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Trichoderma viride fermentation method and water dispersible granule thereof

A technology of Trichoderma viride and fermentation methods, applied in the direction of using spores, methods based on microorganisms, biochemical equipment and methods, etc., to achieve the effects of improving stress resistance and extending shelf life

Active Publication Date: 2019-09-27
云南星耀生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Compared with conidia, chlamydospores have the advantages of strong stress resistance and long shelf life, but in the prior art, Trichoderma viride LTR-2 can only be obtained by fermenting liquid rich in conidia

Method used

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  • Trichoderma viride fermentation method and water dispersible granule thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation of embodiment 1 Trichoderma viride LTR-2 chlamydospore fermented liquid

[0024] This example describes the preparation of Trichoderma viride LTR-2 chlamydospore fermentation broth.

[0025] 1. Activation of Trichoderma viride LTR-2

[0026] From the slant of Trichoderma viride LTR-2, use a sterile inoculation needle to pick part of the lawn into the prefabricated eggplant bottle containing PDA solid medium, culture at 30°C for 2 days, and obtain activated Trichoderma viride LTR-2 .

[0027] 2. Preparation of Trichoderma viride LTR-2 seed solution

[0028] Pick up the activated Trichoderma viride LTR-2 lawn with a sterile inoculation needle, and inoculate it into a Erlenmeyer flask (500 mL size) containing 100 mL of liquid seed medium. Culture conditions: In a shaker at 28°C and 100-150 r / min, shake and culture in the shade for 2-3 days to obtain Trichoderma viride LTR-2 seed liquid.

[0029] The liquid seed medium is prepared by the following method...

Embodiment 2

[0034] The preparation of embodiment 2 Trichoderma viride LTR-2 water dispersible granules

[0035] The chlamydospore content was 15 x 10 8 In the cfu / mL Trichoderma viride LTR-2 fermentation liquid (prepared in Example 1), after adding filter aid, filter or add flocculant and then centrifuge to obtain the chlamydospore concentrate. For example: the content of chlamydospores to 1200kg is 15×10 8 Add flocculant polyacrylamide (according to the ratio of 2g / kg) to the cfu / mL Trichoderma viride LTR-2 fermented liquid, mix evenly, centrifuge and concentrate at 5000rpm in a butterfly centrifuge, take the precipitate, and get 210kg of chlamydospore concentrate.

[0036] In 210kg of chlamydospore concentrate, add 1000 mesh kaolin (filler) 290kg, then add sodium lauryl sulfate (wetting agent) 10kg, sodium lignosulfonate (dispersant) 25kg and sodium carboxymethyl starch ( Disintegrant) 15kg, mix in the mixing tank for more than 60min, and stir evenly. The obtained mixture is pulveri...

Embodiment 3

[0043] Example 3 Control effect of Trichoderma viride LTR-2 water dispersible granule on disease in the field

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Abstract

The invention provides a trichoderma viride fermentation method and a water dispersible granule thereof, and relates to the field of microbial pesticides. The trichoderma viride fermentation method comprises the following steps: inoculating trichoderma viride LTR-2 to a fermentation culture medium, controlling the temperature at 29-31 DEG C at the early stage of the fermentation, and controlling the temperature at 24-26 DEG C and the pH value at 5.0-5.5 at the late stage of the fermentation; obtaining a trichoderma viride LTR-2 fermentation liquor rich in chlamydospores. By adopting the method, the trichoderma viride fermentation liquor rich in chlamydospores can be obtained, the stress resistance of the product is obviously improved, and the shelf life is prolonged; The effect of the water dispersible granule on controlling tomato gray mold, potato late blight and tobacco powdery mildew is obviously superior to that of a control medicament and agricultural preparation DZ-1.

Description

technical field [0001] The invention relates to the field of microbial pesticides, in particular to a fermentation method of Trichoderma viride and its water-dispersible granules. Background technique [0002] Trichoderma belongs to the subphylum Deuteromycetes, Hyphospora, Hyphospora, Myxospora, and is a class of ubiquitous fungi. [0003] Trichoderma viride ( Trichoderma viride ) is widely distributed in nature, often saprophytic on wood, seeds and plant residues. Trichoderma viride can produce a variety of enzymes with biological activity, such as: cellulase, chitinase, xylanase and so on. It plays an important role in the biological control of plant pathology. Trichoderma viride is one of the strains with the highest cellulase activity, and the cellulase produced can degrade crops very well. [0004] Most Trichoderma viride can produce a variety of biologically active substances that have antagonistic effects on phytopathogenic fungi, bacteria and insects, and can im...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N3/00C12N1/04C12R1/885
Inventor 仇俊涛杨合同罗树荣
Owner 云南星耀生物制品有限公司