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Traditional Chinese medicine composition with effect of promoting normalization of tumor vessel and preparation method and application thereof

A tumor blood vessel and composition technology, applied in the field of medicine, can solve the problems of increased blood vessel permeability, decreased efficacy of anticancer drugs, hypoxia in tumor microenvironment, etc., and achieves improvement of morphological structure, good clinical application value, and inhibition of liver cancer in situ. The effect of transplanted tumor

Inactive Publication Date: 2019-03-12
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lead to increased vascular permeability, tumor microenvironment hypoxia, acidosis, and greatly reduced efficacy of anticancer drugs

Method used

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  • Traditional Chinese medicine composition with effect of promoting normalization of tumor vessel and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation method of Radix Astragali-Ezhu extract, it comprises the following steps:

[0024] (1) Get 120g Astragalus membranaceus, add 8 times the amount of water, and extract by steam distillation for 1 hour to obtain volatile oil;

[0025] (2) Then take Astragalus medicinal dregs and 120 g of curcuma, add water with a volume 10 times the dry weight of the medicinal materials, soak for 30 minutes, decoct for 30 minutes under a slight boil, and filter to obtain the extracted medicinal liquid. Add 10 times the volume of water to the remaining residue, decoct and extract for 30 minutes in a slightly boiling state; combine the two filtrates, concentrate to 400mL, and then add the zedoary zedoary volatile oil obtained in step (1) to obtain Astragalus membranaceus with a crude drug concentration of 1.2g / mL - The traditional Chinese medicine extract of Zedoary Curcumae, stored in a refrigerator at 4°C for future use.

Embodiment 2

[0027] The Radix Astragali-Ezhu extract prepared in Example 1 was used to affect the angiogenesis structure of zebrafish, as follows:

[0028] 1. Experimental method

[0029] The flk1-EGFP transgenic zebrafish of the TG variety was provided by Nanjing Kaiji Biological Co., Ltd., and cultured in circulating water at a constant temperature of 28°C under a photoperiod of 14h light and 10h dark. The zebrafish mated in pairs naturally, fertilized and spawned the next day, collected the embryos, placed them in a light incubator at 28°C for 6 hours, and selected normal embryos for use. Dissolve the Shenyi capsule with DMSO to make a Shenyi solution with a concentration of 0.16 mg / mL. Dilute the Astragalus-Cezhu traditional Chinese medicine extract prepared in Example 1 with water so that the low, medium and high doses of the Astragalus-Cezhu Chinese medicine extract are 0.6 mg / ml, 0.8 mg / ml and 1.0 mg / ml respectively. Add the above medicinal solution to six-well plates containing s...

Embodiment 3

[0049] The Radix Astragali and Curcuma Radix extracts prepared in Example 1 were used to influence the morphology of blood vessels in mice with liver cancer orthotopic transplantation, as follows:

[0050] 1. Experimental method

[0051] Subcutaneous implantation of liver cancer cells: Culture human liver cancer HepG2 cells to the logarithmic growth phase, digest and make 2.5×10 7 cells / ml of cell suspension. 0.2ml of tumor cell suspension was implanted subcutaneously in nude mice, and visible tumors appeared in about 1 week.

[0052] Establishment of orthotopic xenograft model of human liver cancer in nude mice: the subcutaneous tumor grows to about 1cm in about 3 weeks 3 The tumor-bearing nude mice were sacrificed immediately, the tumor body was removed under aseptic conditions, put into RPMI1640 culture medium, the connective tissue was removed, and the tumor tissue was divided into 1mm 3 tissue fragments. Another batch of nude mice was treated with 1% pentobarbital sod...

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Abstract

The invention discloses a traditional Chinese medicine composition with an effect of promoting normalization of a tumor vessel. The traditional Chinese medicine composition is prepared from traditional Chinese medicines of radix astragali seu hedysari and rhizoma curcumae. An experiment result shows that the traditional Chinese medicine composition provided by the invention is capable of remarkably inhibiting stretching of filopodia of an apical cell and inhibiting zebra fish angiogenesis. The normalization of the tumor vessel is induced in aspects of keeping a blood vessel form uniform and regular, remarkably inhibiting tumor generation and development, remarkably improving a morphological structure of a liver cancer orthotopic transplantation tumor blood vessel, recovering connection ofendothelial cells, increasing integrity of a blood vessel wall, improving maturity of the blood vessel and the like. The composition provided by the invention has the effect of promoting the normalization of the tumor vessel, can be used as an adjuvant drug for clinically treating the tumor, and provides the basis for guiding clinic safe and rational drug use.

Description

technical field [0001] The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition capable of promoting the normalization of tumor blood vessels, and belongs to the technical field of medicine. Background technique [0002] During the occurrence and development of tumors, angiogenesis is almost continuous, and a vascular network is formed through processes such as sprouting, connection, and pruning. Tumor blood vessels not only increase the number of blood vessels, but also have abnormal structure, specifically manifested as tortuous shape, uneven diameter, loose connection of endothelial cells, reduced coverage of pericytes, and variable thickness of basement membrane. Previous treatments have aimed to block angiogenesis, reduce the blood supply to tumor tissue, and limit the supply of oxygen and nutrients to starve tumor cells. This leads to increased vascular permeability, hypoxia in the tumor microenvi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9066A61P35/00
CPCA61K36/9066A61P35/00A61K36/481A61K2236/39A61K2236/331A61K2300/00
Inventor 唐德才尹刚张硕张晓东顾俊菲
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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