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Composition for alleviating skin irritation and skin inflammation caused by fine dust, containing, as active ingredient, mixture of holy basil, mentha canadensis, and azadirachta indica extracts

A technology for skin inflammation and skin irritation, which is applied in the field of skin external preparation compositions for skin irritation relief and skin inflammation relief, can solve the problems of damaged epidermal barrier function, exacerbation of allergic dermatitis, etc., and achieve the goal of alleviating skin irritation and skin inflammation Effect

Inactive Publication Date: 2019-04-19
COSMAX INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, fine dust in air pollutants has been reported to impair the epidermal barrier function and aggravate atopic dermatitis

Method used

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  • Composition for alleviating skin irritation and skin inflammation caused by fine dust, containing, as active ingredient, mixture of holy basil, mentha canadensis, and azadirachta indica extracts
  • Composition for alleviating skin irritation and skin inflammation caused by fine dust, containing, as active ingredient, mixture of holy basil, mentha canadensis, and azadirachta indica extracts
  • Composition for alleviating skin irritation and skin inflammation caused by fine dust, containing, as active ingredient, mixture of holy basil, mentha canadensis, and azadirachta indica extracts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Preparation of an extraction mixture of holy basil, mint and neem

[0039] After air-dried holy basil, mint, and neem leaves were powdered, 33.3g were taken, mixed with a total of 99.9g, as the extraction solvent, 1L was immersed in 70 wt% ethanol aqueous solution, and then Leave it at room temperature for 24 hours to obtain an extract. After filtering the obtained extract with filter paper, the filtrate is concentrated under reduced pressure using a rotary evaporator to obtain a concentrated extract mixture.

experiment example 1

[0043] Experimental example 1: Evaluation of anti-inflammatory effect

[0044] In order to evaluate the anti-inflammatory effect of the mixed extract, IL-6 and IL-8, which are inflammation-inducing cytokines, were treated with fine dust (PM10), and their expression levels were measured. In more detail, the HaCaT cells, which are keratinocytes, were treated with fine dust, and the individual extracts of holy basil, peppermint, and neem prepared in Comparative Example 1 and the extract obtained in Example 1 were used in equal proportions. The final concentration of the mixture reached 10 μg / ml, and was diluted in the medium, thereby culturing for 4 hours. Next, in the cell, mRNA is extracted, synthesized with cDNA, and real-time polymerase chain reaction (PCR) is performed using the target template (primer) to finally evaluate the gene expression level of inflammatory cytokines. Show the result in figure 1 with figure 2 in.

[0045] The result is like figure 1 with figure 2 As s...

experiment example 2

[0046] Experimental Example 2: Evaluation of the Relief Effect of Epidermal Stimulation

[0047] In order to evaluate the effect of relieving skin epidermal irritation, three-dimensional cultured artificial skin tissue was used to evaluate the effect of relieving the skin layer that was stimulated and damaged by fine dust. First, 50 μg / ml fine dust (PM10) was applied to the surface of the artificial skin, and the mixed extract obtained in Example 1 was treated on the culture medium at a concentration of 10 μg / ml. After 24 hours of additional culture, after fixation in formalin, a paraffin block was prepared for H&E staining. Thus, the morphology of the stratum corneum and keratinocytes of the epidermal layer was measured. And, the measurement results are shown in image 3 in.

[0048] The result is like image 3 As shown, it was confirmed that the stratum corneum of the artificial skin tissue damaged by the fine dust treatment was relieved. In addition, it was confirmed that th...

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PUM

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Abstract

The present invention relates to an external use skin preparation composition for alleviating skin inflammation, containing a natural extract mixture as an active ingredient. According to the presentinvention, an external use skin preparation composition, containing, as active ingredients, a mixture of holy basil, Mentha canadensis and Azadirachta indica extracts, inhibits the expression of inflammatory cytokines (IL-6, IL-8), which is caused by fine dust, thereby alleviating skin irritation and skin inflammation caused by fine dust, and thus the composition can be ultimately and effectivelyapplied to a use such as acne prevention and alleviation, flushing alleviation, and dermatitis prevention and alleviation.

Description

Technical field [0001] The present invention relates to a skin external preparation composition containing a natural mixture having skin irritation relief and skin inflammation relief activities as an active ingredient, and more specifically, to a skin irritation relief and skin inflammation that includes an extract mixture of holy basil, peppermint, and neem An external preparation composition for skin relief. Background technique [0002] One of the most important functions of the skin is its protective function as a barrier. The skin directly exposed to the external environment is the most important first line of defense to prevent the loss of body fluids and protect the body from harmful environments. In addition, it performs a barrier function against toxic substances or microorganisms, physical stimuli, or ultraviolet rays. However, if this skin barrier function is stimulated by a variety of external and internal skin, the skin barrier is damaged, causing the skin to beco...

Claims

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Application Information

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IPC IPC(8): A61K36/53A61K36/534A61K36/58A61K8/9789A61K8/02A61Q19/00
CPCA61K8/02A61K8/97A61K36/53A61K36/534A61K36/58A61Q19/00A61K2300/00A61K8/9789
Inventor 李敬银姜承贤金延俊卢旻秀李相国
Owner COSMAX INC
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