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Method for preparing harmful heavy metal adsorbent by utilizing active polysaccharide of Kocuria

A technology of Corkella and active polysaccharide, applied in the field of water pollution treatment, can solve problems such as unsatisfactory effect of heavy metal adsorption and removal, and achieve the effects of high preparation efficiency and yield, stable performance, and simple overall steps

Active Publication Date: 2019-04-23
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to solve the problem of unsatisfactory adsorption and removal of harmful heavy metals in existing tap water, the present invention provides a method for preparing harmful heavy metal adsorbents by using active polysaccharides from Cochlear bacteria

Method used

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  • Method for preparing harmful heavy metal adsorbent by utilizing active polysaccharide of Kocuria

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Embodiment 1

[0065] A method for preparing a harmful heavy metal adsorbent by using the active polysaccharide of Corkia bacterium, said method comprising the following steps:

[0066] 1) Cultivate strains: Prepare 2.7L of microbial culture fluid, which contains the following substances in weight percentage: 5% potato juice, 1% clam broth, 0.06% dipotassium hydrogen phosphate and 2% glucose, and the balance is aged Seawater: Inoculate the selected Cochlear bacteria into the microbial culture solution to form a bacterial solution, and cultivate the selected strains of the bacterial solution. The cultivation conditions are: in an environment of 30°C, cultivated on a shaker at 150r / min for 3d ;

[0067] 2) Extraction of flocculant: Concentrate the cultivated bacterial liquid, concentrate the bacterial liquid to 12% of the original volume during the concentration process, put the concentrated bacterial liquid into a 2000 Dalton dialysis bag, dialyze for 60 hours, and then dialyze The final ret...

Embodiment 2

[0070] A method for preparing a harmful heavy metal adsorbent by using the active polysaccharide of Corkia bacterium, said method comprising the following steps:

[0071] 1) Cultivate strains: prepare 3.0L of microbial culture fluid, which contains the following substances in weight percent: 8% potato juice, 3% clam broth, 0.18% dipotassium hydrogen phosphate and 3% glucose, and the balance is aged Inoculate the seawater; inoculate the selected Cochlear bacteria into the microbial culture solution to form a bacterial solution, and cultivate the selected strains of the bacterial solution. The cultivation conditions are: in an environment of 25°C, cultivate on a shaking table at 200r / min 5d;

[0072] 2) Extraction of flocculant: Concentrate the cultivated bacterial liquid, concentrate the bacterial liquid to 9% of the original volume during the concentration process, put the concentrated bacterial liquid into a 5000 Dalton dialysis bag, dialyze for 36 hours, and then dialyze Th...

Embodiment 3

[0075] A method for preparing a harmful heavy metal adsorbent by using the active polysaccharide of Corkia bacterium, said method comprising the following steps:

[0076] 1) Cultivate bacterial strains: prepare 2.7L of microbial culture solution, which contains the following substances in weight percentage: 6.5% of potato juice, 1.5% of clam broth, 0.12% of dipotassium hydrogen phosphate and 2.25% of glucose, and the balance is old Thin seawater; Inoculate the selected Cochlear bacteria into the microbial culture solution to form a bacterial solution, and cultivate the selected strains of the bacterial solution. The cultivation conditions are: in an environment of 27°C, cultivate on a shaking table at 180r / min 4d;

[0077] 2) Extraction of flocculant: Concentrate the cultivated bacterial liquid, concentrate the bacterial liquid to 300mL during the concentration process, put the concentrated bacterial liquid into a 2000 Dalton dialysis bag, dialyze for 48 hours, and then remove...

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Abstract

The invention relates to the technical field of water pollution treatment. In order to solve the problem that the effect of adsorbing and removing heavy metals from tap water is non-ideal, the invention provides a method for preparing a harmful heavy metal adsorbent by utilizing an active polysaccharide of Kocuria. The method comprises the following steps: (1) culturing strains, namely, inoculating the selected Kocuria strains into a microbial medium to form a bacteria solution, and culturing the selected strains in the bacteria solution; (2) extracting a flocculant, namely, concentrating thecultured bacteria solution, performing dialysis and centrifugal separation, and extracting to obtain the microbial flocculant; and (3) preparing an adsorbent, namely, dissolving the microbial flocculant in a mixed aqueous solution of agar and starch, and preparing to obtain the harmful heavy metal adsorbent after the solution is solidified. The method disclosed by the invention is simple in overall steps; both the preparation efficiency and the yield are high; and the prepared adsorbent has the advantages of safety and harmlessness, can be directly added into drinking water to adsorb and remove harmful heavy metal components, and has high adsorption efficiency and good adsorption effect on the harmful heavy metal components.

Description

technical field [0001] The invention relates to the technical field of water pollution treatment, in particular to a method for preparing a harmful heavy metal adsorbent by utilizing the active polysaccharide of Cochlear bacteria. Background technique [0002] Most of the water used by people in production and life is tap water, which is mainly obtained by treating water drawn from groundwater or river water by water plants. Because some water sources are affected by industrial or domestic sewage discharges, the heavy metal content exceeds the standard. Although it is treated by a water plant before being used for human and livestock consumption, the content of some heavy metals is still at a high level, and some even exceed the standard; in addition, urban tap water pipelines Old age will also lead to the infiltration of heavy metals such as lead into the water body, causing the heavy metal content to exceed the standard. [0003] As Singapore Lianhe Zaobao.com reported on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/30B01J20/24C02F1/28C02F101/20
CPCB01J20/24B01J2220/4825C02F1/286C02F2101/20C02F2101/206
Inventor 穆军
Owner ZHEJIANG OCEAN UNIV
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