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Preparing method of injectable sodium hyaluronate gel containing microballoons

A technology of sodium hyaluronate and microspheres, which is applied in the fields of medicine and cosmetic filling materials, can solve the problems of polylactic acid microsphere deposition and other problems, and achieve the effect of simple method, feasible operation and no irritating effect.

Inactive Publication Date: 2019-04-16
长春杭盖生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, simply mixing polylactic acid microspheres with the salt solution of cross-linked hyaluronic acid gel by physical blending can prolong the retention time of the product in body tissues, but it still cannot solve the problem of hyaluronic acid being absorbed. , technical issues of polylactic acid microsphere deposition

Method used

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  • Preparing method of injectable sodium hyaluronate gel containing microballoons
  • Preparing method of injectable sodium hyaluronate gel containing microballoons
  • Preparing method of injectable sodium hyaluronate gel containing microballoons

Examples

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Effect test

Embodiment 1

[0037] A preparation method of an injectable gel containing polylactic acid microspheres, specifically comprising the following steps:

[0038] Step S1, dissolving 2 g of sodium hyaluronate with a molecular weight of 200,000 to 400,000 Daltons in 98 mL of pure water to obtain an aqueous solution A with a solution concentration of 2%;

[0039] Step S2, adding 5 g of PLLA microspheres (L-polylactic acid microspheres, particle size of 20-40 nm, molecular weight of 100,000 Daltons) material to solution A, and uniformly dispersed suspension B was obtained after uniform stirring;

[0040] Step S3, adjusting the pH value of the suspension B to between 4.75-5.25 (1mol / L hydrochloric acid), to obtain the suspension C;

[0041] Step S4, add cross-linking agent and catalyst to the suspension C, the cross-linking agent is carbodiimide and adipic acid dihydrazide, and the catalyst is 1-hydroxybenzotriazole (sodium hyaluronate: dicarbide Imine: adipic acid dihydrazide: 1-hydroxybenzotriazole...

Embodiment 2

[0048] A preparation method of an injectable gel containing polycaprolactone microspheres, specifically comprising the following steps:

[0049] Step S1, dissolving 2 g of sodium hyaluronate with a molecular weight of 200,000 to 400,000 Daltons in 98 mL of pure water to obtain an aqueous solution A with a solution concentration of 2%;

[0050] Step S2, adding 5g of PCL microspheres (polycaprolactone, particle size 20-40nm, molecular weight at 50,000 Daltons) material to solution A, and evenly stirring to obtain a uniformly dispersed suspension B;

[0051] Step S3, adjusting the pH value of the suspension B to between 4.75-5.25 (1mol / L hydrochloric acid), to obtain the suspension C;

[0052] Step S4, add cross-linking agent and catalyst to the suspension C, the cross-linking agent is carbodiimide and adipic acid dihydrazide, and the catalyst is 1-hydroxybenzotriazole (sodium hyaluronate: dicarbide Imine: adipic acid dihydrazide: 1-hydroxybenzotriazole (1:3:1:3), and stirred un...

Embodiment 3

[0059] A preparation method for an injectable gel containing PLCL microspheres, specifically comprising the following steps:

[0060] Step S1, dissolving 2 g of sodium hyaluronate with a molecular weight of 1 million to 3 million Daltons in 48 mL of pure water to obtain an aqueous solution A with a solution concentration of 4%;

[0061] Step S2, add 2.5g PLCL microspheres (poly-L-lactic acid-polycaprolactone, particle size is 20-40nm, molecular weight is 30,000 Daltons) material to solution A, obtain uniformly dispersed suspension after uniform stirring Liquid B;

[0062] Step S3, adjusting the pH value of the suspension B to between 4.75-5.25 (1mol / L hydrochloric acid), to obtain the suspension C;

[0063] Step S4, add cross-linking agent and catalyst to the suspension C, the cross-linking agent is carbodiimide and adipic acid dihydrazide, and the catalyst is 1-hydroxybenzotriazole (sodium hyaluronate: dicarbide Imine: adipic acid dihydrazide: 1-hydroxybenzotriazole (1:3:1:...

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Abstract

The invention discloses a preparing method of injectable sodium hyaluronate gel containing microballoons. The preparing method comprises the following steps of dissolving sodium hyaluronate into purewater to obtain a solution A; adding a microballoon material into the solution A, and conducting uniform stirring to obtain a homodisperse suspension liquid B; adjusting the PH value of the suspensionliquid B to obtain a suspension liquid C; adding a crosslinking agent into the suspension liquid C, conducting uniform stirring to obtain a homodisperse suspension liquid D, after a reaction is carried out for a certain period of time, crosslinking sodium hyaluronate in the suspension liquid D to form colloid E containing the homodisperse microballoon material; smashing the colloid E to obtain gel particles F, and obtaining gel particles G through screens with different mesh numbers; removing soluble impurities and crosslinking agents in the gel particles G in a repeated soaking or dialytic mode to obtain gel particles H; utilizing a PBS buffering solution for balancing for repeatedly soaking the gel particles H to obtain gel particles I; obtaining the sodium hyaluronate gel through the procedures of semi-dehydration, filling and sterilization. By means of the preparing method, the problem that the microballoon material is deposited and gets caked is effectively solved.

Description

technical field [0001] The invention belongs to the field of medicine, and also belongs to the field of cosmetic filling materials, and specifically relates to a preparation method of injectable sodium hyaluronate gel containing microspheres. Background technique [0002] Hyaluronic acid, also known as hyaluronic acid and hyaluronic acid, is a natural polysaccharide structure composed of repeated D-glucuronic acid and N-acetylglucose through β-1,3-glucosidic bonds. It exists in the interstitium of animal cells and is an important basic substance for skin suppleness. It has strong water absorption and special water retention. It is the most moisturizing substance found in nature so far. It is called an ideal natural moisturizing factor. In addition, hyaluronic acid can also improve skin nutrient metabolism, make skin soft, smooth, wrinkle-free, increase elasticity, and prevent aging. It is also a good transdermal absorption accelerator while moisturizing. To the more ideal e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/18A61L27/16A61L27/52A61L27/58C08J3/075C08J3/24C08L67/04C08L5/08C08K5/29C08K5/25
CPCA61L27/16A61L27/18A61L27/20A61L27/52A61L27/58A61L2400/06A61L2430/34C08J3/075C08J3/246C08J2367/04C08J2405/08C08K5/25C08K5/29C08L5/08
Inventor 王媛邵云鹏
Owner 长春杭盖生物科技有限公司
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