centrifuge at 4°C for 15min; The obtained precipitate was collected with 4 times the volume of sevage reage...

Embodiment 3

[0049] A polysaccharide with immunoregulatory activity and anti-erythrocyte hemolytic activity, comprising the steps of:

[0050] (1) Preparation of crude polysaccharides of Tongkat Ali: Wash and dry the roots of Tongkat Ali tree, crush them and pass through an 80-mesh sieve to obtain the Tongkat Ali pulverized product, and put it in a dry place for later use; condense the above pulverized product with 75% ethanol Reflux, the reflux temperature is 70°C, the reflux time is 2h, remove impurities and basically remove the pigment, discard the supernatant by suction filtration, and dry the filter residue naturally for later use; add water to the filter residue at a ratio of 1:30 (g / ml), and Stir at 90°C for 3h, centrifuge at 10,000r / min at low temperature for 20min, collect the supernatant and concentrate, add 10 times the volume of absolute ethanol, let stand at 8°C for 12h, centrifuge at 10,000r / min, and 4°C for 20min; the obtained precipitate Use 5 times the volume of sevage rea...

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Abstract

The invention discloses a Tongkat Ali polysaccharide with immune regulation and anti-erythrocyte hemolysis activities and a preparation method thereof. The method is as follows: taking Tongkat Ali root as raw material, extracting Tongkat Ali polysaccharide through water extraction and alcohol precipitation, separating and purifying Tongkat Ali polysaccharide through DEAE-Sepharose anion exchange chromatography and SephadexG50 gel permeation chromatography, the average molecular weight of the polysaccharide is 14.3ku, It is mainly composed of xylose, arabinose, glucuronic acid and galacturonic acid. In addition, the polysaccharide also has a certain anti-erythrocyte hemolysis effect. The polysaccharide promoted the secretion of NO, IL‑6 and TNF‑ α Compared with the blank control, its ability has increased by 13.27, 7.8 and 9.0 times respectively; when its concentration is 2000μg / mL, the red blood cell hemolysis rate is only 9.15%, and it can basically restore the damaged red blood cells to the normal cell shape.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to a polysaccharide of tongkat ali with immune regulation and anti-erythrocyte hemolytic activity and a preparation method thereof. Background technique [0002] Tongkat Ali (Eurycomalongifolia) is a plant of the genus Tongkat Ali in the bitter wood family. It is mainly distributed in Southeast Asian countries. Together with bird's nest and tinware, it is also known as the three major national treasures of Malaysia, and is known as "Malaysian ginseng". As a well-known traditional herbal medicine in Southeast Asia, the whole plant can be used as medicine, but the root is often used as the main medicinal part, which has the effects of aphrodisiac, improvement of male sexual dysfunction, and anti-malarial effects. Due to its special effects, the research on Tongkat Ali, especially the structure and function of the active substances in Tongkat Ali, has been increasing ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P37/02A61P7/00
CPCA61P7/00A61P37/02C08B37/0003C08B37/006
Inventor 吴晖贺萍王倩赖富饶张猛猛
Owner SOUTH CHINA UNIV OF TECH
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