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Gracilaria chouae polysaccharide and preparation method and application thereof

A technology of Gracilaria brittle and polysaccharides, applied in the field of Gracilaria brittle polysaccharides and its preparation, can solve the problems of poor repeatability, complicated process, poor stability, etc., achieve mild conditions, short time-consuming, simple preparation process, and good stability Effect

Active Publication Date: 2019-04-09
GUANGZHOU JINAN BIOMEDICINE RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on the defects of complex process, poor stability and poor repeatability in the preparation of Gracilaria brittle polysaccharides, and its role in the treatment of inflammation and tumors, it is necessary to provide a product with simple preparation technology, large-scale production, and stable preparation. Gracilaria brittle polysaccharide with good properties and good quality, its preparation method and application

Method used

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  • Gracilaria chouae polysaccharide and preparation method and application thereof
  • Gracilaria chouae polysaccharide and preparation method and application thereof
  • Gracilaria chouae polysaccharide and preparation method and application thereof

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Embodiment 1

[0034] Embodiment 1, Gracilaria brittle polysaccharide of the present invention and preparation method thereof

[0035] The embodiment of the present invention 1 crisp Gracilaria polysaccharide and preparation method thereof, comprises the following steps:

[0036] S1. The fresh crispy Gracilaria is rinsed and drained, dried, crushed and sieved, condensed and refluxed with absolute ethanol for heat treatment, repeated twice, filtered, and the filter residue is dried to obtain the crispy Gracilaria dry powder;

[0037] S2. Soak the dried Gracilaria brittle powder in pure water with a solid-to-liquid ratio of 1:7, extract at 80°C for 3 hours, stir intermittently during the extraction, repeat three times, combine the filtered extracts, and pass through After vacuum concentration by rotary evaporator to obtain concentrated solution, add trichloroacetic acid with a volume 0.3 times the volume of concentrated solution and a mass concentration of 3% to the concentrated solution while...

Embodiment 2

[0041] Embodiment 2, Gracilaria brittle polysaccharide of the present invention and preparation method thereof

[0042] Example 2 of the present invention, Gracilaria brittle polysaccharide and preparation method thereof, comprising the following steps:

[0043] S1. The fresh crispy Gracilaria is rinsed and drained, dried, crushed and sieved, condensed and refluxed with absolute ethanol for heat treatment, repeated twice, filtered, and the filter residue is dried to obtain the crispy Gracilaria dry powder;

[0044] S2. Soak the dried Gracilaria brittle powder in pure water, with a solid-to-liquid ratio of 1:10, extract at 85°C for 3.5 hours, stir intermittently during the extraction, repeat three times, combine the filtered extracts, and extract After vacuum concentration by a rotary evaporator to obtain a concentrated solution, add trichloroacetic acid with a volume of 0.4 times the volume of the concentrated solution and a mass concentration of 5% to the concentrated solutio...

Embodiment 3

[0048] Embodiment 3, the pharmaceutical composition of the present invention for treating esophageal cancer

[0049] The pharmaceutical composition for treating esophageal cancer in Example 3 of the present invention includes Gracilaria brittle polysaccharide and 5-fluorouracil prepared by the preparation method in Example 2 of the present invention.

[0050] The weight ratio of the Gracilaria brittle polysaccharide to 5-fluorouracil is 1:1.

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Abstract

The invention belongs to the technical field of biological medicines and particularly relates to gracilaria chouae polysaccharide and a preparation method and an application thereof. The preparation method comprises the following steps of: pretreating gracilaria chouae through anhydrous ethanol and drying; extracting by using hot water, repeating three times, combining extracts, carrying out vacuum concentration to obtain a concentrated solution, adding a trichloroacetic acid solution into the concentrated solution while stirring, standing at the temperature of 4 DEG C for the night, centrifuging and removing precipitates to obtain supernatant; and adjusting the supernatant to neutrality, carrying out alcohol precipitation till the ethanol concentration is 50-60%, collecting precipitates,adding absolute ethanol, vibrating and washing, standing for centrifugation and carrying out to dialysis and freeze-drying to obtain the gracilaria chouae polysaccharide. The invention also provides an application of the gracilaria chouae polysaccharide to synergist of anti-inflammatory or anti-tumor chemotherapeutic drugs. The preparation method of the gracilaria chouae polysaccharide is simple and stable; and the prepared gracilaria chouae polysaccharide is obvious in the synergistic effect of the anti-inflammatory or anti-tumor chemotherapeutic drugs and is suitable for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a Gracilaria brittle polysaccharide and its preparation method and application. Background technique [0002] Gracilaria brittle is a large economical cultured seaweed of the genus Gracilaria. It is mainly produced in the southeastern coastal areas of Zhejiang, Fujian, and Jiangsu. It is currently mainly distributed in the coastal areas of Nan'ao, Guangdong Province. It has been successfully cultured and can be planted on a large scale. Fresh crispy Gracilaria is purple-red or light red in color, plump and juicy, crisp in quality, and becomes darker after drying. Due to the scarcity of the yield of Gracilaria brittle in the early stage, it has not been paid attention to, so there are few researches on Gracilaria brittle at home and abroad. Gradually attract people's attention. Studies have shown that Gracilaria brittle is rich in a variety of active polysacchari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61K31/513A61P35/00A61P29/00A23L33/125
CPCA23V2002/00A61K31/513A61K31/715A23L33/125A61P29/00A61P35/00C08B37/00C08B37/0003A23V2200/308A23V2200/30A23V2250/202A23V2250/51A61K2300/00
Inventor 赵振岭王一飞
Owner GUANGZHOU JINAN BIOMEDICINE RES & DEV CENT