Application of SNase in preparation of medicament for treating inflammatory bowel disease

A technology for inflammatory bowel disease and drugs, which is applied in the fields of pharmacy and medicine, can solve the problems of high recurrence rate, adverse reactions, poor patient compliance, etc. The effect of production costs

Active Publication Date: 2019-04-12
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the traditional drugs marketed for the treatment of IBD, such as aminosalicylic acids mainly include sulfasalazine, etc., but they cannot eradicate the disease, and there is a high recurrence rate, while glucocorticoids have many adverse reactions, Improper use can easily cause shock. At present, common biological agents include anti-TNF monoclonal antibodies such as infliximab, adalimumab, and cytokine inhibitors, but biolo

Method used

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  • Application of SNase in preparation of medicament for treating inflammatory bowel disease
  • Application of SNase in preparation of medicament for treating inflammatory bowel disease
  • Application of SNase in preparation of medicament for treating inflammatory bowel disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the preparation of SNase oral preparation

[0023] Mix 57ml of span 85 and 22.8ml of absolute ethanol into a round-bottomed flask, stir magnetically in a water bath at 40°C for 10min, then add 8ml of 0.25% sodium alginate solution (drug loading 5mg SNase) dropwise, continue stirring for 10min, then Pump out the water until it becomes clear, pour 1ml of calcium chloride ethanol solution (0.1%) into it, and continue pumping and stirring for 30 minutes to solidify the nanoparticles until there are no bubbles. Centrifuge the mixed solution at 8000rpm for 30min, collect the precipitate, wash twice with petroleum ether, collect the precipitate, resuspend the precipitate with distilled water, and ultrasonically disperse with an ultrasonic cell pulverizer, 400W, 50 times / min to obtain SNase nanoparticle suspension liquid.

Embodiment 2

[0024] Example 2: Pharmacodynamic evaluation of SNase oral preparation against inflammatory bowel disease.

[0025] Twenty-one 8-week-old female C57BL / 6J mice (purchased from Qinglongshan Animal Breeding Factory, Jiangning District, Nanjing, license number: SCXK (Su) 2017-0001) were randomly divided into 3 groups, which were the blank group and the model group. group and the calcium alginate immobilized SNase group (i.e., the nanoparticle suspension prepared in Example 1), 7 mice in each group, and adaptively fed for three days. The solvent is made into 3% DSS (dextran sulfate sodium), so that the mice drink water freely, and embodiment 1 obtains the SNase nanoparticle suspension SNase (2.5mg / ml) group begins intragastric administration on the day of modeling, each The mice were given 200ul orally, and the model was established for 7 days, once a day, and the mice were killed by cervical dislocation on the eighth day, and the pharmacodynamics score was performed.

[0026] (1)...

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Abstract

The invention relates to the application of staphylococcal nuclease (SNase) in preparation of a medicament for preventing or treating inflammatory bowel disease. The object of the present invention isto provide a novel use of SNase in action of resisting inflammatory bowel disease. Results show that SNase can significantly alleviate colonic inflammation in C57BL/6J mice, can better control the body weight, colon length, fecal occult blood and inflammatory infiltration of the C57BL/6J mice, and significantly improve the survival rate of the C57BL/6J mice. Therefore, the SNase can significantlyalleviate intestinal inflammation in the C57BL/6J mice, and can be used for development of medicaments for prevention and treatment of inflammatory bowel diseases.

Description

technical field [0001] The invention relates to a nuclease SNase with anti-inflammatory bowel disease function, as well as derivatives containing the nuclease and similar pharmaceutical preparations. The invention relates to the related fields of pharmacy and medicine. Background technique [0002] Inflammatory bowel disease (IBD) is a group of chronic intestinal diseases of unknown etiology, including inflammatory bowel disease (Ulcerative Colitis, UC) and Crohn's disease (Crohn's disease, CD). It is common in western countries, and the prevalence in European and North American countries is as high as 120-200 / 100,000. It is a common disease in the digestive field. With the change of lifestyle and the improvement of living standards, the incidence of IBD in our country presents an increasing trend year by year, and has become a common disease of the digestive system and the main cause of chronic diarrhea and bloody stool in our country. There are differences in the location...

Claims

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Application Information

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IPC IPC(8): C07K14/31A61K38/46A61P1/00
Inventor 吴洁董万法张婷婷
Owner CHINA PHARM UNIV
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