Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting florfenicol and metabolite florfenicol amine in pig urine

A technology of florfenicol and florfenicol, which is applied in the field of veterinary drug residue detection, can solve problems such as increasing the difficulty of processing procedures, and achieve the effects of reliable quantitative analysis results, good specificity, and reduced detection costs.

Inactive Publication Date: 2019-05-03
WUHAN INST OF BIOENG
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existence of these differences significantly increases the difficulty of selecting the best sample preparation procedure

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting florfenicol and metabolite florfenicol amine in pig urine
  • Method for detecting florfenicol and metabolite florfenicol amine in pig urine
  • Method for detecting florfenicol and metabolite florfenicol amine in pig urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The comparison of various sample purification techniques purification effect of embodiment 1

[0043] 1. Method

[0044] (1) Accurately measure 2 mL of fresh blank pig urine, add 0.06 mL of 2.5N sodium hydroxide solution, and vortex for 1 min.

[0045] (2) Add 8 mL of ethyl acetate to the alkalized urine, vortex for 2 min, centrifuge at 5000 g for 5 min, and transfer the ethyl acetate to another clean centrifuge tube.

[0046] (3) Repeat the above operation once with an equal amount of ethyl acetate, and combine the ethyl acetate.

[0047] (4) Transfer ethyl acetate in batches to centrifuge tubes containing 0.6 mL of 5% glacial acetic acid solution, and evaporate the ethyl acetate to dryness with air flow at 50°C.

[0048] (5) The remaining glacial acetic acid solution was treated in the following three ways (repeat 5 samples for each method): ① without purification, dry the glacial acetic acid solution in air flow at 45°C, add 1 mL of mobile phase to redissolve the r...

Embodiment 2

[0052] The accuracy and precision test of florfenicol and florfenicol amine in the pig urine of embodiment 2

[0053] 1. Method

[0054] (1) Accurately measure 2 mL of pig urine, add 0.25, 0.5 and 1 μg / mL florfenicol and florfenicol amine to it, repeat 5 samples for each concentration, and repeat three batches continuously.

[0055] (3) Process the above sample according to the extraction, concentration and thin-layer chromatography purification procedures shown in Example 1, and analyze according to the chromatographic conditions shown in Example 1. Florfenicol and florfenicol amine are quantified using a standard curve, and a series of concentration florfenicol amine standard products are detected by high performance liquid chromatography, with the concentration of florfenicol amine as the abscissa and the peak area as the ordinate, Obtain the regression equation y=143064x-1045.3 of Florfenicol and the regression equation y=275826x+1888.8 of Florfenicol amine, wherein x is ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting florfenicol and metabolite florfenicol amine in pig urine, and belongs to the field of clinical pharmacokinetics of veterinary antibacterial drugs. The method includes the following steps: adding proper amount of a sodium hydroxide solution in pig urine to adjust pH to alkalinity; performing extraction by ethyl acetate; performing condensation on an extract product, and taking a mixed liquid with a ratio of dichloromethane : acetone : ammonia water being (1-6) : (4-9) : (0.05-0.5) as a developing solution to perform thin layer chromatography purifying; and performing detection by adopting high performance liquid chromatography. The method effectively removes endogenous substances contained in the urine extract product by using thin layer chromatography, so that the interference on chromatographic analysis can be avoided, detection costs can be reduced, and the using amount of an organic solvent can be decreased; and the method can combinethe thin layer chromatography and high-performance liquid chromatography, so that deficiency of a detector matching a high performance liquid chromatograph on specificity can be made up, and applications of the high-performance liquid chromatography in clinical pharmacokinetics can be widened.

Description

technical field [0001] The invention belongs to the field of veterinary drug residue detection, in particular to a method for detecting florfenicol and its metabolite florfenicol amine in pig urine. Background technique [0002] Florfenicol has the advantages of broad spectrum, high efficiency and low toxicity, and is widely used in my country to prevent and treat respiratory tract infections in pigs. However, unreasonable use may lead to treatment failure, induce the generation of drug resistance, and shorten the service life of florfenicol; it may also cause the residues of florfenicol and its metabolites in the edible tissue of pigs to exceed the maximum residue limit, which is harmful Consumer health and sustainable farming. In order to ensure the control effect and avoid the food safety problems caused by the abuse of florfenicol, it is very important to formulate a reasonable dosing regimen. [0003] The clearance of florfenicol in pigs is closely related to its anti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/90G01N30/94G01N30/95G01N30/02G01N30/06G01N30/14G01N30/34G01N30/74G01N30/86G01N30/88
Inventor 杨波王前勇高进东周丹娜李亚超
Owner WUHAN INST OF BIOENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com