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A kind of method that utilizes liquid culture medium to produce the fruiting bodies of Xanthomonas vertical stripes

A technology for the fruiting body and liquid culture medium of Xanthomonas, which is applied in the field of biology, and can solve the problems that it is difficult to obtain mature fruiting bodies of Xanthomonas vertical stripes, so as to achieve the effects of improving bacteria production efficiency, increasing yield, and good quality

Active Publication Date: 2021-06-22
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that most of the laboratory liquid cultures of C. vertical stripes are mycelia, while wild fruiting bodies are mostly born in the wild. Due to the influence of seasons and climates, it is difficult to obtain a large number of mature fruiting bodies of C. vertical stripes. This paper The invention provides a method for cultivating the fruiting body of Xanthomonas vertical stripes in liquid

Method used

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  • A kind of method that utilizes liquid culture medium to produce the fruiting bodies of Xanthomonas vertical stripes
  • A kind of method that utilizes liquid culture medium to produce the fruiting bodies of Xanthomonas vertical stripes
  • A kind of method that utilizes liquid culture medium to produce the fruiting bodies of Xanthomonas vertical stripes

Examples

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Effect test

Embodiment 1

[0044] A method for cultivating the fruiting body of Xanthomonas longitudinal stripes in liquid, comprising the following steps:

[0045] Step 1, prepare liquid culture medium

[0046] Slice 200g of peeled potatoes, then add water until the potatoes are submerged, heat and boil until the potatoes are soft and rotten, filter through three layers of gauze, take the filtrate, and cool slightly to obtain potato juice;

[0047] Weigh 2g of yeast powder, 15g of glucose, 2g of peptone, 2.5g of potassium dihydrogen phosphate, 1.5g of magnesium sulfate, and 10.1g of vitamin B in a beaker, add potato juice that has been cooled to 70-80°C, and stir until all solids Dissolve and dilute to 1L to obtain a liquid medium;

[0048] Step 2, bottling and sterilization

[0049] Divide the liquid medium into each culture container, and keep the filling volume at a medium thickness of 2cm;

[0050] Place the culture container containing the liquid culture medium in a high-pressure container, and...

Embodiment 2

[0056] A method for cultivating the fruiting body of Xanthomonas longitudinal stripes in liquid, comprising the following steps:

[0057] Step 1, prepare liquid culture medium

[0058] Slice 200g of peeled potatoes, then add water until the potatoes are submerged, heat and boil until the potatoes are soft and rotten, filter through three layers of gauze, take the filtrate, and cool slightly to obtain potato juice;

[0059] Weigh 3g of yeast powder, 25g of glucose, 3g of peptone, 3.5g of potassium dihydrogen phosphate, 2g of magnesium sulfate, and 10.5g of vitamin B into a beaker, add potato juice that has been cooled to 70-80°C, and stir until all solids are dissolved , and set the volume to 1L to obtain a liquid medium;

[0060] Step 2, bottling and sterilization

[0061] Divide the liquid medium into each culture container, and keep the filling volume at a medium thickness of 3cm;

[0062] Place the culture container containing the liquid culture medium in a high-pressure...

Embodiment 3

[0068] A method for cultivating the fruiting body of Xanthomonas longitudinal stripes in liquid, comprising the following steps:

[0069] Step 1, prepare liquid culture medium

[0070] Slice 200g of peeled potatoes, then add water until the potatoes are submerged, heat and boil until the potatoes are soft and rotten, filter through three layers of gauze, take the filtrate, and cool slightly to obtain potato juice;

[0071] Weigh 2.5g of yeast powder, 20g of glucose, 2.5g of peptone, 3g of potassium dihydrogen phosphate, 1.8g of magnesium sulfate, and 10.3g of vitamin B in a beaker, add potato juice that has been cooled to 70-80°C, and stir until solid Dissolve it completely, and dilute to 1L to obtain a liquid medium;

[0072] Step 2, bottling and sterilization

[0073] Divide the liquid culture medium into each culture container, and keep the filling volume at a medium thickness of 4cm;

[0074] Place the culture container containing the liquid culture medium in a high-pre...

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Abstract

The invention provides a method for cultivating the fruiting body of Xanthomonas vertical stripes in a liquid. The steps are as follows: preparing a liquid medium: peeling potato slices, adding water and boiling them, filtering and taking the filtrate to cool slightly; weighing yeast powder, glucose, peptone, diphosphate Potassium hydrogen, magnesium sulfate, vitamin B1, add potato juice, stir until dissolved to obtain a liquid culture medium; bottling and sterilization; inoculation: in a sterile environment, the liquid culture medium is inserted into the liquid culture medium with the vertical stripes of Xanthomonas The solid medium of mycelium or one or two fruiting bodies; static culture in a cool place at 25-30°C, the lawn will start to grow in about ten days, and the fruiting bodies will start to grow after another week. It may also include the step of inoculating and culturing the fruiting body again with its liquid culture medium after picking the fruiting body of C. vertical stripes. The method of the invention has simple process and is easy to implement, and the hyphae can directly grow into fruiting bodies in liquid culture medium, which greatly improves the bacteria production efficiency, and is denser than the fruiting bodies cultured in solid, thus providing a guarantee for increasing yield.

Description

technical field [0001] The invention belongs to the technical field of biology, and in particular relates to a method for directly growing the fruiting body of C. striata in liquid culture medium. Background technique [0002] Xylaria striata (Xylaria striata Pat.1887), belongs to Ascomycota, Panmycotina, Faecium, Xylaria, Xylariales, Xylariaceae, Xylaria Hill ex Schrank). The fungus is an endophytic fungus that grows buried or superficially on the decaying bark and living roots of broad-leaved trees in summer. Various studies have shown that the fungus of the genus Anthracera has great development value. According to literature research reports, in addition to antibacterial, anti-oxidant, anti-tumor and enzyme inhibitory activities, Xylella has many activities, and Xylella is also used in mental and psychological diseases, nervous system diseases and digestive system diseases. [0003] After the inventor obtained the wild strain of C. vertical stripes, he conducted a sys...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00A01G18/20
Inventor 黄毅袁诗俊贺新生袁小红简甜甜李唯一
Owner SOUTHWEAT UNIV OF SCI & TECH
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