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Fermentation medium and method for high-density pediococcus pentosaceus

A technology of Pediococcus pentosaceus and fermentation medium, which is applied in the field of fermentation medium of high-density Pediococcus pentosaceus, can solve the problems of production gap and low concentration of Pediococcus pentosaceus, achieves the promotion of bacterial growth and is beneficial to Growth, the effect of reducing production costs

Inactive Publication Date: 2019-04-09
湖北华扬科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the Shake Flask method of Pediococcus Pentosacea in general reports, the concentration of Pediococcus Pentosacea in the fermentation broth is not high, and most of the reports only stay at the level of shake flasks in the laboratory, and there is still a big gap from the realization of production

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  • Fermentation medium and method for high-density pediococcus pentosaceus
  • Fermentation medium and method for high-density pediococcus pentosaceus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The fermentation method of high-density Pediococcus pentosaceae provided by the present embodiment comprises the steps:

[0041] S1. A single colony of Pediococcus pentosaceus was isolated and preserved

[0042] Firstly, insert the Pediococcus pentosaceae strain into the isolation medium, and sterilize it at 115°C for 30 minutes, then import it into a plate culture dish to cool and solidify, and use an inoculation loop to dip the bacterial solution on the plate under sterile conditions. Streak the plate on the culture medium, then invert the plate, and culture it in a 37°C incubator for 24 hours to obtain a single colony of Pediococcus pentosaceus.

[0043] Among them, the components of the separation medium include: yeast extract powder 2%, tryptone 1.5%, beef powder 1%, glucose 1.5%, magnesium sulfate 0.05%, sodium acetate trihydrate 0.5%, manganese sulfate 0.01%, phosphoric acid trihydrate Dipotassium hydrogen 0.2%, Tween-800.8% and agar powder 0.5%, the balance is ...

Embodiment 2

[0056] The fermentation method of high-density Pediococcus pentosaceae provided in this example is basically the same as Example 1, the difference is that the content of yeast autodissolving powder in the fermentation medium in this example is 1%.

[0057] After 27 hours of anaerobic fermentation, the fermentation liquid was taken, and the number of Pediococcus pentosaceae in the fermentation liquid was determined to reach 9.5 billion CFU / mL by plate colony counting method.

Embodiment 3

[0059] The fermentation method of high-density Pediococcus pentosaceae provided in this example is basically the same as that in Example 1, except that the content of yeast autodissolving powder in the fermentation medium in this example is 1.5%.

[0060] After 27 hours of anaerobic fermentation, the fermentation liquid was taken, and the number of Pediococcus pentosaceae in the fermentation liquid was determined to reach 23.2 billion CFU / mL by plate colony counting method.

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Abstract

The invention relates to the technical field of microbial fermentation, in particular to a fermentation medium and method for high-density pediococcus pentosaceus. By the disclosed fermentation medium, yeast autolytic powder is added as a nitrogen source, sodium hydroxide cooperates with calcium carbonate, pH is easy to regulate and control, good conditions are provided for growth and yield of thepediococcus pentosaceus, and therefore, the high-density pediococcus pentosaceus is obtained. The invention further discloses the fermentation method for the high-density pediococcus pentosaceus. Thefermentation method for the high-density pediococcus pentosaceus comprises the following steps: inoculating a pediococcus pentosaceus strain in a separation medium to obtain pediococcus pentosaceus single colonies, inoculating the pediococcus pentosaceus single colonies into a seed medium for culturing, and storing the pediococcus pentosaceus single colonies; during use, directly inoculating thestored pediococcus pentosaceus single colonies into an activating medium and carrying out activation and enlarge culturing; and inoculating a culture solution of the activating medium into the fermentation medium and carrying out anaerobic fermentation to obtain high-density pediococcus pentosaceus fermentation liquor.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a fermentation medium and a fermentation method of high-density Pediococcus pentosaceae. Background technique [0002] Pediococcus pentosaceus (Pediococcus pentosaceus) belongs to the genus Pediococcus of Streptococcus family, is a Gram-positive bacterium, can produce lactic acid by fermenting glucose, and belongs to a kind of lactic acid bacteria. As a probiotic, Pediococcus pentosacea has been used in food preservation, dairy product fermentation, animal microecological preparations and other fields. Pediococcus pentosaceus, as an inherent species of intestinal microorganisms in animals, can effectively inhibit the proliferation of various pathogenic bacteria and maintain the health of animals through its own reproduction. Disease resistance, enhanced systemic and local immune function. All of these make the probiotic function of Pediococcus pentosacea get more...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 吴祥张茜茜周樱谌颉
Owner 湖北华扬科技发展有限公司