Preparation method of hybrid liposome containing targeted ester polypeptide

Abstract

The invention relates to a preparation method of a hybrid liposome containing targeted ester polypeptide, and belongs to the field of pharmacy. The preparation method comprises the following steps of:firstly, weighing and placing lecithin, cholesterol and ester polypeptide in a glass container, and adding absolute ethyl alcohol into the glass container for dissolution to obtain a mixed solution;secondly, adding curcumin absolute ethyl alcohol solution into the mixed solution, uniformly mixing, and removing the organic phase by decompression evaporation on a rotary evaporator until a uniformand transparent film is formed on the bottle wall; then adding phosphate buffer salt solution while it is hot to elute and disperse the film, carrying out water bath normal pressure rotary hydration,and carrying out ultrasonic dissolution to obtain a hybrid liposome solution. According to the preparation method, an amphipathic polypeptide containing RGD polypeptide and C12 sequence is used as a targeted material, curcumin is used as a model medicament, and the prepared liposome has the advantages of high encapsulation rate, uniform particle size distribution, good stability, safety and low toxicity.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular relates to a method for preparing a hybrid liposome containing targeting ester polypeptides. Background technique [0002] Liposomes are microvesicles formed by encapsulating drugs in lipid bilayers. Because of its slow release and low toxicity, it plays an important role in the drug delivery system. Liposomes can obtain different targeted drug delivery or sustained and controlled release purposes through their own properties or surface modification. Curcumin (CUR) is a phenolic compound extracted from turmeric, a plant of the ginger family, and is the main active ingredient of the traditional Chinese medicine turmeric. Studies have shown that CUR is a safe and effective drug with multiple functions such as anti-cancer, anti-inflammatory and anti-oxidation. Among them, curcumin is used for the prevention and treatment of tumors, and has the effect on the production, proliferation and m...

AI Technical Summary

Problems solved by technology

[0005] In view of the above situation, the object of the present invention is to provide a method for preparing a hybrid liposome containing a targeting ester polypeptide, which uses an amphiphilic polypeptide containing an RGD polypeptide and a C12 sequence as a targeting material, Curcumin was used as a model drug to prepare liposomes, which solved the problems of difficult administration and low bioavailability of hydrophobic drugs due to low solubility in water. At the same time, the prepared liposomes had high encapsulation efficiency, uniform particle size distribution, and stable Advantages of good sex, safety and low toxicity

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