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Application of virtual template in preparation of tentoxin molecularly imprinted polymer and preparation method of tentoxin molecularly imprinted polymer

A technology of molecular imprinting and virtual template, applied in chemical instruments and methods, other chemical processes, etc., can solve problems such as difficult separation of tentoxin, shorten the preparation cycle, improve detection efficiency and accuracy, and achieve the effect of high imprinting factor

Active Publication Date: 2019-04-16
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the problem that there is no molecularly imprinted polymer of toxin at present, and there is no virtual template that can replace the template molecule of toxin and be used to prepare the molecularly imprinted polymer of toxin, which leads to the difficulty of separating tentoxin. Application of a Virtual Template in Preparation of Molecularly Imprinted Polymer of Tentoxin and Preparation Method of Molecularly Imprinted Polymer of Tentoxin

Method used

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  • Application of virtual template in preparation of tentoxin molecularly imprinted polymer and preparation method of tentoxin molecularly imprinted polymer
  • Application of virtual template in preparation of tentoxin molecularly imprinted polymer and preparation method of tentoxin molecularly imprinted polymer

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Embodiment 1

[0035] A preparation method of tenimotoxin molecularly imprinted polymer, comprising the following steps:

[0036] (1) Add 164mg of dummy template into a 150ml round-bottomed flask containing 50ml of acetonitrile and DMSO mixed solvent, wherein the volume ratio of acetonitrile and DMSO is 6:1, add 171mg of methacrylamide, stir at room temperature for 1h, add 1.98g Dimethacrylate oxalate, 72mg of azobisisobutyronitrile, then degassed by ultrasonic for 10min, placed on ice-water bath at 0-4℃ to deoxygenate by bubbling nitrogen for 10min, sealed and stirred for 24h, the reaction temperature was 60 ℃ to obtain molecularly imprinted polymers with virtual template molecules;

[0037] (2) Centrifuge the molecularly imprinted polymer with virtual template molecules obtained in step (1) at a speed of 10,000 rpm for 15 minutes to collect polymer particles;

[0038] (3) Add the polymer particles into a mixed solvent of methanol and acetic acid, the volume ratio of methanol and acetic ac...

Embodiment 2

[0040] A preparation method of tenimotoxin molecularly imprinted polymer, comprising the following steps:

[0041] (1) Add 202mg of dummy template into a 150ml round bottom flask containing 50ml of acetonitrile and DMSO mixed solvent, wherein the volume ratio of acetonitrile and DMSO is 6:1, add 606mg of methacrylamide, stir at room temperature for 1h, add 3.02g Dimethacrylate oxalate, 100mg azobisisobutyronitrile, then ultrasonically degassed for 10min, placed on an ice-water bath at 0-4°C for 10min with nitrogen bubbling to remove oxygen, sealed and stirred for 24h, the reaction temperature was 60 ℃ to obtain molecularly imprinted polymers with virtual template molecules;

[0042] (2) Centrifuge the molecularly imprinted polymer with virtual template molecules obtained in step (1) at a speed of 10,000 rpm for 15 minutes to collect polymer particles;

[0043](3) Add the polymer particles into a mixed solvent of methanol and acetic acid, the volume ratio of methanol and aceti...

Embodiment 3

[0045] A preparation method of tenimotoxin molecularly imprinted polymer, comprising the following steps:

[0046] (1) Add 250 mg of dummy template into a 150 ml round bottom flask containing 55 ml of acetonitrile and DMSO mixed solvent, where the volume ratio of acetonitrile and DMSO is 6:1, add 1.75 g of methacrylamide, stir at room temperature for 1 hour, add 4.45 g dimethacrylate oxalic acid ester, 80mg azobisisobutyronitrile, then degassed by ultrasonic for 10min, placed on an ice-water bath at 0-4°C for 10min by bubbling nitrogen to remove oxygen, sealed and stirred for 24h, the reaction temperature is At 60°C, molecularly imprinted polymers with virtual template molecules were obtained;

[0047] (2) Centrifuge the molecularly imprinted polymer with virtual template molecules obtained in step (1) at a speed of 10,000 rpm for 15 minutes to collect polymer particles;

[0048] (3) Add the polymer particles into a mixed solvent of methanol and acetic acid, the volume ratio ...

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Abstract

The invention discloses application of a virtual template in preparation of a tentoxin molecularly imprinted polymer and a preparation method of the tentoxin molecularly imprinted polymer. The virtualtemplate is a tentoxin molecular structure analogue searched in a free commercial database, is available in the market, can replace tentoxin template molecules to prepare the tentoxin molecularly imprinted polymer and fills the market vacancy of the tentoxin molecularly imprinted polymer. In addition, the tentoxin molecularly imprinted polymer has high imprinting factor content and shows an excellent tentoxin binding characteristic, and a new idea is provided for solving the problem of difficult separation and enrichment in existing tentoxin detection and pre-treatment process. In addition, design and synthesis of alternative templates are not needed, a preparation cycle of the tentoxin molecularly imprinted polymer is greatly shortened, the consumption of labor and experimental drugs isreduced, and the tentoxin molecularly imprinted polymer has the guiding role in improvement of the research and development efficiency of molecularly imprinted polymers and widening of the applicationrange of molecular imprinting techniques.

Description

technical field [0001] The invention belongs to the technical field of preparing molecularly imprinted polymers, and in particular relates to the application of a virtual template in the preparation of tentoxin molecularly imprinted polymers and a preparation method of tenentoxin molecularly imprinted polymers. Background technique [0002] At present, the detection methods of mycotoxins have developed rapidly, and have developed from traditional thin-layer chromatography (TLC) to modern chromatography, chromatography-mass spectrometry, and immuno-mass spectrometry techniques, such as gas chromatography-mass spectrometry (GC-MS), liquid Mass chromatography (HPLC-MS), and immunoassay methods based on immunochemistry, such as enzyme-linked immunosorbent assay (ELISA), etc. By comparing the above detection methods, we found that the chromatographic detection method is time-consuming and requires expensive immunoaffinity columns for sample pretreatment, which is difficult to use...

Claims

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Application Information

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IPC IPC(8): C08F222/14C08F220/56C08J9/26B01J20/26B01J20/30
CPCB01J20/268C08F222/1006C08F222/102C08J9/26C08F220/56
Inventor 张薇薇邓晓东张弛阮佳伏俊桦李陈杰周莉郭紫薇彭宏浩
Owner CHENGDU MEDICAL COLLEGE
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