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Application of bacteroides fragilis to preparation of enhancing agent for enhancing intestinal mucosal barrier function

A technology of Bacteroides fragilis and enhancer, applied in the application field of Bacteroides fragilis, can solve the problems such as unclear mechanism

Inactive Publication Date: 2019-03-19
GUANGZHOU ZHIYI PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Glutamine plays an important role in maintaining the intestinal epithelial cell barrier function has been accepted by most scholars, but its mechanism is still not very clear

Method used

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  • Application of bacteroides fragilis to preparation of enhancing agent for enhancing intestinal mucosal barrier function
  • Application of bacteroides fragilis to preparation of enhancing agent for enhancing intestinal mucosal barrier function
  • Application of bacteroides fragilis to preparation of enhancing agent for enhancing intestinal mucosal barrier function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Isolation of Bacteroides fragilis and preparation of samples

[0038] (1) Enrichment culture of Bacteroides fragilis and preparation of live bacteria solution

[0039] Streak inoculation of strains on blood plate, anaerobic culture for 48h. Observe the colony morphological characteristics, staining characteristics, size, club shape and distribution, etc.

[0040] Colony characteristics: Bacteroides fragilis ZY-312, after being cultured on a blood plate for 48 hours, is slightly convex, translucent, white, smooth, non-hemolytic, and the diameter of the colony is between 1-3mm. See figure 1 .

[0041] Morphology under the microscope: Bacteroides fragilis ZY-312 was examined by Gram staining. It is a Gram-negative bacterium with a typical rod shape, blunt rounded ends and dense staining. The uncolored part in the middle of the bacteria is like a vacuole. figure 2 .

[0042] Select a single colony and inoculate it in the improved tryptone broth for enrichment...

Embodiment 2

[0043] Example 2 Curative Effect of Bacteroides fragilis on Rat Ulcerative Enteritis

[0044] 1. Experimental method

[0045] In this example, a model of ulcerative colitis (UC) in rats was induced by 2,4-dinitrobenzenesulfonate (DNBS). In this example, 110 Wistar rats, half male and half male, were perfused with 0.5 mL of DNBS (DNBS dissolved in 30% ethanol, ready-to-use) into the intestinal tract of the rats to induce modeling. Each experimental rat is assigned a unique number. Before grouping the rats, the item number, species / strain, sex, cage number and animal number should be marked on the cage label. Rats were randomly grouped according to their initial body weights using BioBook software.

[0046] From Day-2, the rats were fasted for 48 hours. 5% dextrose saline solution (10 mL / kg) was used as an energy supplement for experimental animals during fasting.

[0047] On day 0 of the experiment, fasting rats were anesthetized with Zyta (25mg / kg) and injected 0.5mL, 50m...

Embodiment 3

[0073] Embodiment 3, detection of intestinal mucosal permeability

[0074] The normal level of intestinal mucosal permeability can effectively block the invasion of foreign microorganisms and toxins, which is conducive to the stability of the internal environment of the body and is the guarantee of the health of the body. In this embodiment, the colon segment with the most obvious lesions collected on Day 7 and Day 14 in Example 2 was selected to detect the regulating effect of Bacteroides fragilis provided by the present invention on intestinal permeability, and the following items were detected:

[0075] 1) Histopathological changes: observe tissue changes and inflammatory infiltration by HE staining; observe ultrastructural changes of lesions by transmission electron microscopy, observe epithelial cell morphology, epithelial tight junctions, and changes in epithelial permeability. In this embodiment, the normal control group, the model control group, the positive drug contr...

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Abstract

The invention relates to application of bacteroides fragilis to preparation of an enhancing agent for enhancing an intestinal mucosal barrier function. According to a great quantity of experiments, the bacteroides fragilis has functions of repairing intestinal epithelium mucus barrier and reducing intestinal mucosal permeability and is capable of effectively enhancing the intestinal mucosal barrier function to protect human health.

Description

technical field [0001] The invention relates to the technical field of application of Bacteroides fragilis, in particular to the application of Bacteroides fragilis in preparing an enhancer for enhancing intestinal mucosal barrier function. Background technique [0002] With the deepening of basic medical research, people gradually realized that the intestinal mucosa not only has the function of digestion and absorption, but also has an important defensive barrier function. The intestinal tract is the largest contact surface between the human body and the external environment. There are a large number of bacteria and various toxins in the intestinal cavity, but under normal circumstances, people do not suffer from serious infections or toxic diseases. This is mainly because the intestinal mucosal barrier effectively blocks the invasion of foreign microorganisms and toxins, ensuring the health of the body. However, under various stress conditions or when the intestinal mucos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61P1/00A23C9/12A23G9/36A23L33/135A23L7/104A23K10/18A23C19/00
CPCA23C9/1203A23C19/00A23G9/363A23K10/18A23L7/104A23L33/135A23V2002/00A61K35/741A23V2200/32
Inventor 智发朝王晔刘洋洋白杨王从峰
Owner GUANGZHOU ZHIYI PHARMA INC
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