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Plant tissue silicon distribution solid phase fluorescence microscopic detection method

A solid-phase fluorescence and microscopic detection technology, used in fluorescence/phosphorescence, measuring devices, analytical materials, etc., can solve problems such as poor specificity, unstable supply, and difficulty in observing silicon distribution characteristics, and achieve the protection of ashing phase. , the effect of high specific gravity

Active Publication Date: 2019-04-23
DRY LAND FARMING INST OF HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. The integrity is not good
At present, dry ashing method is often used to observe the microstructure of silicon. The important components of ash are silicon (silicon dioxide) and some metal oxides. By burning out organic matter, gray-white silicon plants of different sizes and shapes can be observed under a microscope. However, during the combustion process, it is difficult to maintain the arrangement of plant tissues. This method can only observe the crystallization of silicon cells, and it is not easy to observe the distribution characteristics of silicon. It can only be used to observe the morphology of stone cells. Unable to perform silicon positioning
[0007] 2. Poor specificity
The disadvantage is that the reagents are expensive and the supply is not stable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A solid-phase fluorescence microscopy detection method for silicon distribution in plant tissue provided by the invention comprises the following steps:

[0036] The first step is to prepare the sheet test material

[0037] Collect the leaves or stalks, wipe or wash the surface of the leaves or stalks with water, remove the dust on the surface of the leaves or stalks that may interfere with the experiment, slice the leaves or stalks horizontally or longitudinally by hand, and make them into pieces with a thickness of less than 1 mm and a length of 1 mm. Sheet specimens whose width is smaller than the length and width of the quartz glass slide;

[0038] The second step, the concentrated sulfuric acid softens the flake test material

[0039] Set the flake test material on a quartz glass slide, add concentrated sulfuric acid dropwise to the flake test material, after the concentrated sulfuric acid is evenly distributed on the flake test material, place the quartz glass slid...

Embodiment 2

[0051] A solid-phase fluorescence microscopy detection method for silicon distribution in plant tissue provided by the invention comprises the following steps:

[0052] The first step is to prepare the sheet test material

[0053] Collect the leaves or stalks, wipe or wash the surface of the leaves or stalks with water, remove the dust on the surface of the leaves or stalks that may interfere with the experiment, slice the leaves or stalks horizontally or longitudinally by hand, and make them into pieces with a thickness of less than 1 mm and a length of 1 mm. Sheet specimens whose width is smaller than the length and width of the quartz glass slide;

[0054] The second step, the concentrated sulfuric acid softens the flake test material

[0055] Set the flake test material on a quartz glass slide, add concentrated sulfuric acid dropwise to the flake test material, after the concentrated sulfuric acid is evenly distributed on the flake test material, place the quartz glass sl...

Embodiment 3

[0067] A solid-phase fluorescence microscopy detection method for silicon distribution in plant tissue provided by the invention comprises the following steps:

[0068] The first step is to prepare the sheet test material

[0069] Collect the leaves or stalks, wipe or wash the surface of the leaves or stalks with water, remove the dust on the surface of the leaves or stalks that may interfere with the experiment, slice the leaves or stalks horizontally or longitudinally by hand, and make them into pieces with a thickness of less than 1 mm and a length of 1 mm. Sheet specimens whose width is smaller than the length and width of the quartz glass slide;

[0070] The second step, the concentrated sulfuric acid softens the flake test material

[0071] Set the flake test material on the quartz glass slide, add concentrated sulfuric acid dropwise to the flake test material. Transparent to digest organic matter and reduce gas disturbance during ashing;

[0072] The third step is to...

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Abstract

The invention belongs to the technical field of plant tissue detection, and discloses a plant tissue silicon distribution solid phase fluorescence microscopic detection method. The method has the maintechnical scheme that the method comprises the following steps of making a flaky test material; performing softening treatment on the flaky test materials by concentrated sulfuric acid; cleaning theconcentrated sulfuric acid by pure water; preparing zinc chloride solution; performing soaking treatment on the flaky test materials through zinc chloride solution; performing covering treatment on the flaky test materials by barium chloride; observing the flaky test materials by a fluorescence microscope. The plant tissue silicon distribution solid phase fluorescence microscopic detection methodprovided by the invention has the advantages that the fluorescence characteristics of the zinc silicate are applied to the silicon tissue chemical positioning; the physical and chemical characteristics of the barium chloride are applied for the ash phase fixation in the ashing process. The method does not have the strict requirements on the experiment conditions, medicine concentration and treatment time, and is applicable to the work development in laboratories under the basic conditions of having a reverse fluorescence microscope and a muffle furnace.

Description

technical field [0001] The invention belongs to the technical field of plant tissue detection, and in particular relates to a solid-phase fluorescence microscope detection method for silicon distribution in plant tissue. Background technique [0002] Among the 16 essential elements needed for plant growth, there is no silicon element. However, in the Earth's crust, silicon is the second most abundant element after oxygen, making up 26.4% of the total mass of the Earth's crust. [0003] Silicon does not participate in the metabolism of life. But as a high-abundance element, plants generally contain silicon. Under the action of transpiration, plants absorb the silicon dissolved in the soil in the form of monosilicic acid, and transpiration with water, leaving silicon in the body to deposit in the form of silicon dioxide in the cell and on the cell wall. According to the silicon content of plants, plants can be divided into silicon-loving, medium-silicon and low-silicon plan...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/28
CPCG01N1/28G01N21/6402
Inventor 王雪征茜晓哲王晨阳陈洁庞建周
Owner DRY LAND FARMING INST OF HEBEI ACAD OF AGRI & FORESTRY SCI