Pinus koraiensis extract with repairing effect and application thereof
A technology of extract and Korean pine seeds, which is applied in the field of Korean pine extracts, can solve the problems of less application research and no relevant reports of Korean pine nut extracts, and achieves retention of heat-sensitive active ingredients, stability and functional properties. The effect of protecting and increasing the extraction yield
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Embodiment 1
[0047] A kind of Korean pine nut extract, choose Korean pine nut as raw material, extract and obtain through the following steps:
[0048] (1) placing the Korean pine nuts in an oven at 60° C. to dry to a constant weight, and pulverizing through a 40-mesh sieve to obtain the Korean pine nut powder;
[0049] (2) Take the Korean pine nut powder, add a sodium acetate-acetic acid buffer solution with a pH 4.0 that is 3 times the weight of the Korean pine nut, add cellulase, and enzymolyze it in a water bath at 60° C. for 1 hour to obtain an enzymatic hydrolysis solution;
[0050] (3) Add absolute ethanol so that the volume fraction of ethanol in the system is 80%, carry out microwave-assisted leaching in the microwave reactor, the microwave power is 2KW / kg, and the microwave treatment time is 10min to obtain the extract;
[0051] (4) Suction filtration extract, filtrate rotary evaporation removes ethanol, promptly obtains the Korean pine nut extract, and the yield is 58.2%;
[00...
Embodiment 2
[0054] A kind of Korean pine nut extract, choose Korean pine nut as raw material, extract and obtain through the following steps:
[0055] (1) placing the Korean pine nuts in an oven at 60° C. to dry to a constant weight, and pulverizing through an 80-mesh sieve to obtain the Korean pine nut powder;
[0056] (2) Take the Korean pine nut powder, add a sodium acetate-acetic acid buffer solution with a pH 5.5 twice the weight of the Korean pine nut, add cellulase, and enzymolyze it in a water bath at 50° C. for 2 hours to obtain an enzymatic hydrolysis solution;
[0057] (3) Add absolute ethanol so that the volume fraction of ethanol in the system is 60%, carry out microwave-assisted leaching in a microwave reactor, the microwave power is 2.2KW / kg, and the microwave treatment time is 8min to obtain an extract;
[0058] (4) Suction filtration extract, filtrate rotary evaporation removes ethanol, obtains the Korean pine nut extract, and the yield is 60.4%;
[0059] The activity of...
Embodiment 3
[0061] A kind of Korean pine nut extract, choose Korean pine nut as raw material, extract and obtain through the following steps:
[0062] (1) placing the Korean pine nuts in an oven at 60° C. to dry to a constant weight, and pulverizing through a 60-mesh sieve to obtain the Korean pine nut powder;
[0063] (2) Take the Korean pine nut powder, add a sodium acetate-acetic acid buffer solution with a pH 5.0 twice the weight of the Korean pine nut, add cellulase, and enzymolyze it in a water bath at 55° C. for 1 hour to obtain an enzymatic hydrolysis solution;
[0064] (3) Add absolute ethanol so that the volume fraction of ethanol in the system is 70%, carry out microwave-assisted leaching in the microwave reactor, the microwave power is 2.1KW / kg, and the microwave treatment time is 9min to obtain the extract;
[0065](4) Suction filtration extract, filtrate rotary evaporation removes ethanol, obtains the Korean pine nut extract, and the yield is 64.6%;
[0066] The activity of...
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