Method for adjusting and controlling production of butyric acid bacteria in intestines through site-oriented release of arabinose

Abstract

The invention relates to a method for adjusting and controlling production of butyric acid bacteria in intestines through site-oriented release of arabinose. The method comprises the following steps of enabling chitosan to dissolve in acetic acid to obtain a chitosan solution, and mixing the chitosan solution with a calcium chloride solution under stirring so as to obtain a chitosan and calcium chloride mixed solution; enabling arabinose to dissolve in a sodium alginate solution to obtain sodium alginate and arabinose mixed liquor, sucking the sodium alginate and arabinose mixed liquor by an injector, dropwise adding the sucked sodium alginate and arabinose mixed liquor into the chitosan and calcium chloride mixed solution, and then collecting generated microspheres; and preparing an araboxylan and sodium alginate mixed solution, then adding laccase, performing uniform mixing, adding the prepared microspheres to obtain mixed liquor, dripping the mixed liquor into the calcium chloride solution by an injector, and collecting obtained arabinose-embedded microcapsule granules. According to the method disclosed by the invention, site-oriented release of the arabinose in the intestines is realized, consumption of the arabinose in gastric juice is reduced, and the physiological function of the arabinose for promoting the growth of butyric acid bacteria and restraining the growth of harmful pathogenic bacteria can be notably improved.

Description

technical field [0001] The invention belongs to the technical field of regulation of butyric acid production in intestines, and in particular relates to a regulation method for fixed-point release of arabinose on butyric acid-producing bacteria in intestines. Background technique [0002] Studies have shown that L-arabinose and sucrose enter the intestinal tract and ferment to produce organic acids, which can significantly improve the intestinal environmental conditions, promote the proliferation of beneficial bacteria such as bifidobacteria, and inhibit the growth of harmful pathogenic bacteria. Therefore, how to prevent arabinose from being consumed in the digestive tract and directly act on the intestinal tract to promote the proliferation of beneficial bacteria such as bifidobacteria and inhibit the growth of harmful pathogenic bacteria is also an urgent problem to be solved. [0003] Butyric acid is the preferred source of colonic energy, 90% of which is dissociated i...

AI Technical Summary

Problems solved by technology

[0005] At present, the main problems of this technology are: first, the embedding effect of microcapsules made of wall materials cannot meet the requirements, and the success rate of embedding is low; second, when the microcapsules pass through the upper gastrointestinal tract, they cannot withstand acidity It is damaged, degraded or swollen by gastric acid, and cannot reach the colon; third, the microcapsules do not have a targeting effect, and the amount of drug reaching the designated site is very small, so the disease cannot be effectively treated and the drug lacks targeting. major issues

However, this method has the following disadvantages: alginate has a microsphere porous structure, so it will form a drug burst release effect, which is not conducive to the absorption of the body, resulting in poor embedding effect, so this invention uses sodium alginate and carrageenan. , but sodium alginate and carrageenan are both anionic polysaccharides, so in theory, no electrostatic interaction of opposite charges will be formed, so that the micropores of sodium alginate gel will be filled, so its embedding success rate and survival rate are lower than others