Manufacturing method of graphene brilliant cresol blue urinary albumin testing electrode

Abstract

The invention discloses a manufacturing method of a graphene brilliant cresol blue urinary albumin testing electrode. The manufacturing method includes the steps: preparing a PET (polyethylene terephthalate) substrate; manufacturing a graphene working electrode, a reference electrode and a counter electrode; printing the PET substrate in an area except for an insertion end and a reaction area withhigh polymer insulating paint by a printing method to form an insulating layer; uniformly coating a sample reaction area with the graphene working electrode, silver/silver chloride serving as the reference electrode and carbon serving as the counter electrode with crosslinking agent and urinary albumin selective reactant solution by a spin coating method, drying the solution at the temperature of36-38 DEG C, capping a PVC (polyvinyl chloride) insulating film in the reaction area to form an injection port and obtain the graphene brilliant cresol blue urinary albumin testing electrode. The graphene brilliant cresol blue urinary albumin testing electrode has the advantages of short time and high working efficiency in detection. In addition, the content of albumin can be timely detected, anddifficulty in collecting 24-hour urine sample during detection in the prior art is avoided.

Description

Technical field [0001] The invention relates to the field of medical clinical testing, in particular to a method for manufacturing a graphene brilliant cresol blue urine albumin test electrode. Background technique [0002] Urine albumin is an important sensitive indicator in the early stage of kidney disease. It is also the gold standard for non-invasive monitoring of vascular diseases such as kidneys caused by diabetes and hypertension. In recent decades, it has become a major public health problem in China and even in the world. The incidence of chronic kidney disease is increasing year by year worldwide. There are 130 million people with chronic kidney disease in my country, which is followed by cardiovascular and cerebrovascular diseases, diabetes, Tumors are another major disease that directly threatens human health. [0003] However, in the current medical clinical tests, enzyme-linked immunoassay, radioimmunoassay, high-performance liquid chromatography and immunoturbidime...

AI Technical Summary

Problems solved by technology

[0004] The detection time of ELISA is longer; the sensitivity and accuracy of radioimmunoassay are higher, but there is radioactive contamination

[0005] The HPLC process is complex and the operation process is cumbersome

[0006] Immunoscattering turbidimetry is a commonly used method for the determination of urinary albumin in clinical testing. Due to the low content of urinary albumin (normal value less than 20 mg/L), the sensitivity and sp

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