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Manufacturing method of hangover-eliminating enzyme powder and composition for relieving hangover comprising thereof

The technology of a hangover enzyme and composition is applied in the field of the preparation of a hangover-eliminating enzyme powder and a composition for eliminating hangover containing the component, which can solve the problems of insufficient effect, inability to eliminate hangover and detoxification, and little effect.

Active Publication Date: 2019-05-07
艾美科健株式会社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]However, traditional hangover drinks focus on the bioactive ingredients that inhibit or promote the activity of enzymes in the liver, namely ADH, and mainly extract Chinese medicinal herbs It is prepared as a beverage, which has little effect or temporarily relieves symptoms, cannot fundamentally eliminate hangover or detoxify alcohol, and has no effect or insufficient effect on head and gastrointestinal system problems such as abdominal pain, headache, and nausea after drinking alcohol

Method used

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  • Manufacturing method of hangover-eliminating enzyme powder and composition for relieving hangover comprising thereof
  • Manufacturing method of hangover-eliminating enzyme powder and composition for relieving hangover comprising thereof
  • Manufacturing method of hangover-eliminating enzyme powder and composition for relieving hangover comprising thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0083] Hangover Elimination Enzyme Powder

[0084] An acetic acid strain (Acetobacter pasteurianus VA2, Acetobacter pasteurianus VA2) was subjected to acetic acid fermentation to express ADH and ALDH, and to prepare an enzyme complex comprising the ADH and ALDH. The specific method is as follows.

[0085] The acetic acid bacteria were cultured for 16 to 24 hours in a pre-medium of yeast extract powder 0.5%, D-mannitol 2.5% and peptone 0.3%. Mix yeast extract powder 0.5% to 1%, soytone 0.5% to 1.5%, hydrous crystalline glucose 2.5% to 7.5%, manganese sulfate 0.01% to 0.05%, and mix fermentation alcohol 1% to 5% in this medium , inoculate the pre-cultured strain, and cultivate it at 25 to 30° C. at 200 to 300 rpm for 24 to 36 hours. After cultivation, the pH is preferably 3.5 to 4.0, and the acetic acid concentration is preferably 2.0 to 4.0% by weight. Then the supernatant was removed by centrifugation (10,000×g, 20 minutes), and the precipitate was washed 2 to 3 times with...

preparation example 2

[0087] Hangover Elimination Enzyme Complex

[0088] A hangover-eliminating enzyme complex was prepared by adding grain fermented enzyme powder (Application No. 10-2017-0080405) to the hangover-eliminating enzyme powder obtained in . Then, 103 mg / kg of hangover-eliminating enzyme powder and 103 mg / kg of grain fermentation enzyme powder (1:1 weight ratio), hereinafter expressed as CA+FG, were administered to the animal model.

Embodiment 1

[0092] Determination of Enzyme Activity of Hangover Enzyme Powder

[0093] Activity Analysis Method of Alcohol Dehydrogenase

[0094] First, 200 μl of the above-prepared sample was placed in an E-tube, and the enzyme activity was removed by heating at 100° C. for 30 minutes or more. 1.3 mL of 50 mM sodium phosphate buffer (pH 8.8, sodium phosphate buffer) and 1.5 mL of 15 mM β-NAD solution were mixed with 0.1 mL of 95% (v / v) ethanol to prepare a substrate reaction solution so that the total solution was 2.9mL.

[0095] 6.67 μl of the sample and 193.3 μl of the prepared reaction solution were mixed and dispensed into a 96-well microplate (blank = enzyme solution heated at 100° C. for 30 minutes). After that, after measuring the absorbance at 340 nm and 25° C. for 0-10 minutes using a multiple reader (Multiple reader), the activity of alcohol dehydrogenase was analyzed using the following .

[0096] Activity analysis method of acetaldehyde dehydrogenase

[0097] First, 2...

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Abstract

The present invention relates to a manufacturing method of a hangover relief enzyme powder and a composition for relieving hangover containing the same, and more specifically, to a manufacturing method of a hangover relief enzyme powder, and a pharmaceutical composition and a food composition containing the hangover relief enzyme powder as an active ingredient for preventing and relieving hangover, wherein the manufacturing method of a hangover relief enzyme powder comprises the following steps of: a) culturing an Acetobacter Pasteurinus VA2 strain in a solid medium containing an enzyme extract powder, and then inoculating the strain into a liquid medium, in which an enzyme extract powder and fermented alcohol are mixed, to ferment the same; b) obtaining acetobacter by centrifuging a culture medium fermented in the step a) and by removing supernatant therefrom; c) pulverizing the acetobacter obtained in the step b) and mixing excipient thereto; and d) drying and pulverizing the mixtureof the step c). The manufacturing method of the present invention can be used to relieve hangover and prevent damage to liver due to drinking by manufacturing a composition exhibiting enzyme activities of alcohol dehydrogenase enzyme (ADH) and acetaldehyde dehydrogenase enzyme (ALDH) from acetobacter having excellent acetic acid production ability.

Description

technical field [0001] The present invention relates to a preparation method of hangover-eliminating enzyme powder and a hangover-eliminating composition containing the composition {A manufacturing method of hangover-eliminating enzyme powder and a composition for relieving hangover comprising thereof}, more specifically, to a A method for preparing hangover enzyme powder, the method comprising the following steps: a) after cultivating Acetobacter pasteurianus VA2 (Acetobacter pasteurianus VA2) bacterial strain in a solid medium containing yeast extract powder, mixing the yeast extract powder and fermenting The step of inoculating the strain in alcoholic liquid culture medium and carrying out fermentation; b) centrifuging the culture liquid fermented in the step a) and removing the supernatant to obtain the acetic acid bacterium; c) crushing the step obtained in the step b). the step of mixing excipients; and d) the step of drying and powdering the mixture of the step c), and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61K9/14A61P39/02A61P1/16A23L33/135
CPCA61K38/43A23L33/135A23L33/14A23V2200/334A23V2002/00
Inventor 慎镛喆朴哲金利洙金爱香沈在民
Owner 艾美科健株式会社
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