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A kind of pretreatment method of extracting natural D-borneol from leaves of Plum tree

A natural d-borneol and pretreatment technology, applied in organic chemistry methods, separation/purification of hydroxyl compounds, organic chemistry, etc., can solve problems such as incompleteness, insufficient efficiency, waste of resources, etc., and achieve the goal of improving extraction efficiency Effect

Active Publication Date: 2021-11-19
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction efficiency of prior art is not high enough, the highest extraction efficiency of steam distillation method is about 1.3mg / g, the extraction efficiency of Soxhlet extraction (ethanol extraction) is about 2mg / g
Incomplete extraction, resulting in waste of resources

Method used

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  • A kind of pretreatment method of extracting natural D-borneol from leaves of Plum tree
  • A kind of pretreatment method of extracting natural D-borneol from leaves of Plum tree
  • A kind of pretreatment method of extracting natural D-borneol from leaves of Plum tree

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Synthesis of Deep Eutectic Solvent

[0027] Place the drug used for synthesis (the hydrogen bond acceptor is choline chloride, and the hydrogen bond donor is shown in Table 1) in a drying oven at 45°C for 24 hours, and then accurately weigh the corresponding mass according to the corresponding molar ratio in Table 1. Place the medicine in a flat-bottomed flask, seal it with a parafilm, and transfer it to a water bath at 80°C on a constant temperature magnetic stirrer, heat and stir at a speed of 300rpm for about 2 to 3 hours until a uniform and stable transparent solution is formed, cool to room temperature and place in Store sealed in a desiccator.

[0028] 2. Different depths of eutectic solvent pretreatment of the leaves

[0029] After pulverizing the plum leaves, weigh 1g sample accurately, add 50ml of synthesized deep eutectic solvent respectively, mix well, place on a constant temperature magnetic stirrer at 60°C, heat and stir at 300rpm for 5h. After the tre...

Embodiment 2

[0041] This embodiment is used for the method and condition of pretreatment plum leaf.

[0042] 1. Univariate analysis of pretreatment conditions of plum leaves

[0043]Choline chloride: sucrose molar ratio = 5:2 deep eutectic solvent was selected as the pretreatment solution, and the pretreatment temperature (30°C, 45°C, 60°C, 75°C and 90°C), treatment time (2h, 4h, 6h, 8h and 10h) and deep eutectic solvent water content (choline chloride: sucrose: the molar ratio of water is 5:2:2, 5:2:6, 5:2:10, 5:2: 14 and 5:2:18) on the extraction rate of natural d-borneol. When studying the effect of a certain factor, only this factor is changed, and other factors are kept constant, and the influence of this factor on the extraction efficiency of natural d-borneol is analyzed.

[0044] From Figure 1-3 It can be seen that with the increase of water content, the extraction of plum slice leaves increases first after the pretreatment, and when the water content increases from 6mol to 10m...

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Abstract

The invention belongs to the technical field of biopharmaceuticals, and discloses a pretreatment method for extracting natural d-borneol from leaves of a plum tree. The method is to firstly pretreat the leaves of Prunus chinensis with a deep eutectic solvent, which includes hydrogen bond acceptors, hydrogen bond donors and water; then the pretreated mixture is centrifuged to separate solid particles and used Rinse with clear water, and use Soxhlet extraction method to circulate and extract with absolute ethanol until the color of the extracted liquid does not change. After the extracted liquid is filtered, the content of D-borneol is determined by gas chromatography. The invention adopts the deep eutectic solvent to pretreat the leaves of the plum tree, which can significantly improve the extraction efficiency of extracting natural d-borneol from the leaves of the plum tree. The extraction efficiency of d-borneol can reach 4.936mg / g, which is significantly higher than the extraction efficiency of 2.011mg / g without pretreatment.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and more specifically relates to a pretreatment method for extracting natural d-borneol from the leaves of the plum tree. Background technique [0002] Natural d-borneol is a kind of precious medicinal material and precious spice extracted from lauraceae plant dipterocarp. It has the functions of anti-cancer and cancer treatment, anti-bacterial and anti-inflammatory, swelling and pain relief, expelling wind and refreshing, resuscitating and detoxifying, etc. It is widely used in In the pharmaceutical, food, cosmetics and spice industries, my country mainly relies on imports. In recent years, due to the deforestation of dipterocarp, the origin of Malaysia, the resource plants are almost exhausted, so the price of natural d-borneol is very high. It is imminent to develop and use other resource plants to extract natural d-borneol, so as to alleviate the urgent situation of this raw materia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C29/74C07C35/30
CPCC07B2200/07C07C29/74C07C2602/42C07C35/30
Inventor 傅明辉马笑宇
Owner GUANGDONG UNIV OF TECH