Method for improving electroporation efficiency of electric energy induced living cell membrane

A technology of electroporation and cell membrane, applied to other methods of inserting foreign genetic materials, electric/wave energy processing enzymes, biochemical equipment and methods, etc., can solve the problems of irreversible electroporation of cell membranes, etc.
CN109593753AInactive Publication Date: 2019-04-09ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2019-04-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for improving electroporation efficiency of an electric energy induced living cell membrane. Electric energy stored in a capacitor is released into an iso-osmia normalsaline solution in a mode of index waveform nanosecond electric pulse through a conduction wire, and then cell membranes of living cells in the normal saline solution have electroporation. When the impedance of the normal saline solution is greater than the characteristic impedance of the conduction wire, as charges are reflected in the conduction wire, the voltage released into the loaded electric pulse is higher than that loaded at a capacitor discharge start point, then when same single pulse energy stored in the capacitor is released into living cells suspended in the normal saline solution, the voltage of the pulse has a higher highest peak value, and the efficiency that induced living cells have functional electroporation is improved. Living cells of a greater ratio can be induced to have cell membrane electroporation by outputting less total energy.
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Description

technical field

[0001] The invention relates to a method for improving the electroporation efficiency of living cell membranes induced by electric energy. technical background

[0002] Living cells in biological tissues are isolated from the microenvironment by a cell membrane composed of a layer of phospholipid bimolecules, which is the basis for cells to survive and proliferate in the environment. Previous studies have believed that when a square wave or trapezoidal electrical pulse is released into biological tissue, when the pulse width of the pulsed electric field exceeds 30 microseconds (us), the field strength exceeds 800 volts / centimeter (V / cm), and more than 8 electrical pulses Inducing cell membrane cell membrane perforation, this is the current application of high-voltage pulsed electric fields in the biomedical field, and the use of high-intensity pulsed electric fields can cause reversible or irreversible electroporation of cell membranes. Reversible cell membr...

Claims

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