Complex probiotic and application thereof

A technology of compound probiotics and bifidobacteria, which is applied in the field of microorganisms, can solve the problems of few researches on the compound formula of probiotics

Active Publication Date: 2019-04-16
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above patents all use a single probiotic or a combination of probiotics and prebiotics or Chinese herbal medicines to relieve metabolic syndrome, and currently there are few probiotic compound formulas that can synergistically improve metabolic syndrome. Research

Method used

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  • Complex probiotic and application thereof
  • Complex probiotic and application thereof
  • Complex probiotic and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0043] The present invention also provides a preparation method for the composite probiotics, in which Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631, Lactobacillus rhamnosus CCFM1044 and Lactobacillus casei CCFM711 are respectively inoculated into the improved MRS medium, Cultivate under anaerobic conditions at 34-39°C for 18-24 hours, collect the bacteria; resuspend with freeze-drying protectant to make the content of each bacteria reach 10 10 CFU / mL or more, then the suspension was cultured under anaerobic conditions at 37°C for 40-60 minutes, dried to obtain freeze-dried bacterial powder of each bacterial cell, and the number of viable bacteria in the bacterial powder was confirmed by resuspension and dilution plate coating; The Bifidobacterium adolescentis CCFM8630 freeze-dried bacteria powder, Lactobacillus reuteri CCFM8631 freeze-dried bacteria powder, Lactobacillus rhamnosus CCFM1044 and Lactobacillus casei CCFM711 freeze-dried bacteria powder wer...

Embodiment 1

[0076] Example 1: Compound probiotics have good tolerance to simulated gastrointestinal fluid

[0077] Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631, Lactobacillus rhamnosus CCFM1044 and Lactobacillus casei CCFM711 stored at -80°C were inoculated in the modified MRS medium (mMRS) respectively. Cultivate at 37°C for 36 hours, then subculture twice with an inoculation amount of 2%-4%, adjust the concentration of viable bacteria in the culture solution of Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631, Lactobacillus rhamnosus CCFM1044 and Lactobacillus casei CCFM711 5×10 8 CFU / mL, take the concentration as 5×10 8 CFU / mL of Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631, Lactobacillus rhamnosus CCFM1044 and Lactobacillus casei CCFM711 culture solution 1mL each, mix well, take 1mL of mixed culture solution and 9.0mL pH 2.5 artificial gastric juice (mMRS medium containing 1% pepsin, pH=2.5) mixed, cultured in an...

Embodiment 2

[0084] Example 2: Compound probiotics have no toxic side effects on C57BL / 6J mice

[0085] Suspend the compound probiotic bacteria with the ratio of Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631, Lactobacillus rhamnosus CCFM1044 and Lactobacillus casei CCFM711 with a ratio of 1:1:1:1 viable bacteria in 3% sucrose solution , made at a concentration of 1.0×10 9 CFU / mL bacterial suspension. Eight healthy male C57BL / 6J mice with a body weight of about 20g-22g were taken, and after one week of adaptive feeding, they were fed with the bacterial suspension of this concentration once a day, observed for one week, and the death and body weight were recorded. The experimental results are shown in Table 3.

[0086] Table 3 Changes in body weight and death of mice

[0087] time (days)

1

2

3

4

5

6

7

weight(g)

21.1±0.9

21.3±1.1

21.5±1.3

21.6±1.2

21.9±0.8

22.2±0.8

22.5±0.9

Circumstances of death

-...

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Abstract

The invention relates to the technical field of microorganisms, and discloses complex probiotic and use thereof. The complex probiotic consists of bifidobacterium adolescentis CCFM8630, lactobacillusreuteri CCFM8631, lactobacillus rhamnosus CCFM1044 and lactobacillus casei CCFM711. The effect of relieving metabolic syndrome is significantly better than that of CCFM8630 or CCFM8631 used alone or in combination, especially in the reduction of low-density lipoprotein, total cholesterol and liver triglyceride in serum and glutamic-pyruvic transaminase, glutamic oxalacetic transaminase, alkaline phosphatase, IFN-gamma content in the serum and increase the glutathione and superoxide dismutase content in the liver. The decrease or increase range is improved by 7.91%-837.11% compared with the single or double-bacteria combination formula of the CCFM8630 and the CCFM8631. The four-bacteria combination can achieve the remarkable synergistic interaction.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a compound probiotic and its application, in particular to a compound probiotic for alleviating metabolic syndrome and its application. Background technique [0002] Metabolic syndrome is a group of diseases closely related to obesity, diabetes, hypertension, and cardiovascular disease, and its clinical manifestations are abnormal glucose tolerance, overweight or obesity, dyslipidemia, and hypertension. Numerous studies have shown that metabolic syndrome is a major contributor to cardiovascular disease, and people with metabolic syndrome are also more likely to develop inflammation and are also prone to other diseases, such as polycystic ovary syndrome, fatty liver, cholesterol gallstones, Asthma, sleep disturbances, and certain cancers. In addition, metabolic syndrome is significantly associated with the prevalence and mortality of coronary atherosclerotic heart disease ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23L33/00A61K35/747A61P3/00A61K35/745C12R1/01C12R1/225C12R1/245
CPCA61P3/00A23L33/00A23L33/135A61K35/745A61K35/747C12N1/20A23V2002/00A61K2300/00A23V2200/30A23V2200/3262A23V2400/513A23V2400/173A23V2400/175A23V2400/125A61K9/19A61K9/2004A61K9/4841A61K9/0095A61K9/1605A61K9/10A61K47/26
Inventor 马方励陈卫王刚朱晓风王园园肖俊勇张灏赵建新
Owner INFINITUS (CHINA) CO LTD
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