Method for stirring assisted ultrasonic extraction of Bletilla striata polysaccharides
A polysaccharide and ultrasonic technology, applied in the field of biopharmaceuticals, can solve the problems of high energy consumption and low efficiency, and achieve the effect of low energy consumption and easy extraction
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[0011] A method for extracting polysaccharides from Baiji with stirring-assisted ultrasound, comprising the following steps.
[0012] Step 1: After washing, slicing and drying fresh bletilla tubers, they are pulverized by a pulverizer to obtain bletilla powder with a particle size of less than 80 meshes, which are sealed and stored for later use.
[0013] Step 2. By weight, take 1 part of white powder prepared in step 1 and put it into a blender, add 150 parts of drinking water into the blender, start ultrasonication and stir for 40 min at the same time to obtain a mixed material liquid. Ultrasonic frequency is 53kHz, ultrasonic power is 2800W / m 2 , The speed of the mixer is 300 r / min.
[0014] Step 3 Centrifuge the mixed material liquid in step 2 to obtain an extract, and the centrifugation speed is 4000r / min. Gradually add ethanol to the extract, so that the concentration of ethanol in the extract reaches 80%, and the polysaccharide is precipitated.
[0015] The above met...
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