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A kind of preparation method of chlorella immunoactive peptide

A technology of immunoactive peptides and chlorella, applied in the field of preparation of chlorella immunoactive peptides, can solve problems restricting the medical value of chlorella

Active Publication Date: 2021-09-14
福建宸润生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no relevant research to prove what kind of components are the main substances that exert high biological activity in Chlorella, and what are the biological activities and functions of different components
Obviously, these research deficiencies have seriously restricted the further development of the medical value of chlorella, especially the development of high-value chlorella health products

Method used

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  • A kind of preparation method of chlorella immunoactive peptide
  • A kind of preparation method of chlorella immunoactive peptide
  • A kind of preparation method of chlorella immunoactive peptide

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Experimental program
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Effect test

Embodiment 1

[0029] 1. Preparation of Chlorella cell wall enzymatic hydrolyzate and specific protein enzymatic hydrolyzate

[0030] 1) The composition ratio of the chlorella cell wall enzymatic hydrolyzate is:

[0031]

[0032] The pH was adjusted to 4.0 with concentrated hydrochloric acid with a mass fraction of 36%.

[0033] 2) The proportioning of the specific proteolytic solution components is:

[0034]

[0035] Adjust the pH to 2.5 with a concentrated hydrochloric acid solution with a mass fraction of 37%;

[0036] 2. Chlorella immunoactive peptides are prepared by the following steps:

[0037] 1) Weigh 500g of chlorella powder and add it to 4.5L of 0.3mol / L NaOH dilute alkali solution, and soak for 60 min at 60°C to obtain the pretreated chlorella liquid;

[0038] 2) The pretreated chlorella liquid obtained in step 1) was homogenized under high pressure for 3 times under 45MPa pressure, centrifuged at 5000rpm for 20min, and the obtained supernatant A was temporarily stored at ...

Embodiment 2

[0067] 1. Preparation of Chlorella cell wall enzymatic hydrolyzate and specific protein enzymatic hydrolyzate

[0068] The cellulase, pectinase and various proteases used were purchased from Beijing Suolaibao Technology Co., Ltd.

[0069] 1) The weight ratio of the chlorella cell wall enzymatic hydrolyzate is:

[0070]

[0071] Adjust the pH to 4.0 with concentrated hydrochloric acid with a mass fraction of 36%;

[0072] 2) The weight ratio of the specific proteolytic solution is:

[0073]

[0074] Adjust the pH to 2.0 with a concentrated hydrochloric acid solution with a mass fraction of 37%;

[0075] 2. Prepared by the following steps:

[0076] 1) Weigh 750g of chlorella powder and add it to 4.25L of 0.3mol / L NaOH dilute alkali solution, and soak for 60min at 60°C to obtain the pretreated chlorella liquid;

[0077] 2) The pretreated chlorella liquid obtained in step 1) was homogenized under high pressure twice under a pressure of 55MPa, centrifuged at 5000rpm for 20m...

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Abstract

The invention provides a preparation method of chlorella immunoactive peptide, which is characterized in that the chlorella protein is extracted by high-pressure homogenization combined with cellulase / pectinase enzymolysis, and after enzymolysis by a specific proteolysis solution, Separation by ultrafiltration membrane to obtain chlorella enzymatic peptides in different molecular weight ranges. Compared with other protease hydrolysis products and polypeptide products of other molecular weights after enzymolysis with the same specific proteolysis solution, the chlorella polypeptide with a molecular weight range of 0-1 kDa after enzymolysis with a specific proteolysis solution has better in vitro Macrophage proliferation and phagocytosis, and the ability to promote delayed-type hypersensitivity in mice and lymphocyte proliferation and transformation in mice. The invention relies on the specific protease hydrolyzate to prepare the chlorella active peptide that better enhances the immunity function, which is beneficial to the development and utilization of health food and medical products that enhance the immunity function.

Description

technical field [0001] The invention provides a preparation method of chlorella immunoactive peptide and belongs to the field of biotechnology. Background technique [0002] Chlorella (Chlorella) is a universal single-celled green algae, which is widely distributed in nature. It can grow and reproduce by using organic carbon sources under heterotrophic conditions. Creatures that grow 4x per hour. Chlorella is extremely nutritious, in which the crude protein content is as high as 50%, the composition of essential amino acids is basically balanced, and it also contains unsaturated fatty acids, biologically active polysaccharides, nucleic acids, vitamins, trace elements, minerals, chlorophyll, etc., with extremely high nutritional value , has various biopharmacological activities such as preventing and treating peptic ulcer, anti-tumor, enhancing immunity, anti-radiation, anti-pathogenic microorganisms, preventing and treating anemia, lowering blood lipid and anti-atherosclero...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06Y02P20/10
Inventor 林陈胜张彦定
Owner 福建宸润生物科技有限公司