Fusion protein of avian paramyxovirus, its preparation method, application and apmv vaccine for pigeons

Abstract

The invention provides APMV (avian paramyxovirus) fusion protein, a preparation method and an application thereof and an APMV vaccine for pigeons, and relates to the technical field of biology. The APMV fusion protein is obtained by serial connection of an HN gene of PPMV-1 (pigeon paramyxovirus-1) and an HN gene of APMV-7, and comprises an NDV-HN segment and an AMPV-7-HN segment, wherein the NDV-HN segment comprises the amino acid sequence shown in SEQ ID NO.1, and the AMPV-7-HN segment comprises the amino acid sequence shown in SEQ ID NO.2. The APMV fusion protein has the advantages of goodantigenicity, high expression quantity and wide application.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an avian paramyxovirus fusion protein, its preparation method, application and APMV vaccine for pigeons. Background technique [0002] Recently, there have been many large-scale pigeon farms with tens of thousands to tens of thousands of pairs, and some with 100,000 to 200,000 pairs. Only in Guangdong Province, the annual stock of pigeons has reached more than 4 million pairs, and the production of squabs is more than 45-50 million. It is a large province for raising pigeons in China, and the breeding volume in other provinces and cities is about 3-4 times that of Guangdong. Among them, Jiangsu, Shandong, Hebei, Hunan, Guangxi, and southern Xinjiang and other places are more breeding. Based on such a large-scale breeding environment, it brings huge profits to the pigeon industry. [0003] However, in the process of rapid development of meat pigeon breeding, the occurrence and preval...

AI Technical Summary

Problems solved by technology

Although inactivated vaccines are safe and stable, they require large doses of vaccination or the application of concentrated antigens; the immunity period is short, and booster vaccination is often required; it takes 2 weeks to produce complete immunity, which is not conducive to emergency vaccination and reduced vaccine costs, and the protective effect is not good

However, attenuated virus vaccines are difficult to popularize and apply in practice due to defects such as high cost, easy reversion to ancestors, and potential risk of infection.

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