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Stenotrophomonas and application thereof as well as microorganism bacterium agent

A technology of oligotrophomonas and microbial agent, which is applied in the field of environmental microorganisms, can solve the problems of slow proliferation of denitrifying bacteria, difficulty in maintaining high-concentration denitrification, and destruction of the greenhouse effect environment, so as to save additional carbon The effect of low source cost, complete denitrification process, and small environmental pollution

Active Publication Date: 2019-03-01
中原环保股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also some disadvantages in the traditional biological denitrification process: first, the proliferation rate of ordinary denitrifying bacteria is slow, and it is difficult to maintain a high concentration for denitrification; Third, when the process treats sewage with a low C / N ratio, it is necessary to add a large amount of carbon-containing organic matter to ensure the denitrification effect, which increases the cost; fourth, the traditional denitrification reaction is not complete, and the intermediate products NO, N 2 The long-term accumulation of O and other gases aggravates the damage to the environment caused by the greenhouse effect

Method used

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  • Stenotrophomonas and application thereof as well as microorganism bacterium agent
  • Stenotrophomonas and application thereof as well as microorganism bacterium agent
  • Stenotrophomonas and application thereof as well as microorganism bacterium agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 strain screening

[0028] Isolation process of DB strain: Take 1ml of the mixed bottom sludge of a sewage plant pond, connect it to the liquid denitrification medium for long-term anaerobic acclimatization in a high-nitrogen environment, inoculate the medium and seal it with paraffin wax, and keep the anaerobic environment isolated from the air. The screening test is to grow for 6-8 days, and the characteristic test in the later stage is to acclimatize and cultivate in the seed culture solution for 3-4 days, and grow to about OD600=1. After acclimatization, carry out 10-fold gradient dilution with sterile water, take 10 -4 、10 -5 、10 -6 Spread on the solid denitrification identification medium, and do 3 parallels for each dilution until there are clear colonies on the plate, observe the size and color of the colonies, and pick a single colony with a large colony and a blue halo around it, such as figure 2 As shown, the circled one is No. 11 bacterial strai...

Embodiment 2

[0035] Sequencing and taxonomic identification of strain DB-2:

[0036] 16s rDNA was extracted for PCR amplification: 16s rDNA was amplified by PCR with primer 27F / 1492R; PCR product purification: ExoSApP-IT purification was performed on PCR products with a single band, gel-cutting purification was performed on PCR products with non-specific bands; Sanger Sequencing method, bidirectional sequencing. The spliced ​​sequence of DB-2 bidirectional sequencing, the sequence is shown in SEQ ID No.2, the 16S rDNA sequence and comparison analysis results of strain DB-2 are shown in figure 1 shown. Depend on figure 1It can be seen that DB-2 has two homologous similarities of 100% with Stenotrophomonas sp. in the database, but the two 100% covers are 99, so they are still identified as new strains and preserved and survived.

[0037] Strain DB-2 was morphologically identified through the observation of biological characteristics, and the results are as follows:

[0038] Streak inocul...

Embodiment 3

[0040] Time course of nitrate conversion by DB-2 strain:

[0041] The DB-1 and DB-2 strains were inoculated into the liquid denitrification medium under the same conditions as the reference strain DB-3 with 5% inoculum, and samples were taken every 24 hours to detect the nitrate content of the culture solution. The results are shown in Table 2. It can be seen from Table 2 that in the high-concentration nitrate nitrogen system, both the DB-2 strain and the reference strain DB-3 had an adaptation period. Nitrification performance, the nitrate nitrogen index decreased at a rate of 50% every day, until the fifth day the nitrate nitrogen in the system was completely degraded, and the reference strain showed a slower denitrification process.

[0042] Table 2.Determination results of the total nitrogen curve of DB-2 and DB-3 strains within 6 days

[0043]

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Abstract

The invention relates to stenotrophomonas. The strain is stenotrophomonas (Stenotrophomonas sp.) DB-2 and is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation number is CGMCC No.16355. The DB-2 strain can be used for rapidly degrading nitrate nitrogen under the condition that the bacterium concentration is relatively low after the strain passes througha short adaptive phase; the preparation cost of a microorganism bacterium agent is reduced when the strain is industrially applied and the sewage treatment effect is not influenced; a good denitrification effect can be realized under the condition that the C / N ratio is 4; a few of carbon sources are used and the additional carbon source cost of sewage denitrification treatment is saved; a denitrification process of the DB-2 strain is complete and no intermediate harmful gas including NO and, N2O and the like is not generated, so that the environment pollution is small.

Description

technical field [0001] The invention belongs to the technical field of environmental microbes, and relates to a strain of Stenotrophomonas and its application and microbial agent. Background technique [0002] Nitrogen is one of the indispensable elements of life, which has an important impact on human survival and development. However, in recent years, with the intensification of industrial and agricultural pollution and soil erosion, nitrogen pollution has become increasingly serious. Not only will it cause eutrophication of the water body, but it may also cause harm to humans and animals. A large amount of nitrogen discharge can cause water hypoxia through nitrification, making the water black and smelly; it will stimulate the overgrowth of algae in the water and cause eutrophication; in the nitrogen cycle, except for molecular nitrogen, all nitrogen cycle intermediates The accumulation of such as nitrate and nitrite can have serious impacts on humans and the environment...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/163C12N1/20C12N1/205C12R2001/01
Inventor 包海花杜莉莉许帅
Owner 中原环保股份有限公司
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