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Method for producing totally fermented bacterial cellulose membrane containing mushroom fermentation extract

A technology of bacterial cellulose membrane and bacterial cellulose, which is applied in the field of fully fermented bacterial cellulose membrane, can solve the problems of complicated process and long production cycle, and achieve the effects of simplified process flow, high production efficiency and low technical difficulty

Active Publication Date: 2019-04-19
SOUTH CHINA INST OF COLLABORATIVE INNOVATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of this application cosmetic requires different production processes of bacterial cellulose film and mushroom extract, which has the disadvantages of long production cycle and complicated process.

Method used

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  • Method for producing totally fermented bacterial cellulose membrane containing mushroom fermentation extract

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A method for one-step production of a fully fermented bacterial cellulose film containing a mushroom fermentation extract, comprising the following steps:

[0054] Step A: Crush the mushrooms and beat them into a paste, add coconut liquid, mushrooms: coconut liquid = 1:1 (w / w), sterilize at 95°C for 1 minute;

[0055] Step B: Inoculate the lactic acid bacteria and yeast in the sterilized culture obtained in step A with an inoculum volume of 1% (w / v), and culture at 30° C. for 72 hours;

[0056] Step C: Inoculate bacterial cellulose-producing bacteria with an inoculum volume of 10% (w / v) in the culture obtained in step B, and culture it statically in a sterilized shallow dish at 32° C. for 120 hours;

[0057] Step D: cutting and forming the bacterial cellulose film obtained in step C on a forming machine, and collecting the formed residual liquid;

[0058] Step E: Put the molded bacterial cellulose film obtained in step D and the residual liquid into sterilization bags ...

Embodiment 2

[0067] A method for one-step production of a fully fermented bacterial cellulose film containing a mushroom fermentation extract, comprising the following steps:

[0068] Step A: Crush the mushrooms and beat them into a paste, add coconut liquid, mushrooms: coconut liquid = 1:5 (w / w), sterilize at 90°C for 5 minutes;

[0069] Step B: inoculate the lactic acid bacteria and yeast in the sterilized culture obtained in step A with an inoculum volume of 20% (w / v), and culture at 20° C. for 48 hours;

[0070] Step C: Inoculate bacterial cellulose-producing bacteria at an inoculum volume of 5% (w / v) into the culture obtained in step B, and culture it statically in a sterilized shallow dish at 28° C. for 96 hours;

[0071] Step D: cutting and forming the bacterial cellulose film obtained in step C on a forming machine, and collecting the formed residual liquid;

[0072] Step E: Put the molded bacterial cellulose film obtained in step D and the residual liquid into sterilization bags ...

Embodiment 3

[0081] A method for one-step production of a fully fermented bacterial cellulose film containing a mushroom fermentation extract, comprising the following steps:

[0082] Step A: Crush the mushrooms and beat them into a paste, add coconut liquid, mushrooms: coconut liquid = 1:9 (w / w), sterilize at 92°C for 3 minutes;

[0083] Step B: Inoculate the lactic acid bacteria and yeast in the sterilized culture obtained in step A with an inoculum of 8% (w / v) by volume, and culture at 37° C. for 10 hours;

[0084] Step C: Inoculate bacterial cellulose-producing bacteria at an inoculum volume of 20% (w / v) into the culture obtained in step B, and culture it statically in a sterilized shallow dish at 25° C. for 24 hours;

[0085] Step D: cutting and forming the bacterial cellulose film obtained in step C on a forming machine, and collecting the formed residual liquid;

[0086] Step E: Put the molded bacterial cellulose film obtained in step D and the residual liquid into sterilization ba...

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Abstract

The invention discloses a method for producing a totally fermented bacterial cellulose membrane containing a mushroom fermentation extract, and relates to the technical field of biological fermentation. The method has the advantages that active ingredients of mushrooms are extracted using a microbial synergistic fermentation method, the reaction conditions are mild, no chemical reagent is needed,the effective ingredients of the mushroom raw materials can be fully extracted and separated, a large number of active enzymes are contained in fermentation broth, the functionality of natural ingredients of the mushrooms can be maintained to the maximum extent, new effective ingredients might be generated in the biological conversion process to further improve the functionality of the mushroom raw materials, the natural, safe and totally fermented bacterial cellulose membrane which can be directly used, contains the mushroom fermentation multifunctional extract, has no additive and irritationis obtained, the production process is simplified, the production cycle is shortened, the production cost is reduced, and the production efficiency is improved; wasted coconut water and wasted coconut pulp liquid are taken as fermentation additives, wastes are turned into treasures, and the waste of resources is reduced.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a method for producing a fully fermented bacterial cellulose film containing mushroom fermentation extracts in one step. Background technique [0002] Bacterial Cellulose (BC) is a three-dimensional network microfiber with a fiber diameter of about 20-100 nm and a pore structure size of about 0.5-1.0 μm synthesized by microorganisms such as Acetobacter xylinus. BC has a similar chemical structure to natural fibers, but compared with natural fibers, BC has no complex components such as hemicellulose and lignin, so BC has the characteristics of high crystallinity, high mechanical strength, high purity and high degree of polymerization. . Due to its special structure and source, BC also has unique properties such as hydrophilicity, strong absorption capacity, non-allergic, easy biodegradation, and good biocompatibility. Based on its unique nano-characteristics, BC ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P19/04C08J5/18C08L1/02C12R1/02C12R1/24C12R1/25C12R1/245C12R1/225C12R1/23C12R1/46C12R1/01C12R1/72C12R1/865C12R1/645
CPCC08J5/18C08J2301/02C12P19/04C12P39/00
Inventor 王云杨继国刘玮任杰刘丹夏晚霞
Owner SOUTH CHINA INST OF COLLABORATIVE INNOVATION