Preparation and application of carbon quantum dot fluorescent probe

A technology of fluorescent probes and carbon quantum dots, applied in fluorescence/phosphorescence, nano optics, luminescent materials, etc., can solve the problems of low yield of fluorescent carbon quantum dots, poor recognition response sensitivity and anti-interference ability, complex reaction conditions, etc. , to achieve excellent fluorescence properties, good optical performance and stability, simple and safe method

Inactive Publication Date: 2019-03-29
SHANGHAI INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing a carbon quantum dot fluorescent probe and its application, so as to overcome the problems of low yield of fluorescent carbon quantum dots, poor sensitivity and anti-interference ability of recognition response, complex reaction conditions, etc.

Method used

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  • Preparation and application of carbon quantum dot fluorescent probe
  • Preparation and application of carbon quantum dot fluorescent probe
  • Preparation and application of carbon quantum dot fluorescent probe

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Weigh 2 g of anhydrous citric acid into a 50 mL round bottom flask, then add 1 g of ethylenediamine, and heat the mixture at 180° C. for 30 minutes under normal pressure. After cooling, 100 ml of deionized water was added to dissolve the resulting mixture. The above solution was dialyzed with a dialysis bag (MWCO=1000) for 48 hours. After dialysis, the solution was freeze-dried to obtain 0.96 g carbon quantum dot fluorescent probes (N-CDs), which were dissolved in deionized water for further use.

Embodiment 2

[0032] The carbon quantum dot fluorescent probe gained in Example 1 is formulated into an aqueous solution of 0.001g / L, dripped on a copper grid with a carbon film, and tested after vacuum drying, and the particle size distribution of the carbon quantum dot aqueous solution is recorded to be uniform, and The diameter is concentrated at 8-10nm (the particle diameter of more than 40% carbon quantum dot fluorescent probes is at 8-10nm), such as figure 1 shown. And measure its ultraviolet and fluorescence excitation and emission spectra, such as figure 2 As shown, the maximum fluorescence excitation peak and emission peak of the carbon quantum dots are 350nm and 458nm respectively, showing strong blue fluorescence, and the quantum yield is 20.6%.

Embodiment 3

[0034] In this example, the effect of pH on the fluorescence intensity of the carbon quantum dot fluorescent probe was studied. The carbon quantum dot fluorescent probe obtained in Example 1 was formulated into a 0.001 g / L aqueous solution and adjusted with NaOH / HCl aqueous solution to prepare carbon quantum dot solutions with different pHs. Such as image 3 As shown, in solutions with higher or lower pH values, the luminescence intensity decreases, but the luminescence intensity is still greater in weak acid and weak base (4≤pH≤11) solutions. This pH-dependent fluorescence is caused by the protonation and de-excitation of carboxyl or amino groups on the surface of N-CDs, which leads to the electrostatic doping or charging of carbon quantum dot fluorescent probes.

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Abstract

The invention provides a preparation method for a carbon quantum dot fluorescent probe. The preparation method is characterized by comprising the following steps: heating citric acid and quadrol for reaction, dissolving an acquired mixture into water and then putting into a dialysis bag for dialysis, thereby acquiring the carbon quantum dot fluorescent probe. According to the invention, a one-stepmethod is adopted for successfully preparing an environment-friendly, simple and easily acquired carbon quantum dot served as a fluorescent probe; selectivity and fluorescence characteristic of the carbon quantum dot fluorescent probe are combined for recognizing Fe (III); the carbon quantum dot prepared according to the method has the advantages of green, non-toxicity, simple detection method, high selectivity, and the like; a complex modifying process is avoided.

Description

technical field [0001] The invention relates to the preparation and application of a water-soluble fluorescent probe, which belongs to the technical field of preparation of new nanometer materials. Background technique [0002] As a new type of fluorescent nanomaterial, carbon quantum dots have the advantages of adjustable luminous range, easy surface functionalization, good photostability, non-toxicity and good biocompatibility. Once discovered, carbon quantum dots have attracted great research interest. , has shown good application prospects in the fields of cell imaging, biomarkers, analysis and monitoring, drug delivery, photoelectric conversion, and catalysis. Compared with traditional semiconductor quantum dots, fluorescent carbon quantum dots can be synthesized on a large scale in one step, and the synthetic raw materials are cheap and easy to obtain, time-saving and efficient, and can overcome the problems of heavy metal leakage, high toxicity, and serious environmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65G01N21/64B82Y20/00B82Y40/00
CPCB82Y20/00B82Y40/00C09K11/65G01N21/6428G01N21/643G01N2021/6432
Inventor 俞剑玲刘清雷任文博杨振环
Owner SHANGHAI INST OF TECH
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